"Mind the gap" in immunohistochemical techniques used in pathology of pituitary adenomas

Dumbravă, Mona

doi:10.4183/aeb.2005.411

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…It has been suggested that these tumors do not cause any hormone-associated clinical disorder either due to inactive hormone expression or due to defect in secretion despite normal hormone expression. It has also been hypothesized that NFAs catabolize the hormones in lysosomes (5). In our study, there were no clinical symptoms of Cushing's syndrome or elevated PRL in patients with pituitary adenomas that stained positive for ACTH and PRL, respectively.…”

Section: Discussioncontrasting

confidence: 40%

“…Utilizing more sensitive IHC techniques decreased the incidence of non-immunoreactive pituitary adenomas (3,4). Even though the hormone immunoreactivity is commonly observed in pituitary Büyüme Hormonu Salgılayan Adenomlarda İmmünohistokimyasal İnceleme Sonuçları ile Laboratuvar Bulguları Arasındaki Korelasyon Immunostaining Results of Growth Hormone Secreting Adenomas and Their Correlation with Laboratory Findings adenomas, this finding does not always correlate with the laboratory and clinical findings (5). In this study, we investigated the correlation between IHC results and clinical and laboratory findings in 67 patients who were operated due to GH-secreting adenoma.…”

Section: Introductionmentioning

confidence: 99%

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Immunostaining Results of Growth Hormone Secreting Adenomas and Their Correlation with Laboratory Findings

Tuna¹,

Karakılıç²,

Basaran³

et al. 2016

tjem

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Hipofiz adenomlarının sınıflamasında daha sensitif immünohistokimyasal ve ultrastrüktürel tekniklerin kullanılması ile hormon immünreaktivite gösteren adenomların insidansında artış olduğu belirtilmektedir. Bu çalışmada akromegali nedeniyle opere edilen hastaların immünohistokimyasal inceleme sonuçları ile klinik ve laboratuvar bulguları arasındaki ilişkiyi araştırdık. Gereç ve Yöntem: Hastanemizde akromegali nedeni ile opere edilen 110 hastanın verileri geriye dönük olarak tarandı. Kırk üç hastanın tetkikleri eksik olduğundan çalışma dışı bırakıldı. Tüm hastalar klinik ve laboratuvar olarak operasyon öncesi değerlendirilmiştir. Bulgular: Hastaların 42'si kadın, 25'i erkekti. Otuz hastada sadece büyüme hormonu boyanması varken kalan 37 hastada plurihormonal boyanma saptandı. Tiroid uyarıcı hormon (TSH) boyanması olan 14 hastanın üçüne preoperatif olarak TSH salgılayan hipofiz adenomları tanısı konmuştu. Bu üç olgu dışında klinik ve laboratuvar olarak hormon sekrete eden adenom saptanmadı. Tartışma: Çalışmamız akromegali hastalarında immünohistokimyasal olarak saptanan boyanma paternleri ile klinik ve laboratuvar bulguları arasında ilişki olmadığını gösterdi.

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Section: Discussioncontrasting

confidence: 40%

Section: Introductionmentioning

confidence: 99%

Immunostaining Results of Growth Hormone Secreting Adenomas and Their Correlation with Laboratory Findings

Tuna¹,

Karakılıç²,

Basaran³

et al. 2016

tjem

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show abstract

“…Fivemicrometre thick serial sections were stained with the routine haematoxylin and eosin method for the histopathology evaluation, and IHC was performed via the avidin-biotin-peroxidase method for PRL, GH, ACTH, FSH, LH, and TSH, as previously described by our group (26,27). For some of the cases, immunohistochemistry for Ki-67 was performed.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

The Efficacy of Early Postoperative Radiotherapy for Non-Functioning Pituitary Macro Adenomas, with Tumour Cells Expressing or not Expressing Pituitary Hormones

Stancu¹

2014

Acta Endo (Buc)

