MicroRNA‑199a‑5p suppresses cell proliferation, migration and invasion by targeting ITGA3 in colorectal cancer

Abstract

as a member of the integrin family, integrin α3β1 (iTGa3) has been linked to intercellular communication and serves an important role in the signaling among cells and the extracellular matrix. Microrna (mir)-199a-5p has been demonstrated to be related to the pathogenesis and progression of multiple malignant diseases. However, the biological functions of mir-199a-5p and iTGa3 in colorectal cancer (crc) have rarely been reported. The aim of the present study was to explore the roles of mir-199a-5p and iTGa3 in crc. immunohistochemistry staining and western blotting were applied to detect the protein expression of iTGa3 in crc tissues and cells. reverse transcription-quantitative Pcr was performed to investigate the expression of mir-199a-5p and iTGa3 mrna. HcT-116 cells were transfected with mir-199a-5p mimics, mimics control, short hairpin rna targeting iTGa3, or pcdna-iTGa3 for the functional experiments. dual luciferase reporter assay was applied to confirm whether mir-199a-5p targeted the 3' untranslated region (3'uTr) of iTGa3. The MTT, Transwell and wound healing assays were used to evaluate the proliferation, invasion and migration of CRC cells. Immunofluorescence assay was used to monitor the epithelial-mesenchymal transition (eMT) biomarker expression. The results demonstrated downregulation of mir-199a-5p and upregulation of iTGa3 in crc tissues and cell lines. mir-199a-5p mimics and knockdown of iTGa3 suppressed the proliferation, invasion and migration of crc cells. Bioinformatics analysis and luciferase reporter assay indicated that mir-199a-5p targeted the 3'uTr of the iTGa3 transcript, and overexpression of iTGa3 reversed the tumor-suppressive effects of mir-199a-5p elevation. In addition, the immunofluorescence assay suggested that mir-199a-5p mimics suppressed the eMT of crc cells, whereas the overexpression of iTGa3 restored this effect. in conclusion, mir-199a-5p may act as a tumor suppressor by targeting and negatively regulating iTGa3 in crc.

Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

“…In this study, the ECM-receptor interaction was substantially enriched in 3 DEMs, including ITGA3, ITGA4 , and COL4A5 . These three genes were more closely related to diseases ( 64 – 66 ), but studies have found that ITGA3, ITGA4 , and COL4A1 may be involved in the composition of adipocyte extracellular matrix and the differentiation of adipocytes ( 67 ).…”

MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2

“…An image of each scratch was captured under a light microscope. Subsequently, cells were incubated in RPMI-1640 medium containing 1% FBS (26). After culturing for 24 h at 37˚C, images of the scratches were captured again.…”

Changes in Exosomal miRNA Composition in Thyroid Cancer Cells after Prolonged Exposure to Real Microgravity in Space

“…Our chosen target inhibits proliferation, migration, and invasion in colorectal cancer by negatively regulating integrin α3β1 (ITGA3), which has been linked to intercellular communication and serves an important role in the signaling among cells and the extracellular matrix. In addition, it was suggested that miR-199a-5p overexpression suppresses the EMT of colorectal cancer cells, whereas the overexpression of ITGA3 restores this effect [ 137 ]. The same holds true by looking at another miR199a-5p direct target, ROCK1 (Rho-associated coiled coil-containing protein kinase 1), Zhu and colleagues demonstrated in their study that microRNA-199a-5p was significantly downregulated in colorectal cancer cell lines and tissue samples and was associated with a poor prognostic phenotype.…”