Microvessel density, mast cell density and thymidine phosphorylase expression in oral squamous carcinoma

Ranieri, Girolamo; Labriola, A; Achille, Gaetano; Florio, Gerolmina; Zito, Alfredo; Grammatica, Luciano; Paradiso, A

doi:10.3892/ijo.21.6.1317

Cited by 56 publications

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“…Tumour tissue angiogenesis is often evaluated by determining microvascular density (MVD) and endothelial area (EA) (1)(2)(3). MVD and EA have been experimentally proposed to be biomarkers associated with biological aggressiveness and clinical outcome in animal and human malignancies.…”

Section: Introductionmentioning

confidence: 99%

“…However, limited data exists regarding the association between tumour cell proliferation and angiogenesis in primary tumour tissue from pancreatic ductal adenocarcinoma (PDAC) patients; therefore, the present study aimed to investigate this association. A series of 31 PDAC patients with stage Tumour (T) [2][3] Node (N) 0-1 Metastasis (M) 0 were recruited into the present study and subsequently underwent surgery. PDAC tissue and adjacent normal tissue (ANT), resected during surgery, were evaluated using immunohistochemistry and image analysis methods to determine MVD, endothelial area (EA) and Ki-67 expression, which is an indicator of cell proliferation rate.…”

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confidence: 99%

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Microvascular density and endothelial area correlate with Ki-67 proliferative index in surgically-treated pancreatic ductal adenocarcinoma patients

et al. 2015

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Abstract. Previous experimental and clinical data have indicated that tumour cell proliferation is associated with angiogenesis; in addition, an increased microvascular density (MVD) of tumours has been associated with poor prognosis in solid and haematological malignancies. However, limited data exists regarding the association between tumour cell proliferation and angiogenesis in primary tumour tissue from pancreatic ductal adenocarcinoma (PDAC) patients; therefore, the present study aimed to investigate this association. A series of 31 PDAC patients with stage Tumour (T) [2][3] Node (N) 0-1 Metastasis (M) 0 were recruited into the present study and subsequently underwent surgery. PDAC tissue and adjacent normal tissue (ANT), resected during surgery, were evaluated using immunohistochemistry and image analysis methods to determine MVD, endothelial area (EA) and Ki-67 expression, which is an indicator of cell proliferation rate. The results demonstrated a correlation between the above parameters with each other as well as the main clinico-pathological features of PDAC. Significant differences were identified in MVD, EA and Ki-67 proliferation index between PDAC and ANT. It was demonstrated that MVD, EA and Ki-67 proliferation index were significantly correlated with each other in tumour tissue (r=0.69-0.81; P=0.001-0.003). However, no other significant correlations were identified. These data therefore suggested that angiogenesis and cell proliferation rate were significantly increased in PDAC compared with ANT, which provides a biological basis for the potential use of novel combinations of angiogenesis inhibitors and anti-proliferative chemotherapeutic drugs in the treatment of PDAC.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Microvascular density and endothelial area correlate with Ki-67 proliferative index in surgically-treated pancreatic ductal adenocarcinoma patients

et al. 2015

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“…Angiogenesis plays a crucial role in hematogenous and lymphatic metastases for which studies have suggested that lesions that entered a higher angiogenic state have an increased probability of metastasis (3,4). However, certain tumors with marked angiogenesis surprisingly had no evidence of metastasis and were associated with a good prognosis (5,6). Thus, an unclear relationship exists among angiogenesis, metastasis and prognosis.…”

Section: Introductionmentioning

confidence: 97%

“…Also, lymphatic vessels are the preferential routes for metastatic spread of most carcinomas that arise in the cervix (8). Distant lymph node metastasis of cancer cells is often due to the involvement of lymphatic vessels (4,5). Despite the occurrence of lymphatic metastasis in those tumors, few studies have focused on the distribution and characteristics of lymphatic vessels in cancers (9,10).…”

Section: Introductionmentioning

confidence: 99%

Localization and characterization of lymphatic vessels in oral and cervical squamous cell carcinoma

