Ophthalmic Uses of a Thiol-Modified Hyaluronan-Based Hydrogel

Wirostko, Barbara; Mann, Brenda; Williams, David L.; Prestwich, Glenn D.

doi:10.1089/wound.2014.0572

Cited by 38 publications

(18 citation statements)

References 43 publications

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“…Thiolated HA (3,3‐dithiobis‐[propanoic dihydrazide]) or thiol‐carboxymethyl HA can be crosslinked to form a hydrogel by the addition of PEG diacrylate. These composites have been used as injectable scar‐free 3D cell scaffolds for wound healing or as 3D cell culture scaffolds . Cyto‐adhesiveness of the material can be increased by co‐crosslinking with gelatin, modified with thiol groups, yielding a gel with tripeptide Arg‐Gly‐Asp (RGD) motifs for binding integrins on cell surfaces .…”

Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

“…These composites have been used as injectable scar-free 3D cell scaffolds for wound healing or as 3D cell culture scaffolds. [247][248][249] Cyto-adhesiveness of the material can be increased by co-crosslinking with gelatin, modified with thiol groups, yielding a gel with tripeptide Arg-Gly-Asp (RGD) motifs for binding integrins on cell surfaces. 250 Moreover, HA can be chemically modified to acquire hydrophobic properties, which may be homogeneously mixed with gelatin to form hydrophobic-hydrophilic mixed gels used as 3D scaffolds for the chondrogenic differentiation of MSCs.…”

Section: Gelatin-hyaluronic Acidmentioning

confidence: 99%

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Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

292

210

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Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allow facile chemical modifications with other biomaterials or biomolecules. Despite the advantages of gelatin, poor mechanical properties, sensitivity to enzymatic degradation, high viscosity, and reduced solubility in concentrated aqueous media have limited its applications and encouraged the development of gelatin‐based composite hydrogels. The drawbacks of gelatin may be surmounted by synergistically combining it with a wide range of polysaccharides. The addition of polysaccharides to gelatin is advantageous in mimicking the ECM, which largely contains proteoglycans or glycoproteins. Moreover, gelatin–polysaccharide biomaterials benefit from mechanical resilience, high stability, low thermal expansion, improved hydrophilicity, biocompatibility, antimicrobial and anti‐inflammatory properties, and wound healing potential. Here, we discuss how combining gelatin and polysaccharides provides a promising approach for developing superior therapeutic biomaterials. We review gelatin–polysaccharides scaffolds and their applications in cell culture and tissue engineering, providing an outlook for the future of this family of biomaterials as advanced natural therapeutics.

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Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

Section: Gelatin-hyaluronic Acidmentioning

confidence: 99%

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

292

210

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“…26,28,29 Additionally, several PEG hydrogel-based medical devices composed of reactive PEG polymers including thiol-modified PEG and acrylate-modified PEG have received approval for use as sealants (CoSeal) 30 and wound healing matrices (Premvia). 31 …”

Section: Introductionmentioning

confidence: 99%

Liposome-Cross-Linked Hybrid Hydrogels for Glutathione-Triggered Delivery of Multiple Cargo Molecules

Liang¹,

Kiick

2016

Biomacromolecules

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Novel, liposome-cross-linked hybrid hydrogels cross-linked by the Michael-type addition of thiols with maleimides were prepared via the use of maleimide-functionalized liposome cross-linkers and thiolated polyethylene glycol (PEG) polymers. Gelation of the materials was confirmed by oscillatory rheology experiments. These hybrid hydrogels are rendered degradable upon exposure to thiol-containing molecules such as glutathione (GSH), via the incorporation of selected thioether succinimide cross-links between the PEG polymers and liposome nanoparticles. Dynamic light scattering (DLS) characterization confirmed that intact liposomes were released upon network degradation. Owing to the hierarchical structure of the network, multiple cargo molecules relevant for chemotherapies, namely doxorubicin (DOX) and cytochrome c, were encapsulated and simultaneously released from the hybrid hydrogels, with differential release profiles that were driven by degradation-mediated release and Fickian diffusion, respectively. This work introduces a facile approach for the development of advanced, hybrid drug delivery vehicles that exhibit novel chemical degradation.

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“…Currently, HA derivatives [173,182,214,215] and HAbased wound dressings, films or hydrogels enriched with other therapeutic agents [216,217] are being evaluated in order to understand if the cicatrisation process could be further enhanced. The wound healing properties of HA and its derivatives are being explored not only in dermatology, but also in other medical fields such as ophthalmology [56,218], otolaryngology [142], rhinology [219] and odontology [220].…”

Section: Wound Treatmentmentioning

confidence: 99%

Hyaluronic Acid in the 3<sup>rd</sup> Millennium

Fallacara¹,

Baldini²,

Manfredini³

et al. 2018

Preprint

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Since its first isolation in 1934, hyaluronic acid (HA) has been studied across a variety of research areas. This unbranched glycosaminoglycan consisting of repeating disaccharide units of N-acetyl-D-glucosamine and D-glucuronic acid is almost ubiquitary in humans and in other vertebrates. HA is involved in many key processes -including cell signaling, wound reparation, tissue regeneration, morphogenesis, matrix organization and pathobiology-, and has unique physico-chemical properties -such as biocompatibility, biodegradability, mucoadhesivity, hygroscopicity and viscoelasticity. For these reasons, exogenous HA has been investigated as drug delivery system and treatment in cancer, ophthalmology, arthrology, pneumology, rhinology, urology, aesthetic medicine and cosmetic. To improve and customize its properties and applications, HA can be subjected to chemical modifications: conjugation and crosslinking. The present review gives an overview regarding HA, describing its history, physico-chemical, structural and hydrodynamic properties, and biology -occurrence, biosynthesis (by hyaluronan synthases), degradation (by hyaluronidases and oxidative stress), roles, mechanisms of action and receptors. Furthermore, both conventional and recently emerging methods developed for the industrial production of HA and its chemical derivatization are presented. Finally, the medical, pharmaceutical and cosmetic applications of HA and its derivatives are reviewed, reporting examples of HA-based products which currently are on the market or are undergoing further investigations.

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Ophthalmic Uses of a Thiol-Modified Hyaluronan-Based Hydrogel

Cited by 38 publications

References 43 publications

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Liposome-Cross-Linked Hybrid Hydrogels for Glutathione-Triggered Delivery of Multiple Cargo Molecules

Hyaluronic Acid in the 3<sup>rd</sup> Millennium

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