miRNAs as Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer: Current Perspectives

Florczuk, Mateusz; Szpechcinski, Adam; Chorostowska‐Wynimko, Joanna

doi:10.1007/s11523-017-0478-5

Cited by 91 publications

(67 citation statements)

References 196 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Growing evidence has revealed that miRNAs play crucial roles in tumor development. 17 miR-23a-3p was found downregulated in mucosal melanoma. 18 In addition, miR-23a-3p overexpression inhibited mucosal melanoma cell malignancy and tumorigenicity.…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Kang

Shi

et al. 2019

BioFactors

View full text Add to dashboard Cite

Non‐small cell lung cancer (NSCLC) accounts for about 80% of lung cancers worldwide. In recent years, importance of noncoding RNAs including long noncoding RNA and microRNA in regulating tumor progression has been appreciated. Abnormally expression of DiGeorge syndrome critical region gene 5 (DGCR5) was found in multiple human cancers but its function in NSCLC is largely unknown. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) was conducted to explore DGCR5 expression level in NSCLC. Bioinformatic analyses were conducted to explore the targets of DGCR5. Cell counting kit‐8 assay, wound‐healing assay, and transwell invasion assay were performed to analyze functions of DGCR5. RT‐qPCR revealed that DGCR5 expression in NSCLC cells was significantly lower than in normal cell. DGCR5 overexpression suppresses NSCLC cell growth, migration, and invasion. Online algorithms found EPH receptor B6 (EPHB6) and DGCR5 contains same miR‐211‐5p binding region. The predicted connections were further validated by luciferase activity reporter assay. Recue experiments showed DGCR5 regulates NSCLC cell behaviors via targeting miR‐211‐5p/EPHB6. These findings collectively identified DGCR5/miR‐211‐5p/EPHB6 triple axis in NSCLC, which may novel understanding regarding the tumorigenesis of NSCLC.

show abstract

Section: Discussionmentioning

confidence: 93%

“…Therefore, identifying miRNA/mRNA that has a connection with DGCR5 will advance our understanding of the DGCR5 roles in NSCLC. Growing evidence has revealed that miRNAs play crucial roles in tumor development . miR‐23a‐3p was found downregulated in mucosal melanoma .…”

Section: Discussionmentioning

confidence: 99%

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Kang

Shi

et al. 2019

BioFactors

View full text Add to dashboard Cite

show abstract

“…It is known that aberrant expression or mutations of miRNAs are crucial for the development of cancer . These important roles of miRNAs in human cancers provide novel biomarkers or targets for cancer diagnosis and treatments . For example, miR‐21 was reported to be capable of conferring chemoresistance of advanced pancreatic cancer .…”

Section: Discussionmentioning

confidence: 99%

“…30,31 These important roles of miRNAs in human cancers provide novel biomarkers or targets for cancer diagnosis and treatments. 32 For example, miR-21 was reported to be capable of conferring chemoresistance of advanced pancreatic cancer. 33 miR-328 was reported to be highly up-regulated after resveratrol treatment in osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 99%

Propofol inhibits pancreatic cancer proliferation and metastasis by up‐regulating miR‐328 and down‐regulating ADAM8

Gao

Zhang

2019

Basic Clin Pharma Tox

View full text Add to dashboard Cite

Propofol is commonly used for anaesthesia during surgery, and accumulating evidence has demonstrated that propofol is associated with tumour suppression. For example, propofol down‐regulates the expression of vascular endothelial growth factor to inhibit pancreatic cancer malignancy. However, deeper insights into its underlying mechanism are needed. In this study, we treated pancreatic cell lines Panc1 and Bxpc3 with or without propofol. Cell proliferation, migration and invasion were evaluated using 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and transwell assays. Real‐time polymerase chain reaction was used to measure RNA expression levels. Luciferase assay was performed to determine the transcriptional activity of microRNAs (miRNAs). We found that propofol significantly reduced the proliferation, migration and invasion of pancreatic cancer cells compared to untreated cells. By testing the changes in miRNAs after propofol treatment, propofol was shown to strikingly enhance the expression of miR‐328. Luciferase assays demonstrated that propofol repressed the transcriptional activity of miR‐328, while a disintegrin and metalloproteinase 8 (ADAM8) was a direct target of miR‐328. Knockdown of miR‐328 or ADAM8 led to significantly decreased cell growth and viability. Our results implicate that propofol inhibits pancreatic cancer growth and metastasis by enhancing miR‐328 which targets ADAM8.

