circFBXL5 promotes breast cancer progression by sponging miR‐660

Zhou, Huamao; Tang, G.H.; Zhao, Ming; Xie, Liping; Xie, Yuanjie; Zhang, Zhiwei; He, Xiu‐Sheng

doi:10.1111/jcmm.14737

Cited by 20 publications

(17 citation statements)

References 18 publications

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“…For example, circVAPA regulates cell proliferation and migration by sponging miR‐130a‐5p in breast cancer 26 . CircFBXL5 contributes to the progression of breast cancer by interacting with miR‐660 27 . In the present study, miR‐509‐3p was identified by bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 74%

Circ‐PRMT5 promotes breast cancer by the miR‐509‐3p/TCF7L2 axis activating the PI3K/AKT pathway

Jia

Zhang

et al. 2020

The Journal of Gene Medicine

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BackgroundBreast cancer is the most prevalent malignancy occurring in females. In recent years, emerging evidence has suggested that circular RNAs are involved in the development of multiple cancers. Circ‐PRMT5 has recently attracted attention as a tumor‐promoting circular RNA. In the present study, we focused on exploring the biological effects of circ‐PRMT5 in breast cancer.MethodsA quantitative real‐time polymerase chain reaction was used to determine the expression of circ‐PRMT5 in breast cancer. In vitro experiments, including cell‐counting kit‐8, 5‐ethynyl‐2'‐deoxyuridine, flow cytometry and tube formation assays, were performed to test the effects of circ‐PRMT5 on the cellular progression of breast cancer. Bioinformatic analysis, luciferase reporter, radioimmunoprecipitation and RNA‐pull down assays were performed to predict the potential microRNAs interacting with circ‐PRMT5 and mRNAs that can be targeted by miR‐509‐3p.ResultsCirc‐PRMT5 is up‐regulated in breast cancer tissues and cells. Importantly, an elevation of circ‐PRMT5 indicates a poor prognosis in patients with breast cancer. Functionally, knockdown of circ‐PRMT5 suppresses cell proliferation and angiogenesis and increases cell apoptosis in breast cancer. Mechanistically, we identified that circ‐PRMT5 up‐regulates TCF7L2 expression by acting as a miR‐509‐3p sponge. The negative expression correlation between miR‐509‐3p and circ‐PRMT5 or TCF7L2 in clinical tissues was further demonstrated. Rescue assays showed that TCF7L2 overexpression reverses the antitumoral effects of circ‐PRMT5 knockdown on breast cancer cell processes. Additionally, we demonstrated that circ‐PRMT5 activates the phosphoinositide 3‐kinase (PI3K)/AKT pathway by up‐regulation of TCF7L2.ConclusionsOverall, our data indicate that the circ‐PRMT5/miR‐509‐3p/TCF7L2 axis can aggravate the malignant character of breast cancer cells by the regulation of the PI3K/AKT pathway.

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Section: Discussionmentioning

confidence: 74%

Circ‐PRMT5 promotes breast cancer by the miR‐509‐3p/TCF7L2 axis activating the PI3K/AKT pathway

Jia

Zhang

et al. 2020

The Journal of Gene Medicine

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“…Circular RNAs (circRNAs) are a class of RNAs that have been studied a lot in recent years and have received extensive attention due to their unique covalently closed structure [7]. Many studies have demonstrated that circRNAs can be involved in the proliferation and metastasis of cancer cells to play a pro-cancer or anticancer effect in cancer progressions, such as circFMN2, circFBXL5 and circGRAMD18 [8][9][10]. In OSCC, hsa_ circ_0004491 was found to be lowly expressed in OSCC, and its knockdown could enhance the metastasis ability of OSCC [11].…”

Section: Introductionmentioning

confidence: 99%

Circ_0000140 restrains the proliferation, metastasis and glycolysis metabolism of oral squamous cell carcinoma through upregulating CDC73 via sponging miR-182-5p

