microRNA as a new immune-regulatory agent in breast milk

Kosaka, Nobuyoshi; Izumi, Hirohisa; Sekine, Kazunori; Ochiya, Takahiro

doi:10.1186/1758-907x-1-7

Cited by 478 publications

(541 citation statements)

References 24 publications

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“…We speculated that isolated mEVs can be used for various applications. Human-milk-derived mEVs have been shown to modulate the functionality of immune cells [12,31]. Similar to human mEVs, bovine mEVs may have a biological function, as described previously [16].…”

Section: Resultsmentioning

confidence: 92%

Biocompatibility of highly purified bovine milk‐derived extracellular vesicles

Somiya

Yoshioka²,

Ochiya³

2018

J of Extracellular Vesicle

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197

168

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Extracellular vesicles (EVs) deliver biologically active cargos from donor cells to recipient cells for intercellular communication. Since the existence of RNA cargo was discovered, EVs have been considered to be useful drug-delivery systems. Specifically, EVs from bovine milk (mEV) are one of the most promising platforms, since bovine milk is a scalable source of EVs for mass production. However, it is still difficult to isolate pure EVs from bovine milk owing to the complexity of raw materials. Furthermore, the biocompatibility and immunotoxicity of mEVs are still unclear. In this study, we developed a new method for isolating bovine milk-derived EVs by employing acid treatment and ultracentrifugation. Isolated mEVs are spherical in shape, measure 120 nm in diameter and contain typical EV marker proteins, such as tetraspanins. Compared with the previously reported method, our method can isolate purer mEVs. When mEVs are contacted with the mouse macrophage cell line Raw264.7, mEVs are readily taken up by the cells without a cytotoxic effect, suggesting that mEVs can deliver the cargo molecules into cells. While systemic administration of mEVs into mice resulted in the absence of systemic toxicity, certain types of cytokines were slightly induced. No anaphylaxis effect was observed after serial administration of mEVs in mice. Thus, mEVs isolated using our method are well tolerated in vivo and may be useful for the drug-delivery application.

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Section: Resultsmentioning

confidence: 92%

Biocompatibility of highly purified bovine milk‐derived extracellular vesicles

Somiya

Yoshioka²,

Ochiya³

2018

J of Extracellular Vesicle

Self Cite

197

168

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“…Studies conducted by several research groups have confirmed the presence of miRNAs in various body fluids in humans, including serum [49][50][51], plasma [49,50], saliva [52], urine [53], milk [54], cerebrospinal fluid [55] and seminal fluid [56]. In cancer, there is a distinct relationship between the type of biological material and the original location of the neoplasm (e.g.…”

Section: Extracellular Mirnamentioning

confidence: 99%

miRNAs as Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer: Current Perspectives

Florczuk¹,

Szpechcinski²,

Chorostowska‐Wynimko³

2017

Targ Oncol

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Lung cancer is the most common cancer worldwide. Up to 85% of lung cancer cases are diagnosed as nonsmall cell lung cancer (NSCLC). The effectiveness of NSCLC treatment is expected to be improved through the implementation of robust and specific biomarkers. MicroRNAs (miRNAs) are small, non-coding molecules that play a key role in the regulation of basic cellular processes, including differentiation, proliferation and apoptosis, by controlling gene expression at the post-transcriptional level.

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“…MicroRNAs milk derivatives are stable under harsh conditions such as freeze-thaw cycles and low pH (pH 1); this is of importance for premature newborns, VLBW, and hospitalized infants, who usually receive freezer-stored breast milk (even donated). Low pH stability suggests that miRNAs can be tolerated by newborn's gastrointestinal milieu and could be absorbed into the small intestine directly influencing the gut mucosal immunity and therefore microbiome (11,67). The analysis of miRNAs profile, in human breast milk, with respect to innate immunity could represent a strategy to treat critical premature newborns.…”

Section: Nutrition: the Role Of Micrornasmentioning

confidence: 99%

Innate Immunity and Human Milk MicroRNAs Content: A New Perspective for Premature Newborns

et al. 2017

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Context: The premature newborns are prone to develop both early onset and late onset neonatal sepsis. The major causes of this phenomenon rely on the immaturity of the immune system, which has reduced capability to respond adequately to pathogens. Evidence Acquisition: Titles and abstracts of previous papers were scanned before reading the full-text, in order to retrieve appropriate information. The databases used for searching were PubMed, Cochrane, and Embase for articles published before 1st of July, 2016. Secondary search for articles cited in reference lists were identified by the primary search. This review focused on neonatal sepsis incidence and the associated immune response with regards to microRNAs of human milk as a new microelement that enables regulation of innate immunity functions. Results: Since human milk is a valuable source of microRNAs, a better understanding of its content will open a new therapeutic avenue for the clinical management of infectious diseases affecting premature newborns. The variation in miRNAs quantity in human milk needs to be considered. Mother's milk can have different amounts of miRNAs and the identification of a microMilk batch richer of miRNAs can be a nutrition intervention method for modulating innate immunity in clinical management of premature newborns. Conclusions: Routine translation of the microMilk concept for neonatal intensive care unit (NICU), in the management of premature newborns could be a way of defending premature newborns and Very Low Birth Weight (VLBW) infants from both early and late sepsis.

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microRNA as a new immune-regulatory agent in breast milk

Cited by 478 publications

References 24 publications

Biocompatibility of highly purified bovine milk‐derived extracellular vesicles

Biocompatibility of highly purified bovine milk‐derived extracellular vesicles

miRNAs as Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer: Current Perspectives

Innate Immunity and Human Milk MicroRNAs Content: A New Perspective for Premature Newborns

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