Circ‐PRMT5 promotes breast cancer by the miR‐509‐3p/TCF7L2 axis activating the PI3K/AKT pathway

Wu, Di; Jia, Hongyao; Zhang, Zhiru; Li, Sijie

doi:10.1002/jgm.3300

Cited by 26 publications

(18 citation statements)

References 35 publications

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“…High expression of circPRMT5 is associated with a poorer prognosis in BC patients. These findings are consistent with a recent study which the upregulation of circPRMT5 in breast cancer cells contributes to the aggressive phenotype by mediating the miR-509-3p/TCF7L2 axis to activate the PI3K/AKT pathway [32]. Apart from that, our study further demonstrated that circPRMT5 is indispensable for the invasion and migration capabilities of BC cells.…”

Section: Discussionsupporting

confidence: 93%

Circular RNA circPRMT5 is upregulated in breast cancer and is required for cell proliferation and migration

2021

Turk J Med Sci

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Objective. To evaluate the role of cyclic protein arginine methyltransferase 5 (circPRMT5) in the occurrence and development of breast cancer (BC). Methods. A total of 90 BC patients who underwent radical mastectomy and 40 age-matched healthy female controls were recruited in the Second People's Hospital of China Three Gorges University, Yichang Second People's Hospital from 2017 to 2020. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the expression levels of circPRMT5 in BC tissues, serum, normal breast cell line (MCF-10A) and BC cell line (T47D, MCF-7, BT549, Hs-578T and MDA-MB-231, MDA-MB-468). The associations between circPRMT5 expression level and age, tumor size, degree of differentiation, TNM stage, distant metastasis, estrogen receptor (ER) or progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) status were analyzed. BC cell lines with circPRMT5 knockdown or overexpression were subject to CCK-8 cell proliferation assay, and transwell cell invasion/migration assay. Results. CircPRMT5 expression in BC tissue was higher than that in adjacent normal breast tissue. Consistently, the expression level of circPRMT5 was also elevated in serum samples collected from BC patients when compared with healthy controls. And in multiple breast cancer cell lines, circPRMT5 was upregulated as compared to normal breast epithelial MCF-10A cells. CircPRMT5 expression level was correlated with tumor size, TNM stage, lymph node metastasis distant metastasis, but no correlation was observed with ER, PR, HER2 status. Overexpression of circPRMT5 promoted the proliferation, invasion and migration of MCF7 cells; while the knockdown of circPRMT5 inhibited cell proliferation, invasion and migration. Conclusion.CircPRMT5 seems to act as an oncogene in the progression of BC. Our data suggest that CircPRMT5 may be used as a biomarker for the diagnosis, prognosis evaluation and targeted therapy of breast cancer.

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Section: Discussionsupporting

confidence: 93%

Circular RNA circPRMT5 is upregulated in breast cancer and is required for cell proliferation and migration

2021

Turk J Med Sci

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“…Due to this closed-loop structure, to some extent, it helps circRNA resist degradation by RNase, which highlights the advantages of circRNAs as a stable molecular biomarker for diverse cancers [5][6][7]. circRNAs are also crucial regulators in cancer biology [8][9][10]. For example, circ-PRMT5 aggravates the malignant characters of breast cancer cells by activating the PI3K/AKT pathway and up-modulating TCF7L2 expression [8].…”

Section: Introductionmentioning

confidence: 99%

“…circRNAs are also crucial regulators in cancer biology [8][9][10]. For example, circ-PRMT5 aggravates the malignant characters of breast cancer cells by activating the PI3K/AKT pathway and up-modulating TCF7L2 expression [8]. So far, there are few researches on circ_0001287, and its role in NSCLC has yet to be studied.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ_0001287 inhibits the proliferation, metastasis, and radiosensitivity of non-small cell lung cancer cells by sponging microRNA miR-21 and up-regulating phosphatase and tensin homolog expression

