Microwave assisted synthesis of tyrosine protected gold nanoparticles for dual (colorimetric and fluorimetric) detection of spermine and spermidine in biological samples

Rawat, Karuna A.; Bhamore, Jigna R.; Singhal, R. K.; Kailasa, Suresh Kumar

doi:10.1016/j.bios.2016.07.069

Cited by 77 publications

(40 citation statements)

References 45 publications

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“…During the next several hours (3-12 h), AuNCs become more ordered inside the protein due to structural optimization [32]. Furthermore, it is known that tyrosine and tryptophan are able to reduce Au(III) under strongly alkaline pH values to Au(0), thus forming AuNCs and nanoparticles [42][43][44]. Therefore, cysteine, tyrosine, and tryptophan residues are visualized in Figure 1.…”

Section: Bsa Structural Changes In the Course Of Auncs Synthesismentioning

confidence: 99%

The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

et al. 2020

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Fluorescent gold nanoclusters (AuNCs) are envisaged as a novel type of fluorophores. This work reports on the first comparative study investigating the effect of presence/absence/abundance of fatty acids (namely palmitic acid, PA) or other substances (like glycoproteins and globulins) in the protein (bovine serum albumin, BSA) on synthesis and properties of the final AuNCs. The most popular template (BSA) and microwave (MW)-assisted synthesis of AuNCs have been intentionally chosen. Our results clearly demonstrate that the fluorescent characteristics (i.e., fluorescence lifetime and quantum yield) are affected by the fatty acids and/or other substances. Importantly, the as-prepared AuNCs are biocompatible, as determined by Alamar Blue assay performed on Hep G2 cell line.

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Section: Bsa Structural Changes In the Course Of Auncs Synthesismentioning

confidence: 99%

The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

et al. 2020

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“…Through their intrinsic advantages, including simple and low-cost fabrication process, compatibility with other state-of-the-art technologies such as printed circuit boards, complementary metal oxides semiconductors (CMOS), and microfluidic lab-on-a-chip systems 38 , their merits increase significantly as biosensors in biomedical applications. Several groups including ours have integrated planar microwave sensing structures with microfluidic technology to demonstrate outstanding potential for sensing and monitoring 36 , 37 , 39 , where the resonator is able to detect changes in the electric field as a result of changes in liquid inside the microfluidic channel. Nicolic-Jarik et al .…”

Section: Introductionmentioning

confidence: 99%

Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor

Narang

Mohammadi

et al. 2018

Sci Rep

127

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Infection diagnosis and antibiotic susceptibility testing (AST) are time-consuming and often laborious clinical practices. This paper presents a microwave-microfluidic biosensor for rapid, contactless and non-invasive device for testing the concentration and growth of Escherichia Coli (E. Coli) in medium solutions of different pH to increase the efficacy of clinical microbiology practices. The thin layer interface between the microfluidic channel and the microwave resonator significantly enhanced the detection sensitivity. The microfluidic chip, fabricated using standard soft lithography, was injected with bacterial samples and incorporated with a microwave microstrip ring resonator sensor with an operation frequency of 2.5 GHz and initial quality factor of 83 for detecting the concentration and growth of bacteria. The resonator had a coupling gap area on of 1.5 × 1.5 mm2 as of its sensitive region. The presence of different concentrations of bacteria in different pH solutions were detected via screening the changes in resonant amplitude and frequency responses of the microwave system. The sensor device demonstrated near immediate response to changes in the concentration of bacteria and maximum sensitivity of 3.4 MHz compared to a logarithm value of bacteria concentration. The minimum prepared optical transparency of bacteria was tested at an OD600 value of 0.003. The sensor’s resonant frequency and amplitude parameters were utilized to monitor bacteria growth during a 500-minute time frame, which demonstrated a stable response with respect to detecting the bacterial proliferation. A highly linear response was demonstrated for detecting bacteria concentration at various pH values. The growth of bacteria analyzed over the resonator showed an exponential growth curve with respect to time and concurred with the lag-log-stationary-death model of cell growth. This biosensor is one step forward to automate the complex AST workflow of clinical microbiology laboratories for rapid and automated detection of bacteria as well as screening the bacteria proliferation in response to antibiotics.

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“…Generally, AuNPs are chosen as an excellent platform for colorimetric detection. [26][27][28] The synthesized AuNPs in this work yield spherical nanoparticles with an average size of 13 nm and surface plasmon resonance (SPR) maximum around 520 nm. Since the AuNPs are capped with negatively charged citrate on the surface, 29 which can be conrmed by the zeta potential report (Fig.…”

Section: Resultsmentioning

confidence: 99%

Colorimetric sensing strategy for heparin assay based on PDDA-induced aggregation of gold nanoparticles

Kou

et al. 2019

Nanoscale Adv.

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Microwave assisted synthesis of tyrosine protected gold nanoparticles for dual (colorimetric and fluorimetric) detection of spermine and spermidine in biological samples

Cited by 77 publications

References 45 publications

The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor

Colorimetric sensing strategy for heparin assay based on PDDA-induced aggregation of gold nanoparticles

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