LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Kang, Mafei; Shi, Jing; Li, Bihui; Luo, Meiqing; Xu, Shaohua; Liu, Xiuli

doi:10.1002/biof.1539

Cited by 26 publications

(18 citation statements)

References 19 publications

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“…We used the GEO database to select mRNAs that were downregulated in SOC, identified potential lncRNA-miRNA-mRNA interaction networks based on the presence of specific binding sites, and reconstructed a comprehensive ceRNA network. Several recent studies demonstrated that ceRNA-based mechanisms may operate in all types of carcinoma [5,6,[17][18][19][20][21][22][23]. In the present study, among the miRNAs found to be downregulated in SOC, hsa-miR-195-5p and hsa-miR-497-5p were predicted to bind to LINC00284.…”

Section: Discussionsupporting

confidence: 54%

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

et al. 2020

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The low survival rate associated with serous ovarian carcinoma (SOC) is largely due to the lack of relevant molecular markers for early detection and therapy. Increasing experimental evidence has demonstrated that long noncoding RNAs (lncRNAs) are involved in cancer initiation and development, and a competitive endogenous RNA (ceRNA) hypothesis has been formulated. Therefore, the characterization of new lncRNA and lncRNA-related networks is crucial for early diagnosis and targeted therapy of SOC. Data on lncRNAs, mRNAs, and miRNAs with differential expression in SOC, compared to normal ovarian tissue, were obtained from the Gene Expression Omnibus (GEO) database. Data on lncRNA expression and clinical data in SOC were obtained from The Cancer Genome Atlas (TCGA). lncRNA-miRNA interactions were predicted by the miRBase database. Different online tools, i.e., TargetScan, RNA22, miRmap, microT, miRanda, StarBase, and PicTar, were cooperatively utilized to predict the mRNAs targeted by miRNAs. The plugin of BiNGO in Cytoscape and KOBAS 3.0 were used to conduct the functional and pathway enrichment analyses. The lncRNA, miRNAs, and mRNAs identified to be expressed at statistically significant and different levels between SOC and healthy fallopian tube tissues were further validated using qRT-PCR. A total of 4 lncRNAs (LINC00284, HAGLR, HCAT158, and BLACAT1) and 111 mRNAs were found to be upregulated in SOC tissues compared to normal tissues, based on the GEO database. LINC00284 was found to be highly expressed in SOC, in association with the upregulation of the transcription factor SOX9. The high LINC00284 expression was associated with poor prognosis and proved to be an independent risk factor in patients with SOC, based on TCGA database. The qRT-PCR validation results closely recapitulated the expression profiles and prognostic scores of the aforementioned bioinformatic analyses. The LINC00284-related ceRNA network was found to be associated with SOC carcinogenesis by biofunctional analysis. In conclusion, the LINC00284-related ceRNA network may provide valuable information on the mechanisms of SOC initiation and progression. Importantly, LINC00284 proved to be a new potential prognostic biomarker for SOC.

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Section: Discussionsupporting

confidence: 54%

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

et al. 2020

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“…Considering that lncRNAs are reported to take part in the regulation of gene expression via many kinds of mechanisms, such as suppression of gene translation, degradation of microRNAs, the elevation of chromatin modification, modulation of protein activity, and so on (Jarroux, Morillon, & Pinskaya, 2017). DGCR5 has been discovered to attend to the regulation of microRNA-22-3p (Dong, Wang, Jin, Zeng, & Pan, 2018), microRNA-346/kruppel-like factor (KLF14; Wang, Liu et al, 2019), microRNA-873-5p/tumor suppressor candidate 3 (TUSC3; Luo et al, 2018), microRNA-221-5p/EPH receptor 6 (EPHB6; Kang et al, 2019) and miR-195 (Tang, Shan, & Zeng, 2019). We can propose that some miRNAs are involved in the regulatory mechanisms of DGCR5 on Raf1/ERK1/2 and Wnt/βcatenin pathways in hepatocellular carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Retracted:Propofol exerts anticancer activity on hepatocellular carcinoma cells by raising lncRNA DGCR5

