Circ_0000140 restrains the proliferation, metastasis and glycolysis metabolism of oral squamous cell carcinoma through upregulating CDC73 via sponging miR-182-5p

Guo, Jia; Su, Yuanyuan; Zhang, Meng

doi:10.1186/s12935-020-01501-7

Cited by 27 publications

(34 citation statements)

References 31 publications

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“…Here, we investigated the role of circ-KIAA0907 in OSCC. Consistent with previous study results [ 16 , 17 ], we confirmed that circ-KIAA0907 inhibited migration, invasion, glycolysis, and facilitated apoptosis of OSCC cells in vitro and OSCC tumor growth in vivo. Moreover, we also proposed that circ-KIAA0907 had an important positive regulatory effect on the radiosensitivity of OSCC cells.…”

Section: Discussionsupporting

confidence: 93%

“…Similar to this report, our study suggested that miR-96-5p was upregulated in OSCC and it also played the pro-metastasis role in OSCC. Consistent with the previous methods [ 16 , 17 ], the rescue experiments also were performed and the results showed that miR-96-5p reversed the inhibitory effect of circ-KIAA0907 on OSCC progression, indicating that miR-96-5p had a positively regulation on OSCC glycolysis, and had a negatively regulation on OSCC apoptosis and radiosensitivity. These results are a new finding, showing that circ-KIAA0907 targets miR-96-5p, a cancer-promoter, to hinder the progression of OSCC.…”

Section: Discussionsupporting

confidence: 86%

“…In previous studies, Peng et al verified that demonstrated that circ-KIAA0907 could interact with miR-31 by dual-luciferase reporter assay [ 16 ]. Guo et al used bioinformatics analysis to find that circ-KIAA0907 had binding sites with miR-182-5p, and confirmed that circ-KIAA0907 could sponge miR-182-5p using the dual-luciferase reporter assay and RIP assay [ 17 ]. Same as the previous method, we used bioinformatics analysis and validation of interaction relationship (dual-luciferase reporter assay and RIP assay) to indicate that circ-KIAA0907 could be used as a ceRNA of miR-96-5p.…”

Section: Discussionmentioning

confidence: 99%

“…Circ_0000140 is circularized from the KIAA0907 gene, so it is also known as circ-KIAA0907. In the previous studies, circ-KIAA0907 was found to significantly inhibit the proliferation, metastasis, and glycolytic metabolism of OSCC [ 16 , 17 ]. Therefore, exploring the new mechanism of circ-KIAA0907 regulating the progression of OSCC can provide new evidence for its potential as a therapeutic target for OSCC.…”

Section: Introductionmentioning

confidence: 99%

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Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis

Zhao

Zhang

et al. 2021

World J Surg Onc

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Background Circular RNA (circRNA) plays an important role in regulating cell biological function and has been shown to be involved in cancer progression, including oral squamous cell carcinoma (OSCC). Circ-KIAA0907 has been found to play an anti-cancer role in OSCC, so it is worth exploring more functions and new mechanisms of circ-KIAA0907 in OSCC progression. Methods Quantitative real-time PCR (qRT-PCR) was used to detect the expression of circ-KIAA0907, microRNA (miR)-96-5p, and unc-13 homolog C (UNC13C). Transwell assay, flow cytometry, and colony formation assay were employed to measure the migration, invasion, apoptosis, and radiosensitivity of cells. Besides, glucose uptake, lactate production, and extracellular acidification rate (ECAR) were determined to evaluate the glycolysis ability of cells. Dual-luciferase reporter assay and RIP assay were performed to confirm the interactions among circ-KIAA0907, miR-96-5p, and UNC13C. And RNA pull-down assay was used to verify the binding degree of miR-96-5p to its targets. Moreover, UNC13C protein level was examined using western blot (WB) analysis. OSCC xenograft models were constructed to perform in vivo experiments. Results Circ-KIAA0907 was a stability circRNA with lowly expression in OSCC. Overexpressed circ-KIAA0907 could inhibit migration, invasion, and glycolysis, while promoting apoptosis and radiosensitivity in OSCC cells. In the terms of mechanism, circ-KIAA0907 could sponge miR-96-5p to regulate UNC13C expression. MiR-96-5p overexpression could reverse the inhibitory effect of circ-KIAA0907 on OSCC progression, and UNC13C knockdown also could overturn the suppressive effect of miR-96-5p inhibitor on OSCC progression. Animal experiments revealed that circ-KIAA0907 could reduce the tumor growth of OSCC by regulating the miR-96-5p/UNC13C axis. Conclusion Our study suggests that circ-KIAA0907 restrains OSCC progression via the miR-96-5p/UNC13C axis, indicating that it may be a potential target for OSCC treatment.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis

