miR‑660‑5p is associated with cell migration, invasion, proliferation and apoptosis in renal cell carcinoma

He, Tao; Chen, Peijie; Jin, Lü; Hu, Jia; Li, Yifan; Zhou, Liang; Yang, Shangqi; Mao, Xiangming; Gui, Yaoting; Chen, Yun; Lai, Yongqing

doi:10.3892/mmr.2017.8052

Cited by 11 publications

(9 citation statements)

References 39 publications

(47 reference statements)

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“…miR-29a-3p has been related to many different types of cancer, like gastric cancer [41] and hepatocellular carcinoma [42]. miR-660-5p seems to regulate the proliferation, migration, and invasion of human breast cancer cells [43] and it has been related to cell migration, invasion, proliferation and apoptosis in renal cell carcinoma [44]. miR-331-3p has been recently postulated as predictor of recurrence in esophageal adenocarcinoma [45] and as regulator in pancreatic cancer [46].…”

Section: Discussionmentioning

confidence: 99%

microRNAs and Markers of Neutrophil Activation as Predictors of Early Incidental Post-Surgical Pulmonary Embolism in Patients with Intracranial Tumors

Oto

Plana

Solmoirago

et al. 2020

Cancers

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Venous thromboembolism (VTE) is a common complication of cancer that severely increases morbidity and mortality. Patients with intracranial tumors are more likely to develop VTE than patients with cancers at other sites. Conversely, limited tools exist to identify patients with high thrombotic risk. Upon activation, neutrophils release their content through different mechanisms triggering thrombosis. We explored the ability of microRNAs (miRNAs) and plasma markers of neutrophil activation measured before surgery to predict the risk of early post-surgical pulmonary embolism (PE) in glioma and meningioma patients. We recruited and prospectively followed 50 patients with glioma and 50 with meningioma, 34% of whom in each group developed an early objectively-diagnosed post-surgical PE. We measured miRNA expression and neutrophil markers (cell-free DNA, nucleosomes, calprotectin and myeloperoxidase) before surgery. In glioma patients, we adjusted and validated a predictive model for post-surgical PE with 6 miRNAs: miR-363-3p, miR-93-3p, miR-22-5p, miR-451a, miR-222-3p and miR-140-3p (AUC = 0.78; 95% Confidence Interval (CI) [0.63, 0.94]) and another with cfDNA and myeloperoxidase as predictors (AUC = 0.71; 95% CI [0.52, 0.90]). Furthermore, we combined both types of markers and obtained a model with myeloperoxidase and miR-140-3p as predictors (AUC = 0.79; 95% CI [0.64, 0.94]). In meningioma patients we fitted and validated a predictive model with 6 miRNAs: miR-29a-3p, miR-660-5p, miR-331-3p, miR-126-5p, miR-23a-3p and miR-23b-3p (AUC = 0.69; 95% CI [0.52, 0.87]). All our models outperformed the Khorana score. This is the first study that analyzes the capability of plasma miRNAs and neutrophil activation markers to predict early post-surgical PE in glioma and meningioma patients. The estimation of the thrombotic risk before surgery may promote a tailored thromboprophylaxis in a selected group of high-risk patients, in order to minimize the incidence of PE and avoid bleedings.

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Section: Discussionmentioning

confidence: 99%

microRNAs and Markers of Neutrophil Activation as Predictors of Early Incidental Post-Surgical Pulmonary Embolism in Patients with Intracranial Tumors

Oto

Plana

Solmoirago

et al. 2020

Cancers

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“…In renal cell carcinoma, miR-660 was down-regulated and could suppress cell migration, invasion and proliferation, and induce cell apoptosis. 14…”

Section: Discussionmentioning

confidence: 99%

circFBXL5 promotes breast cancer progression by sponging miR‐660

Zhou

Tang

Zhao

et al. 2019

J Cellular Molecular Medi

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Increasing studies have revealed that circular RNAs (circRNAs) play important roles in cancer progression. However, the potential involvement of circRNAs in breast cancer metastasis to lung is not clear so far. In this study, we conducted circular RNA microarrays of primary breast cancer tissues and lung metastatic tissues. The results revealed that circFBXL5 (hsa_circ_0125597) up‐regulated the most in lung metastatic tissues. Survival analysis revealed that high levels of circFBXL5 correlated with worse outcome of breast cancer. Further experiments showed that knockdown of circFBXL5 inhibited breast cancer cell proliferation and migration to lung. Mechanism study showed that circFBXL5 acted as a sponge for miR‐660 and compete binding to miR‐660 with SRSF6, leading to increased expression of SRSF6. Collectively, our study highlighted the regulatory function of the circFBXL5/miR‐660/SRSF6 pathway in breast cancer progression, which could be potential therapeutic targets for breast cancer.