show abstract

“…MicroRNAs (miRNAs) are one type of small non‐coding RNAs which can modulate gene expression at post‐transcriptional level in various cancers . In NSCLC, many miRNAs have been revealed to be involved in the cancer carcinogenesis by functioning as tumor suppressors or oncogenes, and miRNAs are potential diagnostic biomarkers and therapeutic targets in NSCLC . Recently, the important roles of the lncRNA‐miRNA‐mRNA regulatory network and a protein‐protein interaction network have also been identified in the development of NSCLC .…”

Section: Introductionmentioning

confidence: 99%

LncRNA SNHG16 promotes non‐small cell lung cancer development through regulating EphA2 expression by sponging miR‐520a‐3p

Chen

Song

2020

Thoracic Cancer

View full text Add to dashboard Cite

Background Recent evidence has found that lncRNA small nucleolar RNA host gene 16 (SNHG16) was associated with cell carcinogenesis in NSCLC. Here, we further investigated the precise functions and mechanisms of SNHG16 in NSCLC progression. Methods The expression of SNHG16, microRNA (miR)‐520a‐3p and EPH Receptor A2 (EphA2) was measured using quantitative real‐time polymerase chain reaction and western blot, respectively. Cell proliferation was determined using 3‐(4, 5)‐dimethylthiahiazo (−z‐y1)‐3, 5‐di‐phenytetrazoliumromide (MTT) assay. The migrated and invaded cells were measured by Transwell assay. Flow cytometry was used to detect apoptotic cells. The interaction between miR‐520a‐3p and SNHG16 or EphA2 was confirmed using a dual‐luciferase reporter assay. Results We found that SNHG16 was upregulated in NSCLC tissues and cell lines, knockdown of SNHG16 inhibited cell proliferation, migration, invasion and induced apoptosis in vitro as well as suppressed tumor growth in vivo. MiR‐520a‐3p directly bound to SNHG16 and miR‐520a‐3p, and SNHG16 acted as a ceRNA in regulating EphA2 through competitively binding to miR‐520a‐3p. Additionally, rescue assay exhibited the anticancer activity mediated by SNHG16 knockdown on NSCLC could be reversed by miR‐520a‐3p inhibition or EphA2 overexpression. Conclusion SNHG16 promoted NSCLC development by regulating the miR‐520a‐3p/EphA2 axis, suggesting novel insights for the pathogenesis of NSCLC and new potential therapeutic targets for the treatment of NSCLC. Key points Knockdown of SNHG16 inhibited NSCLC cell proliferation, migration, invasion and induced apoptosis in vitro as well as suppressed tumor growth in vivo. SNHG16 directly interacted with miR‐520a‐3p. EphA2 was a target of miR‐520a‐3p. SNHG16 could regulate the expression of EphA2 by binding to miR‐520a‐3p. SNHG16 promoted NSCLC development by regulating the miR‐520a‐3p/EphA2 axis.

show abstract

miRNAs as Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer: Current Perspectives

Cited by 91 publications

References 196 publications

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Propofol inhibits pancreatic cancer proliferation and metastasis by up‐regulating miR‐328 and down‐regulating ADAM8

LncRNA SNHG16 promotes non‐small cell lung cancer development through regulating EphA2 expression by sponging miR‐520a‐3p

Contact Info

Product

Resources

About