Expression of CK19, CD105 and CD146 are associated with early metastasis in patients with renal cell carcinoma

Yang, Xiaojie; Zhang, Dong; Chong, Tie; Li, Youfang; Wang, Ziming; Zhang, Peng

doi:10.3892/ol.2018.7871

Cited by 10 publications

(13 citation statements)

References 34 publications

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“…It was revealed that the high CD105 copy number group survived for longer and this trend was in accordance with the in the high CD105 mRNA group. This appears in contrast with previous experimental results, where higher CD105 expression indicated more invasion and poor prognosis (20). Of note, previous studies also suggested that CD105 is an independent predictive marker for the risk of death and unfavourable prognosis in patients with ccRCC after curative resection (21,22).…”

Section: Discussioncontrasting

confidence: 99%

Expression and clinical value of CD105 in renal cell carcinoma based on data mining in The Cancer Genome Atlas

et al. 2019

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The objective of the present study was to assess the expression of CD105 and its association with overall survival in three subtypes of renal cell carcinoma (RCC), namely clear cell (cc)RCC, papillary (p)RCC and chromophobe (ch)RCC. Data regarding the transcriptome and copy number of genes in RCC tumor samples and survival were obtained from The Cancer Genome Atlas. Bioinformatics analysis revealed that CD105 is overexpressed in ccRCC tumor tissue vs. normal renal tissue, and a higher CD105 copy number in ccRCC tissues was significantly associated with longer patient survival. The effect of the mRNA expression of CD105 in all three types of RCC and the copy number in pRCC and chRCC on patient survival was insignificant, but certain trends were observed. In addition, CD105 mRNA expression was associated with the metastasis and tumor stage, as well as pathological stage in ccRCC and pRCC. Pathway enrichment analysis revealed that CD105 may, through translation initiation of associated genes, promote RCC progression. The results of the present study suggest that in RCC tumors, the association of CD105 with different stages is complex. To evaluate the role of CD105 in RCC, its function should be assessed in addition to its expression. The exact influence of CD105 mRNA expression and copy number in RCC tumors on patient survival and the underlying mechanisms require further elucidation.

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Section: Discussioncontrasting

confidence: 99%

Expression and clinical value of CD105 in renal cell carcinoma based on data mining in The Cancer Genome Atlas

et al. 2019

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“…Jakobiec et al (14) applied CK15 expression to investigate the property of the adenoma sebaceum, and Andruszkow et al (15) utilized CK19 expression for differential diagnosis of the basal cell carcinoma and adenoma sebaceum. Furthermore, it is reported that CK expression levels can be used to diagnose clear cell hidroadenoma (16) and primary neuroendocrine carcinoma (17) clinically, suggesting that detection of CKs may be beneficial to diagnosing epithelial tumor of the skin. In this study, comprehensive analyses on the experimental results were performed by virtue of the semi-quantitative scoring method via immunohistochemistry, and the findings were in line with partial results of previous studies.…”

Section: Discussionmentioning

confidence: 99%

Cytokeratin expression in epidermal stem cells in skin adnexal tumors

Xiao-fang

Wang

et al. 2018

Oncol Lett

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The expression levels of seven types of cytokeratin (CK) in different kinds of skin adnexal tumors were evaluated. One hundred and thirty-two patients with different kinds of skin adnexal tumors admitted and treated in the Department of Dermatology of Dongying People's Hospital from May 2013 to May 2015 were selected and underwent tissue section staining. Another 20 cases of normal skin were enrolled as the control group. The expression levels of the seven types of CK in different kinds of skin appendages were observed and recorded. The expression levels of the seven types of CK in the 132 cases of skin adnexal tumor tissues were different. CK10 was mainly expressed in squamous cell carcinoma, but it was not expressed in basal cell carcinoma. CK19 was expressed in basal cell carcinoma, but its expression was not detected in squamous cell carcinoma. As the degree of differentiation was increased in the epidermis, hair follicle and sebaceous gland, the expressed molecular weight of CK was augmented gradually. The expression levels of five types of CK (namely, CK8, CK10, CK14, CK18 and CK19) could be measured in the squamous cell carcinoma and basal cell carcinoma. Statistical analysis revealed that there were statistically significant differences in the expression levels of these five types of CK in the two cell carcinomas (P<0.05). Five types of CK, i.e., CK7, CK8, CK17, CK18 and CK19, had markedly different expression levels in hair follicle tumor and sweat gland tumor, which were statistically significant (P<0.05). The expression levels of a group of CKs detected by virtue of semi-quantitative analysis via immunohistochemistry can be regarded as one of the important indexes for clinical diagnosis of skin adnexal tumors.

