Molecular Regulation of Yak Preadipocyte Differentiation and Proliferation by LncFAM200B and ceRNA Regulatory Network Analysis

Abstract

The positive regulatory role of lncFAM200B in differentiation and lipid deposition in yak intramuscular preadipocytes has been demonstrated in our previous study. However, the regulatory mechanisms remain unclear. In this study, we aimed to produce complete mRNA and microRNA (miRNA) profiles after adenovirus-mediated lncFAM200B overexpression in yak preadipocytes using high-throughput sequencing. We constructed a competing endogenous RNA (ceRNA) network with lncFAM200B as the core and identified the functions of the selected target miRNA during cell proliferation and differentiation. We obtained 118 differentially expressed genes (DEGs) after lncFAM200B overexpression, 76 of which were up-regulated, including Notch signaling members NOTCH3, DTX3L, and HES4, and 42 DEGs were down-regulated, including genes related to the cell cycle (CCNA2, BUB1, CDC20, TOP2A, and KIF20A). Additionally, many ubiquitin-mediated proteolysis pathway members were also significantly up-regulated (BUA7, PML, TRIM21, and TRIM25). MiRNA sequencing showed that 13 miRNAs were significantly up-regulated, and 12 miRNAs were down-regulated. Among them, 29 targets of 10 differentially expressed miRNAs (DEMs) were differentially expressed, including miR-152-FBXO33, miR-6529a-TRIM21, miR-148c-NOTCH3, and the miR-6529b-HES4 axis. We further verified that overexpression and inhibition of miR-6529a can inhibit and promote, respectively, the proliferation and differentiation of preadipocytes. Taken together, our study not only revealed the regulatory network of lncFAM200B during yak preadipocytes differentiation but also laid a foundation for elucidating the cause for lower intramuscular fat content in yaks at the molecular level.

Unraveling genomic diversity and positive selection signatures of Qaidam cattle through whole‐genome re‐sequencing

“…In the North Chinese cattle, the nucleotide diversity of Qaidam cattle is higher than that of the other four breeds. It is speculated that this may be due (Purfield et al, 2012). In study, European taurine cattle, Eurasian taurine cattle and East Asian taurine cattle with a higher degree of commercialization showed a higher overall number and length of ROHs, indicating that these breeds were subjected to high-intensity artificial selection and had a higher degree of inbreeding.…”

“…Regarding meat production performance, BCKDHB is involved in the fatty acid synthesis pathway and is selected among Chinese Holstein cattle, Australian Angus cattle and Snow Dragon black cattle (de Las Heras‐Saldana et al., 2019; Herman et al., 2010; Yan et al., 2020; Zhao et al., 2020). HES4 plays a role in the molecular regulation of yak adipocyte differentiation and fat deposition (Ran et al., 2022). PDE3B is involved in lipid metabolism in Nelore cattle and is associated with intramuscular fat deposition (Diniz et al., 2019).…”

“…A run of homozygosity (ROH) is a continuous homozygous region in the DNA sequence of diploid organisms. The presence of long ROHs results from inbreeding, while shorter ROHs reflect the influence of ancient ancestors (Purfield et al, 2012). To evaluate the ROH patterns of Qaidam cattle and other cattle breeds/ populations, we divided the length of ROHs into four size classes: 0.5-1, 1-2, 2-4 and >4 Mb (Figure 2c and Table S5).…”

pCMLM: Genome Wide Association Study of Body Size Traits in Multiple Regions of Yak Based on the Provided Compressed Mixed Linear Model

“…Yak ( Bos grunniens ) is a large livestock species unique to the Qinghai-Tibet Tibetan Plateau and the surrounding Hengduan Mountains, providing a basic resource for the livelihood of plateau farmers and herders [Ran H, et al 2022]. There are 12 breeds of yak in China due to the different geographical and climatic environments, ecological conditions, grassland types, feeding levels, breeding levels, and social and economic structures in the main producing areas.…”