Microscale creep and stress relaxation experiments with individual collagen fibrils

Yang, Fan; Das, Debashish; Chasiotis, Ioannis

doi:10.1016/j.optlaseng.2021.106869

Cited by 11 publications

(3 citation statements)

References 58 publications

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“…This measurement was possible due to the bidirectional closed loop of the MEMS probe, which allowed to compensate micromotion movements and adapt to the dynamic environment. Previously the use of MEMS-based force sensors complemented with atomic force microscopy (AFM)-based nanoindentation allowed to measure nanoscale properties from individual collagen fibers [41][42][43][44] with high resolution in the range of nN, however such technology has been suitable for in-vitro studies, and it is well known that explanted tissue modifies its mechanical properties. To our knowledge, nerves and other neuronal structures mechanical properties have been defined from explanted tissue and no detailed information is available to take in account the epineurium breakage and fibers composition dependence 8,10,40,45 .…”

Section: Discussionmentioning

confidence: 99%

Biomechanics Characterization of Autonomic and Somatic Nerves by High Dynamic Closed-Loop MEMS force sensing

González-González

Alemansour

Maroufi

et al. 2023

Preprint

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The biomechanics of peripheral nerves are determined by the blood-nerve barrier (BNB), together with the epineural barrier, extracellular matrix, and axonal composition, which maintain structural and functional stability. These elements are often ignored in the fabrication of penetrating devices, and the implant process is traumatic due to the mechanical distress, compromising the function of neuroprosthesis for sensory-motor restoration in amputees. Miniaturization of penetrating interfaces offers the unique opportunity of decoding individual nerve fibers associated to specific functions, however, a main issue for their implant is the lack of high-precision standardization of insertion forces. Current automatized electromechanical force sensors are available; however, their sensitivity and range amplitude are limited (i.e. mN), and have been tested only in-vitro. We previously developed a high-precision bi-directional micro-electromechanical force sensor, with a closed-loop mechanism (MEMS-CLFS), that while measuring with high-precision (−211.7μN to 211.5μN with a resolution of 4.74nN), can be used in alive animal. Our technology has an on-chip electrothermal displacement sensor with a shuttle beam displacement amplification mechanism, for large range and high-frequency resolution (dynamic range of 92.9 dB), which eliminates the adverse effect of flexural nonlinearity measurements, observed with other systems, and reduces the mechanical impact on delicate biological tissue. In this work, we use the MEMS-CLFS for in-vivo bidirectional measurement of biomechanics in somatic and autonomic nerves. Furthermore we define the mechanical implications of irrigation and collagen VI in the BNB, which is different for both autonomic and somatic nerves (~ 8.5-8.6 fold density of collagen VI and vasculature CD31+ in the VN vs ScN). This study allowed us to create a mathematical approach to predict insertion forces. Our data highlights the necessity of nerve-customization forces to prevent injury when implanting interfaces, and describes a high precision MEMS technology and mathematical model for their measurements.

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Section: Discussionmentioning

confidence: 99%

Biomechanics Characterization of Autonomic and Somatic Nerves by High Dynamic Closed-Loop MEMS force sensing

González-González

Alemansour

Maroufi

et al. 2023

Preprint

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“…The spatial hierarchy, which spans the range of scales from nanometers to millimeters, is responsible for many of collagen’s mechanical properties, including large extensibility and strain hardening . Collagen hydration is crucial to this structure–function relationship: for example, while interstitial water plays a well-understood role in viscoelasticity of bulk tissues, intrafibrillar water is a key determinant of the viscoelastic behavior of individual collagen fibrils . Collagen hydration modulates both the elastic and the viscous component of tissue mechanical response, thus contributing to the difference in stress–strain behaviors at static, slow-changing, and rapidly changing loads. , In combination with fiber mineralization, this serves as the physical basis for the mechanical strength of bone.…”

Section: Tissue Mechanics Tissue Engineering and Biomaterialsmentioning

confidence: 99%

Hydrated Collagen: Where Physical Chemistry, Medical Imaging, and Bioengineering Meet

Momot

2022

J. Phys. Chem. B

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“…As isolated collagen fibers are nearly three orders of magnitude stiffer than elastic fibers [30], it is normally considered that elastin fibers play a leading role in withstanding deformation of skin at lower stress, while collagen fibers become the major load bearing component at higher stress [31]. To learn more about the individual tissue components, several studies have focused on the mechanical behavior of isolated collagen fibrils [32][33][34], which show a nonlinear behavior when deformed, with a characteristic "J"-shape stress-strain curve that can be well fitted by a second-order polynomial [32]. The strain within isolated collagen fibrils was found to be considerably smaller than in the whole tendon.…”

mentioning

confidence: 99%

Three-dimensional Characterization of Mechanical Properties and Microstructures of Human Dermal Skin

Zhou,

González,

Haasterecht

et al. 2023

Preprint

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The intact and healthy skin forms a barrier to the outside world and protects the body from mechanicalimpact. The skin is a complex structure with unique mechano-elastic properties. To better direct the design of biomimetic materials and induce skin regeneration in wounds with optimal outcome, more insight is required in how the mechano-elastic properties emerge from skin’s main constituents, collagen and elastin fibers. Here, we employed two-photon auto excited fluorescence (2PEF) and secondharmonic generation (SHG) microscopy to characterize collagen and elastin fibers in 3D in 24 humandermis skin samples. We performed uniaxial stretching experiments to determine mechanical tissueparameters from the resulting strain-stress curves. We also monitored changes in collagen and elastin alignment during uniaxial stretching in real-time. The strain-stress curves show a large variation, with an average Young’s modules in the heel and linear elastic regions of 0.1 MPa and 21 MPa. We performed a comprehensive analysis of the correlation between key mechanical properties, Young’smodulus, maximal strain and maximal stress to micro-structural parameters, fiber density, diameterand orientation, and age. To capture non-linear dependencies between the four characteristics and the micro-structural properties, we calculated the so-called distance correlation (DC) between the variousvariables. Age was found to correlate negatively with Youngs modulus and collagen density. Elastin fibers aligned significantly in both the heel and linear regions, the collagen bundles engaged andoriented mainly in the linear region. This research advances our understanding of skin biomechanicsand yields input for future first principles full modeling of skin tissue.

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Microscale creep and stress relaxation experiments with individual collagen fibrils

Cited by 11 publications

References 58 publications

Biomechanics Characterization of Autonomic and Somatic Nerves by High Dynamic Closed-Loop MEMS force sensing

Biomechanics Characterization of Autonomic and Somatic Nerves by High Dynamic Closed-Loop MEMS force sensing

Hydrated Collagen: Where Physical Chemistry, Medical Imaging, and Bioengineering Meet

Three-dimensional Characterization of Mechanical Properties and Microstructures of Human Dermal Skin

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