Microspore culture of Hordeum vulgare L.: the influence of density and osmolality

Hoekstra, S.; Zijderveld, M.H. van; Heidekamp, F.; Mark, F. van der

doi:10.1007/bf00233415

Cited by 103 publications

(45 citation statements)

References 18 publications

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“…The regenerated plants were completely fertile doubled haploids (Hoekstra et al, 1992;1993;Hu and Kasha, 1999). The high frequency of completely fertile plants indicates that chromosome doubling must occur very early, most likely at the time of induction (Kasha et al, 2001;Seguí-Simarro and Nuez, 2008).…”

Section: Discussionmentioning

confidence: 99%

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

Kahrizi

Mohammadi²

2009

Acta Agronomica Hungarica

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This research aimed to study the androgenesis and spontaneous chromosome doubling of five barley genotypes using an isolated in vitro microspore culture technique, involving a completely randomized design (CRD) with three replications. Statistical analysis of embryogenesis and cytogenetic results showed that genotype had a significant effect on haploid embryogenesis (P<0.01) and on spontaneous chromosome doubling (P<0.05). The genotype Igri was found to have the highest potential to produce haploid embryos (1577 embryos from 100 anthers), followed by the genotypes Boyer/Rojo, Afzal/Turkman/Kavir, Ashar/Hebo and Agrigashar/Matico with 369, 304, 278 and 150 embryos from 100 anthers, respectively. The highest percentage of spontaneous chromosome doubling (76%) was observed for the genotype which had the lowest embryogenesis (Agrigashar/Matico) and the lowest (65%) for the genotype with the highest androgenic capacity (Igri). Microspore embryogenesis also showed comparatively higher genotypic (109.2) and phenotypic (109.5) coefficients of variation, heritability (99.62) and genetic advance (1206.77), indicating the pre-dominance of additive gene action in the control of this character in the material studied. Estimates of genetic parameters (PCV, GCV and heritability) for microspore embryogenesis were higher than for spontaneous doubled haploids. These results indicated that selection for androgenic capacity would be more effective than for spontaneous doubled haploids. The findings showed a negative relationship (r= -0.68) between embryogenesis and spontaneous chromosome doubling in the barley genotypes studied. All the large embryos used had high regenerability and good plantlet formation.

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Section: Discussionmentioning

confidence: 99%

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

Kahrizi

Mohammadi²

2009

Acta Agronomica Hungarica

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“…The mean viability of rye microspores after isolation from the analyzed populations from Danko was 87.6%, while for microspores from rye populations obtained from Poznań was 87.2%. Hoekstra et al (1993) characterized different types of barley microspores on the first days of culture. They showed that only microspores with a markedly enlarged diameter divide after a 3-day incubation in a medium that induces androgenesis.…”

Section: Resultsmentioning

confidence: 99%

The effect of cold temperature stress on the viability of rye ( Secale cereale L.) microspores

Mikołajczyk

Broda²,

Weigt³

2012

bta

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Rye has been generally considered to be recalcitrant to the androgenesis induction in vitro. The haploids of Secale cereale are not easily obtainable and the isolated microspore technique application is difficult, thus is performed occasionally, and in a limited number of strains and cultivars. The aim of the conducted experiment was to assess the frequency of mechanical damage in microspores after their isolation in the mortar, and the effect of the pretreatment of spikes at 4EC on microspore viability and the course of the culture. The analyses in the isolated microspore culture were conducted on 30 genotypes of rye from two breeding programs and differing in their origin. Spikes with microspores in the unicellular stage were cut and exposed to a temperature of 4EC for a period of 2 to 42 days. Microspore observations were conducted immediately after the microspore isolation in order to assess the viability and percentage of the damaged ones. In order to study the mitotic divisions first, cultures of rye microspores were examined after 3 days. The highest microspore viability after isolation was observed for strain 1283C -91.9% and inbred line S1152/10 -91.8%, while it was the lowest for F 1 hybrid S02779/10 -79.8% and strain 1252E -84%, respectively. It was found that to maintain high (microspore -to mozna opuscic) viability, microspores must be isolated after low temperature stress (+4EC) applied for up to 7 days of culture. A considerable extension of the duration of low temperature (36-42 days) resulted in a reduction of the microspore isolation efficiency in rye (a lower number of microspores in the obtained suspension) and a slight reduction of microspore viability.

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“…It is possible to observe that while part of the microspores have adopted the sporophytic route the majority of them have degenerated. According to Hoekstra et al (1993), only 25% of the viable, potentially embryogenic microspores effectively divide themselves when cultured in vitro. Not all of these proceed their sporophytic development.…”

Section: Resultsmentioning

confidence: 99%

Ontogenetic events in androgenesis of Brazilian barley genotypes

2000

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This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite and nutrient solution.Key words: anther culture, doubled haploids. RESUMO Método para obtenção de duplo-haplóides de genótipos brasileiros de cevadaAnteras de cevada (Hordeum vulgare L.) foram cultivadas in vitro, fazendo com que houvesse uma reversão no desenvolvimento dos micrósporos para um modo esporofítico de desenvolvimento. Uma primeira mitose simétrica, seguida de uma seqüência de mitoses, levou à formação de micrósporos multinucleados. A regeneração de plantas ocorreu tanto por embriogênese direta quanto pela formação de calos, com posterior diferenciação via embriogênese indireta ou organogênese. Os embriões e calos androgenéticos mostraram grande potencial para regeneração, e plantas verdes e albinas foram regeneradas no mesmo meio utilizado para indução, sendo a transferência para outro meio necessária somente para dar prosseguimento ao desenvolvimento e ao enraizamento das plântulas. A possibilidade de induzir pró-embrióides e regenerar plantas em um único passo, sem necessidade de transferência para um meio específico de regeneração, aumenta a rapidez e a eficiência da técnica de cultura de anteras.Palavras-chave: cultura de anteras, duplo-haplóides.

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Microspore culture of Hordeum vulgare L.: the influence of density and osmolality

Cited by 103 publications

References 18 publications

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

The effect of cold temperature stress on the viability of rye ( Secale cereale L.) microspores

Ontogenetic events in androgenesis of Brazilian barley genotypes

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