Microtiter Tests for Detecting Antibody in Bovine Serum to Parainfluenza 3 Virus, Infectious Bovine Rhinotracheitis Virus, and Bovine Virus Diarrhea Virus

Rossi, C. R.; Kiesel, G. K.

doi:10.1128/aem.22.1.32-36.1971

Cited by 52 publications

(11 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All serum samples were tested using serum neutralization test for detection of specific neutralizing antibodies against all vaccinal viruses of Pneumo-4 commercial and prepared oil adjuvants. SNT applied in micro-titration plate technique following the procedure described by Rossi and Kiessel, (1971). The Serum Neutralizing Antibody titers of the tested serum samples were expressed as log10 of the reciprocal serum dilution that protects >50% of micro titration plate dilution wells, and these following the calculation formula of Reed and Muench, (1938).…”

Section: Serum Neutralization Test (Snt)mentioning

confidence: 99%

Improving performance of Pneumo-4 vaccine using montanide oil

El-Bagoury¹,

El-Habbaa²,

El-Sayed³

et al. 2017

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

Selecting of the adjuvant is one of the keys for the success of the vaccine application. Oil adjuvants, water-in-oil-in-water (W/O/W), have proved their efficacy in bovine vaccine as they induce rapid and long term immune response with various antigens. The present study was aimed towards improving and upgrading the locally prepared inactivated combined vaccine (Pneumo-4) containing Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhoea (BVD), Parainfleunza type 3 (PI-3) and Bovine Respiratory syncytial (BRS) viruses using Montanide oil ISA206 as adjuvant and compare its efficacy with the commercial gel adjuvanted one. Both the commercial and prepared oil Pneumo-4 vaccines were administered twice to 6-9 months old seronegative calves through intramuscular (I/M) route with dose 5 ml, 21 days apart. Humoral immune response was followed up in the sera of vaccinated calves using serum neutralization test (SNT). The result revealed that both adjuvanted vaccines gave a significant effect on all vaccinated animals but the oil adjuvanted vaccine was particularly better and effective than gel adjuvanted where oil adjuvanted vaccine induced early intense immune response and showed prolongation of antibody secretion in comparison to the Aluminium Hydroxide gel.

show abstract

Section: Serum Neutralization Test (Snt)mentioning

confidence: 99%

Improving performance of Pneumo-4 vaccine using montanide oil

El-Bagoury¹,

El-Habbaa²,

El-Sayed³

et al. 2017

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

show abstract

“…Antibodies to B. abortus were identified by plate agglutination (Alton et al, 1988). Sera were heat-inactivated at 56°C for 60 min and 2-fold dilutions were tested for end-point neutralizing antibodies to National Animal Disease Laboratory (NADL; USA) reference isolate of BVDV and LA (Los Angeles) reference strain of BHV-1 using a standard microtitration procedure (Rossi and Kiesel, 1971).…”

Section: Serological Testsmentioning

confidence: 99%

Reproductive Losses due toNeospora caninumin a Beef Herd in Argentina

Moore

Campero

Odeón

et al. 2003

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

Reproductive losses in a beef herd of 857 heifers with a pregnancy rate of 86.3% are described. After pregnancy testing, 69 abortions were seen during a 3 month period. Before calving season, three heifers had delivered pre-mature non-viable calves. Serum samples from 58 of 69 aborted heifers were available for serological tests. In order to compare the seroprevalence in non-aborted vs. aborted heifers, 214 pregnant animals were bleed during the abortion storm. In addition, blood samples were collected from two heifers with pre-mature calves and from 16 heifers with their calves prior to colostrum intake. All available serum samples were tested for Neospora caninum antibodies using an indirect fluorescence antibody test (IFAT). Fifty-nine of 290 (20.3%) evaluated heifers were seropositive. Heifers that aborted and heifers with pre-mature calves were more likely to be seropositive than pregnant heifers and heifers with normal calves [odds ratio (OR), 12.01; 95% CI, 6.18-23.30]. Vaginal mucus from four aborted heifers, and samples from two aborted foetuses and three pre-mature calves were available. Laboratory tests for Tritrichomonas foetus, bacterial and viral isolation, and histological examination were performed. Culture from vaginal mucus and foetal samples were negative. Histological lesions consistent with neosporosis and positive immunohistochemistry (IHC) to N. caninum were found in one aborted foetus and in one pre-mature calf. It is the first description of reproductive losses because of N. caninum in beef herds in Argentina.

show abstract

“…The serum neutralization tests for BHV-1 and BVDV were performed following the already described methods (ROSSI and KIESEL, 1971). The viral strains were: LA of BHV-1, and NADL of BVDV.…”

Section: Laboratory Testingmentioning

confidence: 99%

Serological Survey of Viral Antibodies in Llamas (Lama glama) in Argentina

Puntel

Fondevila

Viera

et al. 1999

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

This study analysed sera from 390 llamas (Lama glama) from nine farms located in three different Argentine provinces: Buenos Aires, Cordoba and Jujuy. The samples were tested for antibodies against 8 virus known to infect cattle: bovine herpesvirus type 1 (BHV-1), bovine viral diarrhea virus (BVDV), bovine adenovirus (BAdV III), bovine enterovirus (BEV), bovine rotavirus (BRV), bluetongue virus (BTV), bovine leukaemia virus (BLV), and foot-and-mouth virus (FMDV) by conventional methods such as seroneutralization, immunoperoxidase staining, and agar gel immunodiffusion. The antibody prevalences detected in llamas were: BHV-1 in 0.77% (3/390), BVDV in 2.05% (8/390), BAdV III in 5.13% (20/390), BEV in 4.10% (16/390), BRV in 87.69% (342/390). No antibodies against BTV, BLV and VIAA (FMDV infection associated antigen) were detected.

show abstract

Microtiter Tests for Detecting Antibody in Bovine Serum to Parainfluenza 3 Virus, Infectious Bovine Rhinotracheitis Virus, and Bovine Virus Diarrhea Virus

Cited by 52 publications

References 3 publications

Improving performance of Pneumo-4 vaccine using montanide oil

Improving performance of Pneumo-4 vaccine using montanide oil

Reproductive Losses due toNeospora caninumin a Beef Herd in Argentina

Serological Survey of Viral Antibodies in Llamas (Lama glama) in Argentina

Contact Info

Product

Resources

About