Microvessel Landscape Assessment in Pancreatic Ductal Adenocarcinoma

Lytras, Dimitrios; Leontara, Vassilia; Kefala, Maria; Foukas, Periklis; Giannakou, Niki; Pouliakis, Abraham; Dervenis, Christos; Panayiotides, Ioannis G.; Karakitsos, Petros

doi:10.1097/mpa.0000000000000197

Cited by 9 publications

(3 citation statements)

References 44 publications

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“…Pancreatic ductal adenocarcinoma is a hypovascular and a hypoxic tumor. [25][26][27] This is confirmed in this study, since both the benign and malignant sites showed very low levels of mean blood volume (1.1% and 2.1%, respectively). These results are supported by a study of Erkan et al, 25 in which the microvascular density was determined: normal pancreas showed a five times higher microvascular density than fibrotic areas of chronic pancreatitis, and four times higher than pancreatic ductal adenocarcinoma.…”

Section: Discussionsupporting

confidence: 81%

Toward optical guidance during endoscopic ultrasound-guided fine needle aspirations of pancreatic masses using single fiber reflectance spectroscopy: a feasibility study

et al. 2017

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Endoscopic ultrasound-guided fine needle aspirations (EUS-FNA) of pancreatic masses suffer from sample errors and low-negative predictive values. Fiber-optic spectroscopy in the visible to near-infrared wavelength spectrum can noninvasively extract physiological parameters from tissue and has the potential to guide the sampling process and reduce sample errors. We assessed the feasibility of single fiber (SF) reflectance spectroscopy measurements during EUS-FNA of pancreatic masses and its ability to distinguish benign from malignant pancreatic tissue. A single optical fiber was placed inside a 19-gauge biopsy needle during EUS-FNA and at least three reflectance measurements were taken prior to FNA. Spectroscopy measurements did not cause any related adverse events and prolonged procedure time with ? 5 ?? min . An accurate correlation between spectroscopy measurements and cytology could be made in nine patients (three benign and six malignant). The oxygen saturation and bilirubin concentration were significantly higher in benign tissue compared with malignant tissue (55% versus 21%, p = 0.038 ; 166 ?? ? mol / L versus 17 ?? ? mol / L , p = 0.039 , respectively). To conclude, incorporation of SF spectroscopy during EUS-FNA was feasible, safe, and relatively quick to perform. The optical properties of benign and malignant pancreatic tissue are different, implying that SF spectroscopy can potentially guide the FNA sampling.

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Section: Discussionsupporting

confidence: 81%

Toward optical guidance during endoscopic ultrasound-guided fine needle aspirations of pancreatic masses using single fiber reflectance spectroscopy: a feasibility study

et al. 2017

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“…It is notably upregulated in endothelial cells and has been considered to be a biomarker for tumor angiogenesis ( 12 ). The role of CD105 in the early metastasis of a tumor has gained attention, including in squamous cell carcinoma and pancreatic ductal adenocarcinoma ( 13 , 14 ). In RCC, the function of CD105 remains controversial ( 15 , 16 ).…”

Section: Introductionmentioning

confidence: 99%

Expression of CK19, CD105 and CD146 are associated with early metastasis in patients with renal cell carcinoma