Inoue

Roan

Abe

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. Lymph node metastasis is considered a factor in determining the prognosis of squamous cell carcinoma (SCC). Both oral and cervical SCC tumor cells prefer lymph vessels as the route of metastasis. D2-40 is a specific marker of lymphatic endothelial cells. This study clarifies the distribution and characteristics of lymphatic vessels in oral and cervical SCCs. Immunohistochemistry was performed in 20 oral and 20 cervical SCCs (10 non-metastatic and 10 metastatic to lymph nodes) using D2-40, CD31, CD34, CD105 and double staining with D2-40 and keratin. Lymphatic vessel density (LVD) was also determined morphologically. Results showed that lymphatic vessels in both types of SCCs were distributed mainly at the superficial region beneath the epithelium. The LVD in each tumor was significantly higher compared to the corresponding normal mucosa. Moreover, the LVD in lymph node metastasis in each tumor was significantly higher compared to their non-metastatic counterparts. Cancer cell invasion was observed in the lymphatic vessels suggesting the existence of lymph node involvement during metastasis. The new lymphatic vessels that proliferated around the cancer nests in both SCCs have endothelial cell characteristics inferred to be associated with early lymphatic development and initial dissemination of cancer cells. IntroductionThe role of blood vessels in tumor progression and metastasis has been reported in various malignancies, including oral and cervical cancers (1-3). However, the relationship between blood vessels and lymph node metastasis in malignant tumors is largely unknown. Angiogenesis plays a crucial role in hematogenous and lymphatic metastases for which studies have suggested that lesions that entered a higher angiogenic state have an increased probability of metastasis (3,4). However, certain tumors with marked angiogenesis surprisingly had no evidence of metastasis and were associated with a good prognosis (5,6). Thus, an unclear relationship exists among angiogenesis, metastasis and prognosis.Clinically, lymph node metastasis in oral and cervical cancers occurs in 20-30% of cases and is considered a major factor in determining prognosis. Lymphatic spread is more important than other routes. Via this route malignant cells preferentially metastasize in lymph nodes in the cervical region (7). Also, lymphatic vessels are the preferential routes for metastatic spread of most carcinomas that arise in the cervix (8). Distant lymph node metastasis of cancer cells is often due to the involvement of lymphatic vessels (4,5). Despite the occurrence of lymphatic metastasis in those tumors, few studies have focused on the distribution and characteristics of lymphatic vessels in cancers (9,10). Moreover, it remains questionable whether tumor cells induce lymphangiogenesis.Several lymphatic-specific antibodies have been developed (11). Kahn and Marks developed an antibody called D2-40, reported to be effective in detecting lymphatic vessels in formalin-fixed paraffin sections (12). The antibody does not requi...

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“…12 Some studies have shown a significant correlation between mast cell density and microvessel density in preneoplastic lesions and in oral SCC 12,13 and have suggested that mast cells may increase angiogenesis in these tumors via tryptase.…”

Section: 3mentioning

confidence: 99%

Comparação entre a concentração de mastócitos em carcinomas espinocelulares da pele e da cavidade oral

Parizi¹,

Barbosa

Parizi

et al. 2010

An. Bras. Dermatol.

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FUNDAMENTS:The lethality of squamous cell carcinomas (SCC) of the skin is considered low. SCC in the mouth is usually associated with poor prognosis. Current evidence suggests that mast cells in the normal tissue contribute to the tumorigenesis of SCC, probably by promoting angiogenesis. OBJECTIVE: The aim of this study was to compare the concentration of mast cells in SCC of the mouth and skin and evaluate whether there is a correlation with the degree of differentiation of these tumors. MATERIAL AND METHODS: Thirty cases of SCC of the skin and 34 of the mouth were investigated. Toluidine blue staining was used to identify mast cells in blocks containing the central portion of the neoplasm. RESULTS: A concentration of between 0 and 10 mast cells was found in one single case of SCC of the skin and there were no cases of SCC of the mouth with concentrations of mast cells in the tumor >201. In the majority of cases of SCC of the mouth (47%; n=16), mast cell concentration was between 0 and 10, with a concentration >51 mast cells in 80% of cases of SCC of the skin. All the cases of SCC of the mouth with a concentration of mast cells between 100 and 200 and 80% of those with a concentration of 51-99 were located on the lip. The concentration of mast cells was unrelated to the degree of differentiation of the tumor. CONCLUSION: The concentration of mast cells is lower in SCC of the mouth except when the tumor is located on the lip. This may reflect a lower need for cell activation in the microenvironment to improve vascularization in oral cancer.

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Microvessel density, mast cell density and thymidine phosphorylase expression in oral squamous carcinoma

Cited by 56 publications

References 0 publications

Microvascular density and endothelial area correlate with Ki-67 proliferative index in surgically-treated pancreatic ductal adenocarcinoma patients

Microvascular density and endothelial area correlate with Ki-67 proliferative index in surgically-treated pancreatic ductal adenocarcinoma patients

Localization and characterization of lymphatic vessels in oral and cervical squamous cell carcinoma

Comparação entre a concentração de mastócitos em carcinomas espinocelulares da pele e da cavidade oral

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