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Background. Radiotherapy after surgery for non-functioning pituitary macroadenomas (NFMAs) is still under debate.Aim. To appreciate the best timing for postoperative high voltage radiotherapy (hRT) in different type of NFMAs (classified using immunohistochemistry (IHC)).Subjects and Methods. Of 97 patients with a remnant (>1 cm) and IHC for anterior pituitary hormones, 41 patients (groups A & B) were submitted to hRT and followed up at least 5 years. RT was performed in 20/41 patients (Group A) within the first year after surgery, 21/41 patients (Group B) afterwards while in control group C, 56 patients were followed up without hRT. The progression of postoperative remnant was defined as a change of minimum of 25% of any diameter (transversal or vertical) by serial imaging studies.Results. The IHC of NFMAs revealed the following: 38 (39%) null cell, 29 (30%) gonadotropinomas, 12 (12%) silent plurihormonal, 11 (11%) silent corticotroph and 7 (7%) silent GH/PRL adenomas.Immunoreactive adenomas have relapse rate higher than null cell adenomas (ACTH> GH/ PRL> FSH/ LH> null cell) with a significant rate for silent ACTH 6/8 (75%). The null cell adenoma relapse rate was 6/23 (26%), p<0.009 in group without hRT. The relapse rate was significantly lower in group AB with hRT than in group without RT (p=0.025), at five years. Immediate hRT (applied within in first years) improved the control of the tumour growth in 90% (18/20) cases. In the group A, 10 % (2/20) patients relapsed than 24% (5/21) patients in group B and 39% (22/56) patients, Group C.Conclusion. An optimal time for radiotherapy is within the first year after the partial surgical removal of NFMAs, particularly if a large amount of residual tumour remains. Patients with silent corticotroph adenoma require special attention.

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“…However limits and controversies around the diagnosis of pituitary adenomas were reported also using more sophisticated techniques. Discrepancies between immunohistochemistry and electronmicroscopy have also been described (20).…”

Section: Discussionmentioning

confidence: 96%

Reticular fibers network discriminates between normal hypophysis and pituitary adenomas

Ceauşu¹

2010

Acta Endo (Buc)

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Background. Usually, silver stain is needed to differentiate between normal or hyperplastic hypophysis and pituitary adenomas. Many papers reported the lack of reticular fibers network as mandatory for pituitary adenoma diagnosis.Aim. Differences between the architecture of reticular fibers in normal pituitary and pituitary adenomas.Methods. Gordon-Sweet silver staining of pituitary specimens, prelevated during pituitary surgery performed in 138 patients with the endocrine and imagistic diagnosis of pituitary macro-adenomas.Results. Pituitary specimens of pituitary adenomas was confirmed in 133 cases; 3 specimens were with normal pituitary tissue, 1 with pituitary hyperplasia, 1 with pituitary apoplexy. Twelve of 133 pituitary adenomas specimens were associated also with normal pituitary tissue. There was a loss of acinar network of reticular fibres in 115 cases, but the present study describes the persistence of reticular fibers networks in 18 (13.53%) of 133 pituitary adenomas. We identified five distribution patterns of remanent reticular fibers network in pituitary adenomas using silver staining.Conclusion. Persistence of reticular fibers in a small number of pituitary adenomas could be a possible pitfall in the discrimination between the normal hypophysis and pituitary adenoma.

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"Mind the gap" in immunohistochemical techniques used in pathology of pituitary adenomas

Cited by 6 publications

References 17 publications

Immunostaining Results of Growth Hormone Secreting Adenomas and Their Correlation with Laboratory Findings

Immunostaining Results of Growth Hormone Secreting Adenomas and Their Correlation with Laboratory Findings

The Efficacy of Early Postoperative Radiotherapy for Non-Functioning Pituitary Macro Adenomas, with Tumour Cells Expressing or not Expressing Pituitary Hormones

Reticular fibers network discriminates between normal hypophysis and pituitary adenomas

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