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“…In RCC cell lines CD105 www.nature.com/scientificreports www.nature.com/scientificreports/ was reported by us to be highly expressed in metastatic Caki-1 cell line before 12 , as well as by other groups 28 . Moreover, detection of CD105 marker in peripheral blood is considered to be effective tool to evaluate the early metastasis in RCC, especially in a cancer stem cell -positive conditions 30 . CD105 was shown to play functional role in maintaining cancer stem cell phenotype and epithelial -mesenchymal transition (EMT) phenotype 31 , while cells after EMT disseminate to distant organs and form metastases 32 .…”

Section: Discussionmentioning

confidence: 99%

Renal carcinoma CD105−/CD44− cells display stem-like properties in vitro and form aggressive tumors in vivo

Fiedorowicz

Khan

Strzemecki

et al. 2020

Sci Rep

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clear cell renal cell carcinoma (ccRcc) is the most common kidney cancer. prognosis for ccRcc is generally poor since it is largely resistant to chemo-and radiotherapy. Many studies suggested that cancer stem cells/tumor initiating cells (CSCs/TICs) are responsible for development of tumor, disease progression, aggressiveness, metastasis and drug resistance. However, tumorigenic potential of CSCs/ tics isolated from established Rcc cell lines-basic ccRcc research model-has never been investigated in vivo. CD105+, CD105−, CD44+ and CD44− as well as CD44−/CD105− CD44+/CD105+ and CD44−/ CD105+ cells were isolated from Caki-1 RCC cell line, confirming coexistence of multiple subpopulations of stem-related phenotype in stable cell line. Sorted cells were injected subcutaneously into noD SciD mice and tumor growth was monitored with MRi and pet/ct. tumor growth was observed after implantation of CD105+, CD44+, CD44−, CD44−/CD105+ and CD44−/CD105− but not CD105− or CD44+/CD105+. Implantation of CD44−/CD105− cells induced tumors that were characterized by longer T1 and distinct metabolic pattern than other tumors. All the tumors were characterized by low uptake of [18F]FDG. CD105+ and CD44− tumors expresses Nanog and Oct-4, while CD44− tumors additionally expressed endothelial cell marker-CD31. Renal cell carcinoma (RCC), is the 10th malignancy worldwide and the most frequent type of kidney cancer in adults. Each year in Europe approximately 88 400 patients are diagnosed with RCC; the incidence and mortality of RCC are rising at a rate of 2-3% per decade, therefore novel therapies directed against RCC are needed. At the same time despite advancements in diagnostic techniques, up to 30% of newly diagnosed patients already present with metastases, and a large portion of patients that undergo surgical treatment experience the RCC recurrence, therefore drugs targeted against metastasis initiating cells would be of great interest in the future 1,2. Cancer stem cells (CSCs) are characterized by the potential to self-renew, high tumorigenicity in nude mice and the ability to efficiently reconstitute all tumor subpopulations and primary tumor phenotype 3-5. CSCs are also responsible not only for cancer development, but also for disease recurrence, progression and metastatic spread, together with cancer aggressiveness, including treatment resistance such as chemo/radiotherapy, and targeted treatment 6,7 , therefore basic research with careful model selection to understand their biology is mandatory to define novel potential therapeutic targets for all RCC subtypes 8,9 .

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Expression of CK19, CD105 and CD146 are associated with early metastasis in patients with renal cell carcinoma

Cited by 10 publications

References 34 publications

Expression and clinical value of CD105 in renal cell carcinoma based on data mining in The Cancer Genome Atlas

Expression and clinical value of CD105 in renal cell carcinoma based on data mining in The Cancer Genome Atlas

Cytokeratin expression in epidermal stem cells in skin adnexal tumors

Renal carcinoma CD105−/CD44− cells display stem-like properties in vitro and form aggressive tumors in vivo

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