Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

Bai, Yang; Li, Xupeng; Chen, Zongchang; Li, Jingsheng; Tian, Hongshan; Ma, Yan; Raza, Sayed Haidar Abbas; Shi, Baoming; Han, Xiaodong; Luo, Yuzhu; Hu, Jiang; Wang, Jiqing; Liu, Xiu; Li, Shaobin; Zhao, Zhidong

doi:10.3389/fvets.2021.788316

Cited by 6 publications

(18 citation statements)

References 81 publications

(89 reference statements)

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“…To analyze the DEGs between the two groups, the R software package DESeq2 [ 8 ] (1.20.0) was used to determine mRNA expression levels. The resulting p -values were adjusted (padj) using the Benjamin-Hochberg method for controlling the false discovery rate, and padj ≤ 0.05 and log2FC (fold change) ≥ 1 or ≤ −1 were set as the threshold for significantly differential expression.…”

Section: Methodsmentioning

confidence: 99%

“…Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of DEG and DEM targets were implemented using the R package ClusterProfiler [ 8 , 9 ] (3.8.1). Differences were considered statistically significant at padj ≤ 0.05.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous of studies have shown that both microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) play precise regulatory roles in adipocyte proliferation and differentiation [ 4 , 7 ]. LncRNAs can regulate various biological processes through epigenetic, transcriptional, and post-transcriptional mechanisms [ 8 , 9 ]. MiRNAs primarily bind to the 3′-untranslated region sequence of an mRNA, leading to translation inhibition or gene splicing [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Regulation of Yak Preadipocyte Differentiation and Proliferation by LncFAM200B and ceRNA Regulatory Network Analysis

Ran

Yang

Luo

et al. 2022

Cells

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The positive regulatory role of lncFAM200B in differentiation and lipid deposition in yak intramuscular preadipocytes has been demonstrated in our previous study. However, the regulatory mechanisms remain unclear. In this study, we aimed to produce complete mRNA and microRNA (miRNA) profiles after adenovirus-mediated lncFAM200B overexpression in yak preadipocytes using high-throughput sequencing. We constructed a competing endogenous RNA (ceRNA) network with lncFAM200B as the core and identified the functions of the selected target miRNA during cell proliferation and differentiation. We obtained 118 differentially expressed genes (DEGs) after lncFAM200B overexpression, 76 of which were up-regulated, including Notch signaling members NOTCH3, DTX3L, and HES4, and 42 DEGs were down-regulated, including genes related to the cell cycle (CCNA2, BUB1, CDC20, TOP2A, and KIF20A). Additionally, many ubiquitin-mediated proteolysis pathway members were also significantly up-regulated (BUA7, PML, TRIM21, and TRIM25). MiRNA sequencing showed that 13 miRNAs were significantly up-regulated, and 12 miRNAs were down-regulated. Among them, 29 targets of 10 differentially expressed miRNAs (DEMs) were differentially expressed, including miR-152-FBXO33, miR-6529a-TRIM21, miR-148c-NOTCH3, and the miR-6529b-HES4 axis. We further verified that overexpression and inhibition of miR-6529a can inhibit and promote, respectively, the proliferation and differentiation of preadipocytes. Taken together, our study not only revealed the regulatory network of lncFAM200B during yak preadipocytes differentiation but also laid a foundation for elucidating the cause for lower intramuscular fat content in yaks at the molecular level.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Regulation of Yak Preadipocyte Differentiation and Proliferation by LncFAM200B and ceRNA Regulatory Network Analysis

Ran

Yang

Luo

et al. 2022

Cells

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“…We selected nine signaling pathways for miRNA target gene enrichment, which are related to lipid metabolism. Then we intersect the genes enriched in the signaling path with the mRNAs expressed differently in our previous sequencing results ( Bai et al, 2021 ), and use these mRNAs and miRNAs to build a miRNA-mRNA interaction network.…”

Section: Methodsmentioning

confidence: 99%

“…This study aimed to further explore the molecular mechanism of miRNAs regulating the synthesis of unsaturated fatty acids in bovine adipocytes. High-throughput sequencing was used to systematically identify miRNAs and mRNAs ( Bai et al, 2021 ) that were differentially expressed in bovine adipocytes after interference with the ACSL1 gene and linked the mRNAs related to fatty acid metabolism with miRNAs to construct a miRNA-mRNA interaction network. The results provide new ideas and directions for the regulation of unsaturated fatty acid metabolism in bovine adipocytes.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of microRNAs related to unsaturated fatty acid synthesis by interfering bovine adipocyte ACSL1 gene

Bai²,

Li³

et al. 2022

Front. Genet.

