MicroRNA expression in chronic lymphocytic leukemia developing autoimmune hemolytic anemia

Ferrer, Gerardo; Navarro, Alfons; Hodgson, Kate; Aymerich, Marta; Pereira, Arturo; Baumann, Tycho; Monzó, Mariano; Moreno, Carol; Montserrat, Emili

doi:10.3109/10428194.2012.763123

Cited by 24 publications

(21 citation statements)

References 58 publications

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“…miR-660 is a tumor-related miRNA and extensively involved in many biological processes. Indeed, this is consistent with our tissue expression profile showing that miR-660 was ubiquitously expressed in different cattle tissues [Ferrer et al, 2013]. Recent reports gave a role for miR-660 as a lung tumor suppressor miRNA [Fortunato et al, 2014], which may be related to significantly high expression in adult lung during our research.…”

Section: Discussionsupporting

confidence: 92%

Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation

2017

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MicroRNA are a series of small non-coding RNAs that have emerged as critical regulators of skeletal muscle development. Here, we concentrated on the function of miR-660 during bovine skeletal myogenesis from our previous high-throughput sequencing results, then analyzed its expression profiles and characterized related functional roles. Overexpression of miR-660 significantly attenuated myogenic differentiation of C2C12 cells, whereas miR-660 inhibition enhanced C2C12 differentiation. Dual-Luciferase Reporter Assay went for demonstrating that miR-660 directly targeted the 3'-UTR of Rho guanine nucleotide exchange factor 12 (ARHGEF-12). Furthermore, we found an inverse relationship between the expression of miR-660 and ARHGEF12 in both gain- and loss-of-function studies: overexpression of miR-660 declined the mRNA and protein expressions of ARHGEF12 in C2C12 cells differentiation; however, knockdown of miR-660 had completely opposite results. Taken together, these results offered a new perspective for miR-660 in skeletal muscle differentiation. J. Cell. Biochem. 118: 2387-2394, 2017. © 2017 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 92%

Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation

2017

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“…Over-expression of miR181a and miR16 in MSG tissues of SS patients has been associated with decreased salivary function [9]. Furthermore, elevated expression of miR16 has been associated with human Graves' disease, rheumatoid arthritis (RA) and primary biliary cirrhosis [19][20][21], deregulated expression of miR181a with systemic lupus erythematosus (SLE) [22,23], miR223 with RA, SLE, dermatomyositis and autoimmune haemolytic anaemia [24][25][26] and miR483-5p with paediatric immune thrombocytopenic purpura [27].…”

Section: Discussionmentioning

confidence: 99%

Study of microRNAs (miRNAs) that are predicted to target the autoantigens Ro/SSA and La/SSB in primary Sjögren's Syndrome

Gourzi

Kapsogeorgou

Kyriakidis

et al. 2015

Clinical and Experimental Immunology

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1 These authors contributed equally to the study. SummaryThe elevated tissue expression of Ro/SSA and La/SSB autoantigens appears to be crucial for the generation and perpetuation of autoimmune humoral responses against these autoantigens in Sj€ ogren's syndrome (SS). The mechanisms that govern their expression are not known. miRNAs, the posttranscriptional regulators of gene expression, might be implicated. We have identified previously the miRNAs let7b, miR16, miR181a, miR200b-3p, miR200b-5p, miR223 and miR483-5p that are predicted to target Ro/SSA [Ro52/tripartite motif-containing protein 21 (TRIM21), Ro60/TROVE domain family, member 2 (TROVE2)] and La/SSB mRNAs. To study possible associations with autoantigen mRNA expression and disease features, their expression was investigated in minor salivary gland (MSG) tissues, peripheral blood mononuclear cells (PBMC) and long-term cultured non-neoplastic salivary gland epithelial cells (SGEC) from 29 SS patients (20 of 29 positive for autoantibodies to Ro/SSA and La/SSB) and 24 sicca-complaining controls. The levels of miR16 were up-regulated in MSGs, miR200b-3p in SGECs and miR223 and miR483-5p in PBMCs of SS patients compared to siccacomplaining controls. The MSG levels of let7b, miR16, miR181a, miR223 and miR483-5p were correlated positively with Ro52/TRIM21-mRNA. miR181a and miR200b-3p were correlated negatively with Ro52/TRIM21 and Ro60/ TROVE2 mRNAs in SGECs, respectively, whereas let7b, miR200b-5p and miR223 associated with La/SSB-mRNA. In PBMCs, let7b, miR16, miR181a and miR483-5p were correlated with Ro52/TRIM21, whereas let7b, miR16 and miR181a were also associated with La/SSB-mRNA expression. Significantly lower miR200b-5p levels were expressed in SS patients with mucosa-associated lymphoid tissue (MALT) lymphoma compared to those without. Our findings indicate that miR16, miR200b-3p, miR223 and miR483-5p are deregulated in SS, but the exact role of this deregulation in disease pathogenesis and autoantigen expression needs to be elucidated.

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“…as lung cancer (18), breast cancer (19), multiple myeloma (20) and chronic lymphocytic leukemia (21). However, the clinical significance and function of miR-660-5p in RCC remained to be explored.…”

Section: Mir-660-5p Is Associated With Cell Migration Invasion Prolmentioning

confidence: 99%

miR‑660‑5p is associated with cell migration, invasion, proliferation and apoptosis in renal cell carcinoma

Chen

Jin

et al. 2017

Mol Med Report

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Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system with poor prognosis. microRNAs (miRNAs) are a class of small, non‑coding RNA molecules that serve important roles in biological and pathological processes in several types of human tumors. miRNA (miR)‑660‑5p is dysregulated in many human cancers; however, its role in renal cell carcinoma is currently unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction was performed to examine the expression levels of miR‑660‑5p in RCC tissues and paired normal adjacent tissues (NATs). To determine the function of miR‑660‑5p in RCC cells, wound‑healing and Matrigel assays were performed to determine the effects of miR‑660‑5p on cell migration and invasion, respectively. MTT and Cell Counting kit‑8 assays were performed to determine the effects of miR‑660‑5p on RCC cell proliferation. In addition, flow cytometric analysis was performed to validate the effects of miR‑660‑5p on apoptosis. The results indicated that miR‑660‑5p expression was downregulated in RCC tissues compared with NATs. Restoration of miR‑660‑5p expression using synthetic mimics may suppress cell migration, invasion and proliferation, and induce cell apoptosis, while using synthetic inhibitors may promote cell migration, invasion and proliferation, and suppress cell apoptosis. These results suggested that miR‑660‑5p may serve a tumor suppressive role in RCC tumorigenesis.

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MicroRNA expression in chronic lymphocytic leukemia developing autoimmune hemolytic anemia

Cited by 24 publications

References 58 publications

Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation

Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation

Study of microRNAs (miRNAs) that are predicted to target the autoantigens Ro/SSA and La/SSB in primary Sjögren's Syndrome

miR‑660‑5p is associated with cell migration, invasion, proliferation and apoptosis in renal cell carcinoma

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