Zhao

Zhang

et al. 2021

World J Surg Onc

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“… 27 Intriguingly, circ_0000140 has been identified as a tumor suppressor circRNA in oral squamous cell carcinoma by upregulating CDC73 via sequestration of miR-182-5p. 28 Furthermore, silencing of CDC73 could negate the suppressive effects of oe-circ_0000629 on cell migration, invasion, and proliferation of BC in our study. Basis on these findings, we exhibited that circ_0000629 serves as a miRNA sponge to restrain the BC development through the circ_0000629/miR-1290/CDC73 axis.…”

Section: Discussionsupporting

confidence: 49%

Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73

Wang

Luo

et al. 2021

CMAR

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Background Recent studies showed circular RNAs (circRNAs) played regulatory roles in bladder cancer (BC). However, the relevance of circ_0000629, a newly identified circRNA, has not been determined yet. We aimed to characterize the function of circ_0000629 in BC and the relevant mechanism. Methods First, we downloaded circRNA-related microarrays GSE147985 and GSE92675 from the GEO database, followed by a validation in our clinically obtained samples. We then overexpressed circ_0000629 in T24 and SW780 cells and evaluated the effects of circ_0000629 on BC cell proliferatory, apoptotic, and metastatic abilities. We further detected the subcellular localization of circ_0000629 in T24 and SW780 cells by the fractionation and export assay and FISH experiments. Integrated microarray analyses and bioinformatics website prediction were utilized to screen out the downstream microRNA (miRNA)/mRNA. The effects of miR-1290 and CDC73 on BC cell growth and metastasis was verified by functional rescue experiments. In addition, mice xenografts were built to measure the effect of circ_0000629 on tumor growth in vivo. Results Circ_0000629 and CDC73 were reduced, and miR-1290 was significantly overexpressed in BC tissues and cells. Moreover, circ_0000629 significantly inhibited the development and metastasis of BC cells, but further overexpression of miR-1290 or knockdown of CDC73 attenuated the inhibitory effect of circ_0000629 on BC cells. Circ_0000629 localized in the cytoplasm and regulated CDC73 expression by sponging miR-1290. Further, overexpressed circ_0000629 reduced the BC tumor growth in vivo. Conclusion Circ_0000629 promotes the expression of CDC73 by competitively binding to miR-1290, thereby inhibiting the growth and metastasis of BC cells.

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The role and mechanism of circ‐BNC2 on the malignant progression of oral squamous cell carcinoma

et al. 2023

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BackgroundCircular RNAs (circRNAs) play a key part in the progression of oral squamous cell carcinoma (OSCC). However, the role of circ‐BNC2 (circRNA ID hsa_circ_0086414) in OSCC progression is still unclear.MethodsPlasmid transfection was used to induce overexpression of circ‐BNC2. RNA expression of circ‐BNC2, microRNA‐142‐3p (miR‐142‐3p) and GNAS complex locus (GNAS) was detected by quantitative real‐time polymerase chain reaction. Protein expression was assessed by western blot assay or immunohistochemistry assay. Cell proliferation was investigated by 3‐(4,5‐dimethylthazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, colony formation assay and flow cytometry analysis. Cell migratory and invasive abilities and cell apoptosis were assessed by transwell assay and flow cytometry analysis, respectively. Oxidative stress was evaluated by superoxide dismutase activity detection assay, lipid peroxidation malondialdehyde assay and cellular reactive oxygen species assay. The binding relationship between miR‐142‐3p and circ‐BNC2 or GNAS was proved by dual‐luciferase reporter assay and RNA immunoprecipitation assay. The impacts of circ‐BNC2 overexpression on tumor growth in vivo were unveiled by a xenograft mouse model assay.ResultsCirc‐BNC2 expression was downregulated in OSCC tissues and cells when compared with adjacent healthy tissues and normal human oral keratinocytes. Circ‐BNC2 overexpression repressed the proliferation, migration and invasion of OSCC cells but induced cell apoptosis and oxidative stress. Additionally, circ‐BNC2 overexpression inhibited tumor growth in vivo. Furthermore, circ‐BNC2 bound to miR‐142‐3p, and miR‐142‐3p targeted GNAS. MiR‐142‐3p mimic attenuated circ‐BNC2 overexpression‐mediated effects on the proliferation, migration, invasion, apoptosis and oxidative stress of OSCC cells. The regulation of miR‐142‐3p in OSCC cell tumor properties involved GNAS. Further, circ‐BNC2 introduction promoted GNAS expression by inhibiting miR‐142‐3p.ConclusionCirc‐BNC2 suppressed OSCC malignant progression by upregulating GNAS expression in a miR‐142‐3p‐dependent manner, which suggested that circ‐BNC2 might be a novel target for OSCC therapy.

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Circ_0000140 restrains the proliferation, metastasis and glycolysis metabolism of oral squamous cell carcinoma through upregulating CDC73 via sponging miR-182-5p

Cited by 27 publications

References 31 publications

Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis

Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis

Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73

The role and mechanism of circ‐BNC2 on the malignant progression of oral squamous cell carcinoma

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