Sun

2019

Journal Cellular Physiology

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Hepatocellular carcinoma is one of the most fatal cancers worldwide. Propofol is an intravenous anesthetic extensively used in clinical. Herein, we tested the anticancer activity of propofol on hepatocellular carcinoma, along with the internal molecular mechanism related to lncRNA DiGeorge syndrome critical region gene 5 (DGCR5).Followed by propofol stimulation, hepatocellular carcinoma Huh-7 and HepG2 cell viability, proliferation, migration, invasion, and apoptosis were tested, respectively.Then, DGCR5 expression levels in hepatocellular carcinoma tissues and cells were measured. sh-DGCR5 was transfected to silence DGCR5 expression. Subsequently, the influence of DGCR5 silence on propofol caused Huh-7 and HepG2 cell viability loss, proliferation inhibition, migration and invasion suppression, apoptosis induction, as well as Raf1/ERK1/2 and Wnt/β-catenin pathways inactivation were assessed, respectively. We discovered that propofol declined Huh-7 and HepG2 cell viability, proliferation, migration and invasion, but increased cell apoptosis. DGCR5 had a relatively lower expression level in hepatocellular carcinoma tissues and cells.Propofol elevated DGCR5 expression in Huh-7 and HepG2 cells. Increased expression of DGCR5 was connected with the anticancer activity of propofol on Huh-7 and HepG2 cells. Besides, propofol repressed Raf1/ERK1/2 and Wnt/β-catenin pathways through elevating DGCR5 expression. In conclusion, the anticancer activity of propofol on hepatocellular carcinoma was verified in this study. Propofol repressed hepatocellular carcinoma Huh-7 and HepG2 cell growth and metastasis at least by elevating DGCR5 and hereafter inactivating Raf1/ERK1/2 and Wnt/β-catenin pathways.

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“…In other words, miR‐124‐3p acted as an inhibitory factor in tumors. Additionally, a great number of studies have manifested that lncRNA could exert ceRNA function to regulate miRNA . Accordingly, we discovered that LINC01410 modulated miR‐124‐3p as a ceRNA to promote CCA development.…”

Section: Discussionmentioning

confidence: 97%

LINC01410 promotes cell proliferation and migration of cholangiocarcinoma through modulating miR‐124‐3p/SMAD5 axis

Jiang

Wang

Zhu

et al. 2020

The Journal of Gene Medicine

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Background: Cholangiocarcinoma (CCA) is generally associated with high incidence and poor prognosis. Nowadays, increasing experimental data demonstrate that long non-coding RNA (lncRNA) plays an indispensable role in tumor occurrence.Nevertheless, the specific mechanism of lncRNA is not clear in CCA. Methods:The relative expressions of lncRNAs, miRNAs, and mRNAs were detected by real-time quantitative PCR (RT-qPCR). CCK8 and colony formation assays were applied to examine cell proliferation ability in CCA. Transwell assay was conducted to measure the migration and invasion capabilities of CCA cells.Nuclear and cytoplasmic separation assay was implemented to figure out the location of LINC01410. Luciferase reporter assay, RIP and RNA pull-down assays were applied to certify the molecular bindings. Western blot was applied to detect the protein level.Results: The high expression of LINC01410 was proved in CCA tissues and CCA cell lines. Also, CCA patients with high LINC01410 level presented poor prognosis. LINC01410 deficiency impeded cell proliferation, migration and invasion in HuCCT1and RBE cell lines. What's more, LINC01410 interacted with miR-124-3p. Meanwhile, SMAD5 targeted and inhibited by miR-124-3p. SMAD5 expression was enhanced by LINC01410.Conclusion: LINC01410 facilitates cell proliferation, migration and invasion through miR-124-3p/SMAD5 axis.

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LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Cited by 26 publications

References 19 publications

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

Construction and Investigation of an LINC00284-Associated Regulatory Network in Serous Ovarian Carcinoma

Retracted:Propofol exerts anticancer activity on hepatocellular carcinoma cells by raising lncRNA DGCR5

LINC01410 promotes cell proliferation and migration of cholangiocarcinoma through modulating miR‐124‐3p/SMAD5 axis

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