Self Cite

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Long-chain fatty acyl-CoA synthase 1 (ACSL1) plays a vital role in the synthesis and metabolism of fatty acids. The proportion of highly unsaturated fatty acids in beef not only affects the flavor and improves the meat’s nutritional value. In this study, si-ACSL1 and NC-ACSL1 were transfected in bovine preadipocytes, respectively, collected cells were isolated on the fourth day of induction, and then RNA-Seq technology was used to screen miRNAs related to unsaturated fatty acid synthesis. A total of 1,075 miRNAs were characterized as differentially expressed miRNAs (DE-miRNAs), of which the expressions of 16 miRNAs were upregulated, and that of 12 were downregulated. Gene ontology analysis indicated that the target genes of DE-miRNAs were mainly involved in biological regulation and metabolic processes. Additionally, KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis identified that the target genes of DE-miRNAs were mainly enriched in metabolic pathways, fatty acid metabolism, PI3K-Akt signaling pathway, glycerophospholipid metabolism, fatty acid elongation, and glucagon signaling pathway. Combined with the previous mRNA sequencing results, several key miRNA-mRNA targeting relationship pairs, i.e., novel-m0035-5p—ACSL1, novel-m0035-5p—ELOVL4, miR-9-X—ACSL1, bta-miR-677—ACSL1, miR-129-X—ELOVL4, and bta-miR-485—FADS2 were screened via the miRNA-mRNA interaction network. Thus, the results of this study provide a theoretical basis for further research on miRNA regulation of unsaturated fatty acid synthesis in bovine adipocytes.

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Effects of high-grain diet feeding on fatty acid profiles in milk, blood, muscle, and adipose tissue, and transcriptional expression of lipid-related genes in muscle and adipose tissue of dairy cows

Cui

Lin

Lai

et al. 2022

Preprint

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Background High-grain (HG) diets affect lipid metabolism in the liver and mammary tissue of dairy cows, but its effects on muscle and adipose tissue have not been wide evaluated. Thus, the aim of this study is to clarify this issue. Methods Twelve Holstein cows were randomly divided into two groups: conventional diet group (CON, n = 6) and the HG diet (n = 6). On day7 of week4, rumen fluid was sampled to measure pH, milk was sampled to measure components, and blood was sampled to measure biochemical parameters and fatty acid composition. After the experiment, cows were slaughtered to collect muscle and adipose tissue for fatty acid composition and transcriptome analysis. Results HG feeding decreased the ruminal pH, milk’s fat content and long-chain fatty acid proportion (P < 0.05) and increased the proportion of short- and medium-chain fatty acids in the milk (P < 0.05) as compared with CON diets. The concentrations of blood cholesterol, low-density lipoprotein, and polyunsaturated fatty acids in the HG cows were lower than those in CON cows (P < 0.05). In muscle tissue, HG feeding tended to increase the triacylglycerol (TG) concentration (P < 0.10). Transcriptome analysis revealed changes in the biosynthesis of the unsaturated fatty acids pathway, the regulation of lipolysis in the adipocytes pathway, and the PPAR signalling pathway. In adipose tissue, HG feeding increased the concentration of TG and monounsaturated fatty acids (P < 0.05). At the transcriptome level, the fatty acid biosynthesis pathway, linoleic acid metabolism pathway, and PPAR signalling pathway were activated. Conclusion HG feeding leads to subacute rumen acidosis (SARA) and a decreased milk fat content. The fatty acid profiles in the milk and plasma of dairy cows were changed by HG feeding. In muscle and adipose tissue, HG feeding increased TG concentration and up-regulated the expression of genes related to adipogenesis, while down-regulated the expression of genes related to lipid transport. These results complement our knowledge of the fatty acid composition of muscle and adipose tissue in dairy cows and expand our understanding of the mechanisms by which HG diets affect lipid metabolism in muscle and adipose tissue.

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Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

Cited by 6 publications

References 81 publications

Molecular Regulation of Yak Preadipocyte Differentiation and Proliferation by LncFAM200B and ceRNA Regulatory Network Analysis

Molecular Regulation of Yak Preadipocyte Differentiation and Proliferation by LncFAM200B and ceRNA Regulatory Network Analysis

Transcriptional analysis of microRNAs related to unsaturated fatty acid synthesis by interfering bovine adipocyte ACSL1 gene

Effects of high-grain diet feeding on fatty acid profiles in milk, blood, muscle, and adipose tissue, and transcriptional expression of lipid-related genes in muscle and adipose tissue of dairy cows

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