MicroRNA-375 Is Downregulated in Pancreatic Cancer and Inhibits Cell Proliferation In Vitro

Abstract

MicroRNAs (miRNAs) have emerged as important regulators in the development of pancreatic cancer and may be a valuable therapeutic application. Aberrant expression of microRNA-375 (miR-375) has been reported to be involved in development and progression in various types of cancers, but few studies have been conducted to determine its relationship with pancreatic cancer. Quantitative RT-PCR was used to detect the levels of miR-375 expression in pancreatic cancer tissue samples and cells. The cell growth rate of pancreatic cancer cells transfected with pre-miR-375 was examined by CCK8 assay. The effects of miR-375 on cell cycle and apoptosis were assessed by flow cytometry analyses. In this study, we found that the expression levels of miR-375 was significantly lower in pancreatic cancer tissues compared with nontumorous tissues. We found that miR-375 level in pancreatic cancer was associated with lymph nodes metastasis and clinical stage, and did not correlated with any other factors such as sex, age, position, tumor size, or histological grading. The CCK8 assay showed that that cells transfected with pre-miR-375 inhibited cell proliferation in Panc-1 and SW1990 cells. Flow cytometry analysis indicated that upregulation of miR-375 led to an increase in the percentage of cells in G0/G1 phase in the cell cycle distribution and induced cell apoptosis. Our research suggested that miR-375 has potential as a novel suppressor gene in pancreatic cancer and its downregulation may promote the progression of pancreatic cancer. Overexpression of miR-375 impacts cell proliferation, cell cycle distribution, and apoptosis of pancreatic cancer cells, miR-375 may play an important role in the novel therapeutic strategy for pancreatic cancer.

miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1

“…In addition, miR-375 is involved in maintaining mouse pancreatic α and β cell mass [24]. Previous studies indicated that the expression of miR-375 is down-regulated in human pancreatic cancer, squamous cell carcinoma, liver cancer, adenocarcinoma of the esophagus and gastric carcinomas, ectopic expression of miR-375 disturbed the proliferation of these cancer cells [25,26,27]. Based on the above findings that YAP1 was the target of miR-375 (Fig.…”

MicroRNA dysregulation in uveal melanoma: a new player enters the game

“…By base-pairing with the complementary sites in the 3′untranslated region (3′UTR) of the mRNA, miRNAs regulate target genes by increasing mRNA decay or repressing translation [19–21]. Growing evidences indicate that miRNAs control many cellular processes, including cell development, differentiation, proliferation and apoptosis [22–27].…”

MIRNAS: Early Prognostic Biomarkers for Type 2 Diabetes Mellitus?

“…Also, many of these miRNAs have also been identified as prognostic markers for other diseases (like, cancer) and other complications associated with T2DM (including atherosclerosis, renal injury, non-alcoholic fatty liver disease, etc.). For example, in addition to being identified as a marker for T2DM, miR-375 is downregulated in pancreatic cancer and overexpression of miR-375 impacts cell proliferation, cell cycle distribution and apoptosis of pancreatic cancer cells [67]. Additionally, miR-375 also targets cancer-related proteins -caveolin1, JAK2 and YAP1 [57].…”