Microshoots encapsulation and plant conversion of Musa sp. cv. 'Grand Naine'

Abstract

Synthetic seed technologies are useful tools for the field delivery of in vitro derived plantlets. In the present study, different encapsulation procedures and their efficacy in the plantlet regeneration using microshoots of banana cv. 'Grand Naine' were evaluated. Two encapsulation systems were evaluated: i) single encapsulation in beads or droplet hardening method; and ii) double layer or hollow beads. The use of different compounds to enhance the capsule conservation and the conversion to plantlets was also evaluated. The conversion capacity was assessed in vitro on water-agar culture medium or in ex vitro conditions with Gerbox® boxes. The single encapsulation system showed 80% conversion. The capsules with MS saline formulation treated with 100mM KNO3 showed 76% conversion. Capsules with 1.5g L-1 activated charcoal, and 0.5g L-1 benomyl sucrose-free capsules showed 75% conversion. The encapsulated and non-encapsulated microshoots exhibited 100% germination in response to MS culture medium, and polyethylene glycol after 10 days of storage at 4°C. Sucrose-free capsules showed significantly higher germination (83.3%) than those sucrose-enriched capsules (56.7%). The ex vitro conversion of encapsulated microshoots was 20% in the Gerbox™. These results indicate the feasibility using synthetic seeds in the large-scale micropropagation of banana cv. 'Grand Naine'.

Influência da composição da matriz de encapsulamento de microbrotos de banana (Musa sp.) cv. Prata-Anã clone Gorutuba

“…pode diminuir os custos de produção pela eliminação das etapas de enraizamento e aclimatação; além da possibilidade do estabelecimento dos propágulos diretamente a campo num estágio mais precoce do que em sistemas convencionais de propagação (SAIPRASAD, 2001). Essa técnica foi utilizada com sucesso por Sandoval-Yugar et al (2009), na cultivar 'Grande Naine', e Hassanein et al (2011), na cultivar Hindi, obtendo altas taxas de conversão, 75% e 98.34%, respectivamente. Apesar dos resultados obtidos com esta tecnologia, há necessidade de inovações e melhorias quanto à adequação dos tipos de concentrações dos constituintes a serem introduzidos à matriz de encapsulamento.…”

Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

“…If required, the encapsulated propagules or commonly called "synthetic seeds," could possibly be used to facilitate short-term storage required for germplasm exchange or delayed sowing [5,6]. In most studies on synthetic seeds, a plant propagule is often enclosed within a single alginate coating [7,8], while variations of this approach, including double coating layers, have also been investigated [9][10][11][12].…”

Cryopreservation and effect of lighting conditions and cytokinins on in vitro multiplication of Miconia ligustroides (DC.) Naudin

“…Through the correct use of these techniques, regeneration of the meristematic regions (Ferreira et al, 2007) can be established to encapsulate the lateral buds of the material regenerated in an alginate matrix (Sandoval-Yugar et al, 2009). When combined with the use of growth regulators (Jona and Webb, 1978;Bhojwani et al, 1984) and adequate light sources (Rocha et al, 2010(Rocha et al, , 2013 this technique can generate multiple healthy shoots in a short period from a single plant germinated in vitro.…”