Zhang

Feng

et al. 2021

Bioengineered

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As a type of non-coding RNA, circular RNA (circRNA) figures prominently in human cancer progression. Nonetheless, the expression, function, and regulatory mechanism of circ_0001287 in non-small cell lung cancer (NSCLC) remain obscure. In this work, quantitative real-time polymerase chain reaction (qRT-PCR) was implemented to quantify circ_0001287 and miR-21 expressions in NSCLC tissues and cells. The relationship between circ_0001287 expression and the clinicopathological parameters of NSCLC patients was examined. Cell counting kit-8 (CCK-8), 5-bromo-2©-deoxyuridine (BrdU), and Transwell experiments were conducted to detect the multiplication, migration, and invasion of NSCLC cells after circ_0001287 was overexpressed or knocked down. The survival of NSCLC cells was studied using colony formation experiment under different doses of radiation. RNA immunoprecipitation (RIP) experiment and luciferase reporter gene experiment verified the binding relationship between circ_0001287 and miR-21. Western blot was employed to examine the regulatory effects of circ_0001287 and miR-21 on phosphatase and tensin homolog (PTEN) expression. We reported that circ_0001287 expression was down-modulated in NSCLC tissues and cell lines. Besides, circ_0001287 low expression was associated with low differentiation and positive lymph node invasion of NSCLC. Circ_0001287 overexpression suppressed the multiplication, migration, invasion, and radioresistance of NSCLC cells, whereas circ_0001287 knockdown promoted the above phenotypes. Circ_0001287 could adsorb miR-21 and repress its expression, and indirectly up-modulate PTEN expression in NSCLC cells. Taken together, circ_0001287/miR-21/PTEN axis is probably involved in regulating NSCLC cell multiplication, metastasis, and radioresistance.

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“…7 The functions of circRNAs are coming into focus, and increasing evidence has shown that circRNAs play key regulatory roles in diverse cellular processes, such as cell proliferation, apoptosis, differentiation, and invasion. 8 In addition, several remarkable characteristics of circRNAs, including stability, 9 specificity, 10 conservatism, 11 and universality, 12 have been identified as potential biomarkers for diagnostics and as therapeutic targets in diseases, 13 such as ciRS-7 in Alzheimer's disease, 14 circ-PRMT5 in breast cancer, 15 hsa_circ_0000658 in osteosarcoma, 16 circRIMS1 in bladder cancer, 17 and circVMA21 in intervertebral disc degeneration. 18 Since 2015, the number of studies on circRNAs in OA has been increasing, and related publications have predominantly originated from China (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Biological roles and therapeutic potential of circular RNAs in osteoarthritis

Mao

Cao

Guo

et al. 2021

Molecular Therapy - Nucleic Acids

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Osteoarthritis (OA) is a common and disabling joint disorder that is mainly characterized by cartilage degeneration and narrow joint spaces. The regulatory functions of non-coding RNAs (long non-coding RNAs, microRNAs [miRNAs], and circular RNAs [circRNAs]) in OA progression have attracted considerable attention, and the function of circular RNAs in the context of OA has been an increasingly popular research topic in the last 6 years. Recent studies have reported that various circRNAs can delay or aggravate diverse aspects of the OA process, including extracellular matrix formation, apoptosis, proliferation, inflammation, and autophagy, via circRNA/miRNA/ mRNA pathways. Thus, circRNAs and related pathways are potential therapeutic targets for OA. Our review provides comprehensive information about circRNAs, including their biogenesis, functions, and characteristics, and it reveals their critical roles in the pathogenesis of OA via a large regulatory network of sponges. Considering their regulatory functions and characteristics, we hypothesize that circRNAs not only can be transferred through bodily fluids to serve as diagnostic biomarkers, but they can also be released from mesenchymal stem cell-derived exosomes and delivered to OA chondrocytes acting as therapeutic circRNAs. Further investigations of the in-depth molecular mechanisms of action of circRNAs in OA are expected to provide effective and safe OA treatment strategies.

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Circ‐PRMT5 promotes breast cancer by the miR‐509‐3p/TCF7L2 axis activating the PI3K/AKT pathway

Cited by 26 publications

References 35 publications

Circular RNA circPRMT5 is upregulated in breast cancer and is required for cell proliferation and migration

Circular RNA circPRMT5 is upregulated in breast cancer and is required for cell proliferation and migration

Circular RNA circ_0001287 inhibits the proliferation, metastasis, and radiosensitivity of non-small cell lung cancer cells by sponging microRNA miR-21 and up-regulating phosphatase and tensin homolog expression

Biological roles and therapeutic potential of circular RNAs in osteoarthritis

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