Guo

Zhang

2020

Cancer Cell Int

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Background: Oral squamous cell carcinoma (OSCC) is a more common cancer in the world. Emerging evidence suggests that circular RNAs (circRNAs) participate in the progression of OSCC. However, the role of circ_0000140 in OSCC is still unknown. Methods: The expression of circ_0000140 and microRNA-182-5p (miR-182-5p) were assessed by quantitative realtime polymerase chain reaction (qRT-PCR). Also, cell proliferation, migration and invasion were measured by colony formation and transwell assays, respectively. Western blot (WB) analysis was used to test the levels of proliferation, metastasis and glycolysis metabolism-related proteins as well as cell division cycle 73 (CDC73) protein. Further, the extracellular acidification rate (ECAR) of cells was detected by the Seahorse XF Extracellular Flux Analyzer. The lactate acid level of cells was tested by Lactate Assay Kit. Moreover, dual-luciferase reporter was used to verify the interaction between miR-182-3p and circ_0000140 or CDC73, and RNA immunoprecipitation (RIP) assay was employed to further confirm the relationship between miR-182-3p and circ_0000140. In addition, mice xenograft models were built to measure the effect of circ_0000140 on OSCC tumor growth in vivo. Results: Circ_0000140 was lowly expressed in OSCC, and its overexpression hindered proliferation, migration, invasion and glycolysis metabolism in OSCC cells. MiR-182-5p could be sponged by circ_0000140, and its mimic could invert the suppression of circ_0000140 overexpression on OSCC progression. CDC73 could be targeted by miR-182-3p, and its silencing could reverse the inhibition of miR-182-3p inhibitor on OSCC progression. Further, overexpressed circ_0000140 reduced the OSCC tumor growth in vivo. Conclusions: Circ_0000140 might play an anti-cancer role in OSCC, which provided a novel target for clinical therapy of OSCC.

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“…Of all prognostic studies, one study investigated the prognostic value of tumor‐inhibitory circRNAs on DFS, 40 together with the other study assessed a combination of 10 circRNAs using The Cancer Genome Atlas clinical data for BC, 41 were both eliminated. Finally, 16 articles, 20‐35 including 4 individual studies on the diagnosis and 13 on the prognosis, were included for the subsequent meta‐analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Given that circRNAs are insensitive to nucleases and more stable than ordinary linear RNA, they are expected to be new biomarkers for monitoring various cancers 18,19 . And circRNAs are intensively reported to have the potential of the early diagnosis and prognostic prediction of BC as novel molecular biomarkers 20‐35 . However, the appraisals of their efficacy are commonly limited by the small sample size, high bias, and single‐center population of the already reported trials.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 And circRNAs are intensively reported to have the potential of the early diagnosis and prognostic prediction of BC as novel molecular biomarkers. [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] However, the appraisals of their efficacy are commonly limited by the small sample size, high bias, and single-center population of the already reported trials.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA profiling facilitates the diagnosis and prognostic monitoring of breast cancer: A pair‐wise meta‐analysis

Niu

Sha

et al. 2020

Clinical Laboratory Analysis

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Background As circular RNAs (circRNAs) have been found to significantly involve in the onset and progression of multiple malignant tumors including breast cancer (BC), this study aims at evaluating the diagnostic and prognostic values of circRNAs in this malady. Methods Available databases were thoroughly searched to collect studies on the diagnosis and/or prognosis of BC using circRNA profiling. The updated Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS‐2) tool and the Newcastle Ottawa Scale (NOS) were used to assess the underlying bias of included studies. Clinical characteristics of the studies were merged by the quantitative‐weighted integral method to obtain the combined effects. Results Sixteen studies were included, comprising 2438 BC cases and 271 noncancerous controls. The expression signature covered 24 circRNAs (down‐regulated: circ‐VRK1, hsa_circ_0068033, hsa_circ_103110, hsa_circ_104689, and hsa_circ_104821; up‐regulated: circAGFG1, hsa_circ_0001785, hsa_circ_0108942, hsa_circ_0001785, hsa_circ_006054, hsa_circ_100219, hsa_circ_406697, circEPSTI1, circANKS1B, circGFRA1, circ_0103552, CDR1‐AS, has_circ_001569, hsa_circ_001783, circFBXL5, circ_0005230, circAGFG1, circ‐UBAP2, and circ_0006528). The sensitivity and specificity of circRNAs in distinguishing BC patients from noncancerous controls were 0.65 and 0.68, and the corresponding area under the curve was 0.66. Survival analysis revealed that patients showing highly expressed oncogenic circRNAs were associated with increased mortality risks of BC in overall survival (univariate analysis: hazard ratio [HR] = 3.30, P = .000; multivariate analysis: HR = 3.07, P = .000), and disease‐free survival (HR = 8.26, P = .000). Stratified analysis based on circRNA expression status and control type also showed robust results. Conclusions Circular RNA profiling presents prominent diagnostic and prognostic values in BC, and can be rated as a promising tool facilitating its early diagnosis and survival.

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circFBXL5 promotes breast cancer progression by sponging miR‐660

Cited by 20 publications

References 18 publications

Circ‐PRMT5 promotes breast cancer by the miR‐509‐3p/TCF7L2 axis activating the PI3K/AKT pathway

Circ‐PRMT5 promotes breast cancer by the miR‐509‐3p/TCF7L2 axis activating the PI3K/AKT pathway

Circ_0000140 restrains the proliferation, metastasis and glycolysis metabolism of oral squamous cell carcinoma through upregulating CDC73 via sponging miR-182-5p

Circular RNA profiling facilitates the diagnosis and prognostic monitoring of breast cancer: A pair‐wise meta‐analysis

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