Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation

Yue, Binglin; Zhang, Chunlei; Fang, Xingtang

doi:10.1002/jcb.25901

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…The development of bovine skeletal muscle is a complex process involving prenatal and postnatal patterns, which are mainly due to all muscle fiber formation, the muscle fiber size increases and new muscle fibers regenerate, respectively (Buckingham et al, 2003). In our research, two developmental stages were chosen: the embryonic 90-day stage, a prenatal generation when a large amount of muscle progenitor cell proliferation occurs; and the adult 24-month-old stage, during which myofibers are well established (Hocquette, 2010; Yue et al, 2017). Considering the differential expression of RNAs from these two stages, these RNAs might be associated with myogenesis, particularly lncRNAs and miRNAs that are known to have more developmental stage-specific expression patterns than protein-coding genes (Guttman and Rinn, 2012).…”

Section: Discussionmentioning

confidence: 99%

Characterization of lncRNA–miRNA–mRNA Network to Reveal Potential Functional ceRNAs in Bovine Skeletal Muscle

Yue

Liu

et al. 2019

Front. Genet.

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There is growing evidence that non-coding RNAs are emerging as critical regulators of skeletal muscle development. In order to reveal their functional roles and regulatory mechanisms, we constructed a lncRNA–miRNA–mRNA network according to the ceRNA (competitive endogenous RNA) theory, using our high-throughput sequencing data. Subsequently, the network analysis, GO (Gene Ontology) analysis, and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis were performed for functional annotation and exploration of lncRNA ceRNAs. The results uncovered a scale-free characteristics network which exhibited high functional specificity for bovine skeletal muscle development: co-expression lncRNAs were significantly enriched in muscle development related biological processes and the Wnt signaling pathway. Furthermore, GSEA (Gene Set Enrichment Analysis) indicated that the risk score has a tendency to associate with myogenesis, and differentially expressed RNAs were validated by qPCR, further confirming the credibility of our network. In summary, this study provides insights into lncRNA-mediated ceRNA function and mechanisms in bovine skeletal muscle development and will expand our understanding of lncRNA biology in mammals.

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Section: Discussionmentioning

confidence: 99%

Characterization of lncRNA–miRNA–mRNA Network to Reveal Potential Functional ceRNAs in Bovine Skeletal Muscle

Yue

Liu

et al. 2019

Front. Genet.

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“…Muscle-specific miRNAs, including miR-1 9 , miR-133 10 , miR-206 11 , miR-208 12 , and miR-499 13 , have been considered key regulators with different targets during muscle development. Moreover, ubiquitously expressed miRNAs, such as miR-128 14 , miR-27 15 , miR-155 16 , miR-378 17 , miR-486 18 , miR-29 7 , miR-29b 19 , miR-34b 20 , miR-152 21 , and miR-660 22 , have been found to be crucial factors affecting the formation of skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

Chromatin accessibility is associated with the changed expression of miRNAs that target members of the Hippo pathway during myoblast differentiation

Zhou

Xiang

et al. 2020

Cell Death Dis

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miRNAs reportedly participate in various biological processes, such as skeletal muscle proliferation and differentiation. However, the regulation of differentially expressed (DE) miRNAs and their function in myogenesis remain unclear. Herein, miRNA expression profiles and regulation during C2C12 differentiation were analyzed in relation to chromatin states by RNA-seq, ATAC-seq, and ChIP-seq. We identified 19 known and nine novel differentially expressed miRNAs at days 0, 1, 2, and 4. The expression of the differentially expressed miRNAs was related to the chromatin states of the 113 surrounding open chromatin regions defined by ATAC-seq peaks. Of these open chromatin regions, 44.25% were colocalized with MyoD/MyoG binding sites. The remainder of the above open chromatin regions were enriched with motifs of the myoblast-expressed AP-1 family, Ctcf, and Bach2 transcription factors (TFs). Additionally, the target genes of the above differentially expressed miRNAs were enriched primarily in muscle growth and development pathways, especially the Hippo signaling pathway. Moreover, via combining a loss-of-function assay with Q-PCR, western blotting, and immunofluorescence, we confirmed that the Hippo signaling pathway was responsible for C2C12 myoblast differentiation. Thus, our results showed that these differentially expressed miRNAs were regulated by chromatin states and affected muscle differentiation through the Hippo signaling pathway. Our findings provide new insights into the function of these differentially expressed miRNAs and the regulation of their expression during myoblast differentiation.

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“…The mature miRNA with the RISC, in most cases, binds to the 3'-untranslated region (3'-UTR) of the target mRNAs to elicit translation inhibition and/or degradation of the target transcripts (Figure 1 ) 18 . Research has shown that miRNAs play critical roles in a myriad of biological processes, including proliferation 19 , differentiation 20 , 21 , and apoptosis 22 of cells, immune response 23 , and angiogenesis 24 .…”

Section: Biogenesis and Functions Of Mirnas And Lncrnasmentioning

confidence: 99%

Decoding Noncoding RNAs: Role of MicroRNAs and Long Noncoding RNAs in Ocular Neovascularization

Zhang¹,

Cai²,

Jia³

et al. 2017

Theranostics

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Ocular neovascularization is a pathological sequel of multiple eye diseases. Based on the anatomical site into which the abnormal neovessels grow, ocular neovascularization can be categorized into corneal neovascularization, choroidal neovascularization, and retinal neovascularization. Each category is intractable, and may lead to blindness if not appropriately treated. However, the current therapeutic modalities, including laser photocoagulation, vitrectomy surgery, and anti-VEGF drugs, raise concerns due to limited efficacy, damage on retinal parenchyma and vasculature, and the patients' unresponsiveness to the treatments. Therefore, the in-depth study on pathogenesis of and the search for novel therapeutic targets to the ocular neovascularization are needed. During the last 10 years or so, a large number of literatures have emerged indicating a critical role of noncoding RNAs, particularly microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), in the pathogenesis and regulation of the ocular neovascularization. This review summarizes the current understanding of the biosynthesis and functions of the miRNAs and lncRNAs, the regulation of the miRNAs and lncRNAs in neovascular eye diseases, as well as the roles of these noncoding RNAs in the disease models of ocular neovascularization, in the hope that it could provide clues for the pathogenesis of and molecular targets to the ocular neovascularization.

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Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation

Cited by 8 publications

References 38 publications

Characterization of lncRNA–miRNA–mRNA Network to Reveal Potential Functional ceRNAs in Bovine Skeletal Muscle

Characterization of lncRNA–miRNA–mRNA Network to Reveal Potential Functional ceRNAs in Bovine Skeletal Muscle

Chromatin accessibility is associated with the changed expression of miRNAs that target members of the Hippo pathway during myoblast differentiation

Decoding Noncoding RNAs: Role of MicroRNAs and Long Noncoding RNAs in Ocular Neovascularization

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