Microvascular maturity elicited in tissue treated with cytokine-loaded hyaluronan-based hydrogels

Abstract

Hydrogels composed of crosslinked, chemically modified hyaluronic acid (HA), gelatin (Gtn) and heparin (Hp) were preloaded with vascular endothelial growth factor (VEGF), angiopoietin-1

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

“…Several studies have focused on a controlled delivery of growth factors over a longer duration of time in order to simulate the natural sequestration of growth factors in native body. VEGF and Ang-1 were delivered in a hydrogel to elicit vascular maturity in rat aortic rings [43]. Mooney and colleagues have devised several strategies to deliver multiple growth factors through controlled release either by incorporating the growth factors directly or seeding endothelial cells into polymeric scaffolds that in turn release the growth factors in a controlled fashion.…”

Ophthalmic Uses of a Thiol-Modified Hyaluronan-Based Hydrogel

“…For example, wound healing was accelerated in a diabetic mouse model when basic fibroblast growth factor was continuously released from the CMHA-S hydrogels into full-thickness wounds. 23,40 Other proteins and small molecules that have been delivered both in vitro and in vivo from these CMHA-S gels and films include the following growth factors: 28,[41][42][43][44][45] Basic…”

Hydrogels for therapeutic cardiovascular angiogenesis

“…These microvessels organized into recognizable tubular networks filled with erythrocytes [33]. Inclusion of heparin by covalently binding it with HA-based hydrogel proved to be more optimized in providing a greater angiogenic response [31,32,34]. Heparin is a highly sulfated GAG known for its affinity for a variety of angiogenic growth factors and ability to sequester those growth factors in the ECM.…”

“…While the high molecular weight HA has inhibitory angiogenic effects, the low ones stimulate endothelial cell proliferation and migration [28][29][30]. Chemically modified forms of HA have been developed to form biocompatible, macroporous hydrogel to store and release growth factors in vivo [31][32][33][34]. HA hydrogels carrying both VEGF and keratinocyte growth factor (KGF) upon transplantation into ear pinnae of mice generated intact microvessel beds with well-defined borders.…”