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“…Studies have shown that miR‐660‐5p regulates the malignancy of breast cancer cells by suppressing the expression of TFCP2, and is a novel therapeutic target for clinical treatment and a potential prognostic indicator . Moreover, miR‐660‐5p acts as a tumor suppressor in renal cell carcinoma and may regulate cell migration, proliferation, and apoptosis . However, the role of miR‐660‐5p in the pathogenesis of lung cancer remains unknown.…”

Section: Introductionmentioning

confidence: 99%

“…17,18 Moreover, miR-660-5p acts as a tumor suppressor in renal cell carcinoma and may regulate cell migration, proliferation, and apoptosis. 19 However, the role of miR-660-5p in the pathogenesis of lung cancer remains unknown. This study aimed to elucidate the role of miR-660-5p in organ-specific metastasis of lung cancer cells and the molecular mechanism underlying its functions.…”

Section: Introductionmentioning

confidence: 99%

“…17 Another study reported that miR-660-5p expression was significantly downregulated in renal cell carcinoma and could act as a tumor suppressor to regulate cell migration, proliferation, and apoptosis. 19 Also, dysregulated miR-660-5p expression has been reported in Hodgkin's lymphoma 45 and multiple myeloma. 46 In the present study, miR-660-5p was significantly upregulated in all four organ-specific metastasis cell lines with lower nm23-H1 expression than normal L9981-Luc.…”

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confidence: 99%

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Nm23‐H1 inhibits lung cancer bone‐specific metastasis by upregulating miR‐660‐5p targeted SMARCA5

Deng

et al. 2020

Thoracic Cancer

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Background Nm23‐H1 gene has been found to be an inhibitor of tumor metastasis in lung cancer. MicroRNAs (miRNAs) play key roles in tumor metastasis through multiple signaling pathways. This study explored whether the nm23‐H1 gene could inhibit invasion and metastasis of lung cancer cells by regulating miRNA‐660‐5p targets. Methods Quantitative real‐time PCR (qRT‐PCR) and western blots were used to measure the expression of nm23‐H1 and miR‐660‐5p of various human lung cancer cell lines. Cell counting kit‐8 (CCK‐8), wound‐healing and transwell assay were carried out to assess cell proliferation, migration and invasion of each cell line. Xenograft were applied to determine in vivo effects of miR‐660‐5p among nude mice. Luciferase assay and western blot were performed to determine the target gene of miR‐660‐5p. Results We found that high expression of nm23‐H1 correlated with decreased miRNA‐660‐5p expression. Inhibiting miR‐660‐5p suppressed lung cancer cells progression significantly in vitro, whereas overexpression of miR‐660‐5p facilitated tumor growth and bone metastasis in vivo. In addition, as the potential target gene of miR‐660‐5p, SMARCA5 overexpression in vitro suppressed tumor progression and osteolytic metastasis associated RANKL signaling, which is congruent with the effect of nm23‐H1 on the lung cancer cells. Conclusion Nm23‐H1 inhibits tumor progression and bone‐specific metastasis of lung cancer by regulating miR‐660‐5p/SMARCA5/RANKL axis, which indicates the related genes may serve as potential targets for the treatment of human lung cancer. Key points Significant findings of the study High expression of nm23‐H1 correlated with decreased miRNA‐660‐5p expression. Further, downregulation of miR‐660‐5p significantly suppressed the tumor progression and bone‐specific metastasis of lung cancer cells. What this study adds This is the first study to show an inverse association between nm23‐H1 and miR‐660‐5p, and confirm that nm23‐H1 inhibits tumor progression and bone‐specific metastasis of lung cancer by regulating miR‐660‐5p/SMARCA5/RANKL axis.

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miR‑660‑5p is associated with cell migration, invasion, proliferation and apoptosis in renal cell carcinoma

Cited by 11 publications

References 39 publications

microRNAs and Markers of Neutrophil Activation as Predictors of Early Incidental Post-Surgical Pulmonary Embolism in Patients with Intracranial Tumors

microRNAs and Markers of Neutrophil Activation as Predictors of Early Incidental Post-Surgical Pulmonary Embolism in Patients with Intracranial Tumors

circFBXL5 promotes breast cancer progression by sponging miR‐660

Nm23‐H1 inhibits lung cancer bone‐specific metastasis by upregulating miR‐660‐5p targeted SMARCA5

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