Hydrogels for therapeutic cardiovascular angiogenesis

Rufaihah, Abdul Jalil; Seliktar, Dror

doi:10.1016/j.addr.2015.07.003

Cited by 82 publications

(68 citation statements)

References 95 publications

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“…53 The process of ALG sulfation was characterized by 13 C NMR and FTIR. Lack of chemical shift of C1 and C6 in 13 C NMR spectra means that sulfate groups were not added to those carbons and were mainly added to the OH groups (either one of the C2 or C3 or both) of uronic acid. The shift of the carbons C2 and C3 to lower field position was due to the direct attachment of electronegative sulfate ester groups, while the shift of C4 and C5 to higher field positions was a result of indirect attachment to sulfate ester groups, as shown previously by others.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, ALG is an attractive material for use in sustained delivery of bioactive molecules including drugs, proteins, and cells to wounds or damaged tissues . Despite these excellent properties, pure ALG has several disadvantages including uncontrollable degradation, limited range of mechanical properties and lack of receptors for cellular binding . The use of ALG for drug delivery applications has been limited by non‐specific ionic binding between the negatively charged ALG molecules and positively charged growth factors which lead to their uncontrolled release .…”

Section: Introductionmentioning

confidence: 99%

“…2 Despite these excellent properties, pure ALG has several disadvantages including uncontrollable degradation, limited range of mechanical properties and lack of receptors for cellular binding. 6,[12][13][14] The use of ALG for drug delivery applications has been limited by non-specific ionic Additional Supporting Information may be found in the online version of this article. Correspondence to: Prof. Mohsen Akbari e-mail: makbari@uvic.ca and Prof. Shahin Bonakdar e-mail: sh_bonakdar@pasteur.ac.ir Contract grant sponsor: Canada Foundation for Innovation binding between the negatively charged ALG molecules and positively charged growth factors which lead to their uncontrolled release.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Mohammadi

Ramakrishna

Laurent

et al. 2018

J Biomedical Materials Res

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Providing affinity sites on alginate (ALG) matrix enables specific binding of growth factors to the polymer backbone and allows their release in a controlled fashion. In this study, we used a blend of alginate sulfate (ALG‐S) and polyvinyl alcohol (PVA) to fabricate electrospun scaffolds capable of delivering a heparin‐like growth factor, transforming growth factor‐beta1 (TGF‐β1). The alginate was sulfated with different degrees of sulfation (DS, from 0.8, 3.4 to 12.4%) by a simple process. The success of sulfation was determined by Fourier‐transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), elemental analysis, ultraviolet (UV) spectroscopy and staining with dimethylmethylene blue. The physical‐mechanical properties of nanofibrous mats were characterized by scanning electron microscopy (SEM), FTIR, energy‐dispersive X‐ray spectroscopy (EDX), tensile strength and mass loss analysis. Additionally, the release kinetics of transforming growth factor‐β1 (TGF‐β1) from PVA/ALG‐S and PVA/ALG scaffolds were compared. The results showed that the binding and entrapment of TGF‐β1 to the nanofibrous scaffolds are improved by the addition of sulfate group to alginate. In conclusion, our results support that nanofibrous scaffold based on PVA/ALG‐S can deliver growth factors in tissue engineering application. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 403–413, 2019.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Mohammadi

Ramakrishna

Laurent

et al. 2018

J Biomedical Materials Res

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“…The angiogenic potential of hybrid scaffolds has been better studied using synthetic materials conjugated with unique combinations of tissue-derived angiogenic growth factors and must be precisely engineered for specific applications with engineered growth factor content and release profiles. [58] ECM bioscaffolds offer a distinct advantage over synthetic constructs in their tissue-specific mimicry through multi-factorial structural and signaling capacities. Furthermore, hydrogels derived from ECM bioscaffolds can be tailored for specific tissue regeneration applications through choice of source tissue, density and method of delivery.…”

Section: Discussionmentioning

confidence: 99%

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

et al. 2017

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Extracellular matrix (ECM)-derived bioscaffolds have been shown to elicit tissue repair through retention of bioactive signals. Given that the adventitia of large blood vessels is a richly vascularized microenvironment, we hypothesized that perivascular ECM contains bioactive signals that influence cells of blood vessel lineages. ECM bioscaffolds were derived from decellularized human and porcine aortic adventitia (hAdv and pAdv, respectively) and then shown have minimal DNA content and retain elastin and collagen proteins. Hydrogel formulations of hAdv and pAdv ECM bioscaffolds exhibited gelation kinetics similar to ECM hydrogels derived from porcine small intestinal submucosa (pSIS). hAdv and pAdv ECM hydrogels displayed thinner, less undulated, and fibrous microarchitecture reminiscent of native adventitia, with slight differences in ultrastructure visible in comparison to pSIS ECM hydrogels. Pepsin-digested pAdv and pSIS ECM bioscaffolds increased proliferation of human adventitia-derived endothelial cells and this effect was mediated in part by basic fibroblast growth factor (FGF2). Human endothelial cells cultured on Matrigel substrates formed more numerous and longer tube-like structures when supplemented with pAdv ECM bioscaffolds, and FGF2 mediated this matrix signaling. ECM bioscaffolds derived from pAdv promoted FGF2-dependent in vivo angiogenesis in the chick chorioallantoic membrane model. Using an angiogenesis-focused protein array, we detected 55 angiogenesis-related proteins, including FGF2 in hAdv, pAdv and pSIS ECMs. Interestingly, 19 of these factors were less abundant in ECMs bioscaffolds derived from aneurysmal specimens of human aorta when compared with nonaneurysmal (normal) specimens. This study reveals that Adv ECM hydrogels recapitulate matrix fiber microarchitecture of native adventitia, and retain angiogenesis-related actors and bioactive properties such as FGF2 signaling capable of influencing processes important for angiogenesis. This work supports the use of Adv ECM bioscaffolds for both discovery biology and potential translation towards microvascular regeneration in clinical applications.

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“…Gaetani et al reported that epicardial application of CPCs in a 3D-printed gelatin/hyaluronic acid patch supported the long-term cell engraftment, significantly reduced adverse remodeling and improved cardiac function [443]. At the same time, biomaterials can serve as carriers containing functional molecules that boost the regenrative capacity of SCs such as VEGF, bFGF, HGF, IGF-1 and TGF-β [444][445][446][447][448].…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

Müller

Lemcke

Dávid

2018

Cell Physiol Biochem

169

140

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A large number of clinical trials have shown stem cell therapy to be a promising therapeutic approach for the treatment of cardiovascular diseases. Since the first transplantation into human patients, several stem cell types have been applied in this field, including bone marrow derived stem cells, cardiac progenitors as well as embryonic stem cells and their derivatives. However, results obtained from clinical studies are inconsistent and stem cell-based improvement of heart performance and cardiac remodeling was found to be quite limited. In order to optimize stem cell efficiency, it is crucial to elucidate the underlying mechanisms mediating the beneficial effects of stem cell transplantation. Based on these mechanisms, researchers have developed different improvement strategies to boost the potency of stem cell repair and to generate the “next generation” of stem cell therapeutics. Moreover, since cardiovascular diseases are complex disorders including several disease patterns and pathologic mechanisms it may be difficult to provide a uniform therapeutic intervention for all subgroups of patients. Therefore, future strategies should aim at more personalized SC therapies in which individual disease parameters influence the selection of optimal cell type, dosage and delivery approach.

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Hydrogels for therapeutic cardiovascular angiogenesis

Cited by 82 publications

References 95 publications

Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

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