et al. 2018

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The present study was designed in order to explore the association between the early metastasis of renal cell carcinoma (RCC) and biological markers of tumor cells. A total of 200 patients with RCC, who received a nephrectomy between January 2015 and October 2015, were enrolled in the present study, while 100 healthy patients served as controls. The expression of cytokeratin 19 (CK19), endoglin (CD105) and cluster of differentiation 146 (CD146) were detected using immunohistochemical staining and western blotting. All three markers were highly expressed in tumor tissues compared with adjacent normal tissues. Subsequently, an enzyme-linked immunosorbent assay was used to detect the differential expression of CK19, CD105 and CD146. The results revealed that there was a statistically significant difference in the expression of CK19 and CD105 between the two groups (P<0.05), whereas CD146 did not exhibit a statistically significant difference. The results of further experiments revealed no significant difference between four time points (Q1, 1 day pre-operation; Q2, 1 day post-operation; Q3, 1 week post-operation; and Q4, 1 month post-operation). Then, subgroup analysis was performed based on whether patients were circulating tumor cell (CTC)-positive or not, and the difference between the Q1 time point and other three time points (Q2-4). The results revealed no difference between the CTC-positive and -negative groups, and no difference between the time points Q1 and Q2. However, the expression of CK19 and CD105 exhibited a significant difference between CTC-positive and CTC-negative groups according to the difference between the time points Q1 and Q3. Furthermore, on the basis of the difference between Q1 and Q4, the expression of CK19, CD105 and CD146 were significantly different (P<0.05). Taken together, the results suggested that CK19, CD105 and CD146 markers of peripheral blood may be considered to be effective tools to evaluate the early metastasis in a CTC-positive condition. CK19, CD105 and CD146 may be useful for CTC in evaluating the prognosis of patients with RCC, although a larger sample size is necessary for further investigation.

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“…Microcirculatory maladjustment may lead to inadequate nutrients and oxygen supply, which results in consequential pancreatic dysfunction. 1 Emerging evidence confirms that pancreatic microcirculation profiles may serve as a determinant for variety of pathological conditions such as diabetes, 2 acute pancreatitis, 3 , 4 pancreatic cancer, 5 , 6 even hypertension, and obesity. 7 Therefore, the pancreatic microcirculation may herald, either as a marker or as a possible mechanism, the development of pancreatic-related diseases.…”

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confidence: 98%

Multimodal Device and Computer Algorithm–Based Monitoring of Pancreatic Microcirculation Profiles In Vivo

Song

Liu³

et al. 2020

Pancreas

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Objectives Pancreatic microcirculation has an essential role in orchestrating pancreatic homeostasis. Inherent complexity and technological limitation lead to interobserver variability and 1-sided microcirculatory data. Here, we introduce a multimodal device and computer algorithm–based platform for monitoring and visualizing integrated pancreatic microcirculation profiles. Methods After anesthetizing and exposing pancreas tissue of BALB/c mice, probes of Oxygen to See, Microx TX3, and MoorVMS-LDF2 were positioned at pancreas in situ to capture the pancreatic microcirculatory oxygen (hemoglobin oxygen saturation, relative amount of hemoglobin, and partial oxygen pressure) and microhemodynamic data (microvascular blood perfusion and velocity). To assess and visualize pancreatic microcirculation profiles, raw data of pancreatic microcirculation profiles were processed and transformed using interquartile range and min-max normalization by Python and Apache ECharts. Results The multimodal device–based platform was established and 3-dimensional microcirculatory modules were constructed. Raw data sets of pancreatic microcirculatory oxygen and microhemodynamic were collected. The outlier of data set was adjusted to the boundary value and raw data set was preprocessed. Normalized pancreatic microcirculation profiles were integrated into the 3-dimensional histogram and scatter modules, respectively. The 3-dimensional modules of pancreatic microcirculation profiles were then generated. Conclusions We established a multimodal device and computer algorithm–based monitoring platform for visualizing integrated pancreatic microcirculation profiles.

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Microvessel Landscape Assessment in Pancreatic Ductal Adenocarcinoma

Abstract: Further studies are needed before endoglin replaces the conventional angiogenesis markers in PDCA.

Cited by 9 publications

References 44 publications

Toward optical guidance during endoscopic ultrasound-guided fine needle aspirations of pancreatic masses using single fiber reflectance spectroscopy: a feasibility study

Toward optical guidance during endoscopic ultrasound-guided fine needle aspirations of pancreatic masses using single fiber reflectance spectroscopy: a feasibility study

Expression of CK19, CD105 and CD146 are associated with early metastasis in patients with renal cell carcinoma

Multimodal Device and Computer Algorithm–Based Monitoring of Pancreatic Microcirculation Profiles In Vivo

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