MicroRNA-195 inhibits non-small cell lung cancer cell proliferation, migration and invasion by targeting MYB

Zhou, Yunyun; Tian, Linwei; Wang, Xicai; Ye, Lianhua; Zhao, Genming; Yu, Ming; Guanjian, Li; Lei, Yonghong; Huang, Yunchao

doi:10.1016/j.canlet.2014.01.019

Cited by 92 publications

(78 citation statements)

References 37 publications

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“…For instance, Yongchun et al (27) reported that miR-195 could decrease cell proliferation, migration and invasion of NSCLC via the proto-oncogene Myb. You et al (28) observed that miR-132 blocks the migration and invasion of NSCLC cells through targeting the EMT regulator ZEB2.…”

Section: Discussionmentioning

confidence: 99%

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

et al. 2016

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Abstract. Emerging evidence suggests that microRNAs (miRs) play critical roles in the development and progression of non-small cell lung cancer (NSCLC). In a previous study, the present authors demonstrated that miR-154 acts as a tumor suppressor in NSCLC; however, its underlying molecular mechanism and target in NSCLC remain poorly understood. In the present study, ectopic expression of miR-154 remarkably suppressed cell migration and invasion in NSCLC cells.Zinc finger E-box binding homeobox 2 (ZEB2) was identified as a direct target of miR-154 in NSCLC cells. Furthermore, overexpression of miR-154 could decrease the expression of ZEB2 at the messenger RNA and protein levels. Ectopic expression of miR-154 also increased the levels of E-cadherin, an epithelial marker, and decreased the levels of vimentin, a mesenchymal marker, which contributed to suppress epithelial-mesenchymal transition (EMT) and to inhibit cell migration and invasion. In addition, downregulation of ZEB2 exerted similar effects to those caused by miR-154 overexpression on NSCLC cell migration and invasion, while upregulation of ZEB2 could significantly reverse the inhibitory effects on migration and invasion caused by miR-154 on NSCLC cells. These findings demonstrated that miR-154 inhibited migration and invasion of NSCLC cells by regulating EMT through targeting ZEB2, suggesting that miR-154 may be a potential anticancer therapeutic target for NSCLC.

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Section: Discussionmentioning

confidence: 99%

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

et al. 2016

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“…MYB affects diverse processes, including proliferation, invasion and migration, through activation of the transcription of target genes, such as cyclooxygenase-2, B-cell lymphoma 2 and c-Myc (20). It has been reported that miR-195 targets MYB in non-small cell lung cancer cells (9). The present study demonstrated that miR-195 directly targeted the 3'-UTR of the MYB gene and inhibited its expression in HeLa cells, suggesting that the deregulation of miR-195-mediated MYB inhibition may serve as a general mechanism in the development of a variety of cancers (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…miR-195 has been reported to be downregulated in the tumor tissues and serum of cancer patients. miR-195 is involved in multiple processes during cancer development, including cell proliferation, migration, invasion and metastasis (7)(8)(9)(10)(11)(12). However, the function of miR-195 in cervical cancer development is unknown.…”

Section: Introductionmentioning

confidence: 99%

microRNA-195 inhibits the proliferation, migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression

Zhang

et al. 2015

Oncology Letters

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Abstract. The functions of microRNAs (miRNA/miR) in the development of cervical cancer remain largely undefined. The present study investigated the role of miR-195 in cervical cancer development. The expression of miR-195 mimics in the cervical cancer HeLa cell line significantly decreased the cell proliferation, migration and invasion capacities in vitro. Using miRNA target prediction algorithms and reporter assays, cyclin D2 (CCND2) and v-myb avian myeloblastosis viral oncogene homolog (MYB) were identified as direct targets of miR-195. Moreover, miR-195 repressed the expression of CCND2 and MYB in the HeLa cells at the mRNA and protein levels. Finally, the expression of miR-195 was downregulated in cervical cancer tissues compared with normal tissues. Together, these data suggest that miR-195 is a tumor suppressor in cervical cancer. IntroductionAs the third most commonly diagnosed cancer and the fourth leading cause of cancer mortality in females globally, cervical cancer accounts for 9% of the total new cases of cancer and 8% of the total cancer-related fatalities among females in 2008. In total, >85% of these cases and fatalities occur in developing countries (1,2). It has been reported that human papillomavirus (HPV) infection is involved in the development of >90% of cases (3). However, people who have had HPV infections do not necessarily develop cervical cancer, indicating that other risk factors also contribute to the development of cervical cancer (3). Despite a number of studies and resources aimed at elucidating the molecular mechanisms of cervical cancer, the exact initiation and progression processes remain unclear.microRNAs (miRNA/miR) are 20-25-nucleotide RNAs that regulate gene expression at the post-transcriptional level by binding to target mRNAs for translational repression or mRNA cleavage (4). Recent studies have shown that miRNAs are grossly deregulated in a variety of human cancers, including cervical cancer, and are vital in the processes of cancer initiation, progression and metastasis (5,6). Studies have shown that miR-195 acts as a putative tumor suppressor in a variety of cancers, including hepatocellular carcinoma (7,8), non-small cell lung cancer (9), human glioblastoma (10), breast cancer (11) and colorectal cancer (12). miR-195 has been reported to be downregulated in the tumor tissues and serum of cancer patients. miR-195 is involved in multiple processes during cancer development, including cell proliferation, migration, invasion and metastasis (7-12). However, the function of miR-195 in cervical cancer development is unknown.In the present study, the expression of miR-195 was detected in cervical cancer tissues and the functions of miR-195 in the cervical cancer HeLa cell line was studied in order to investigate the role of miR-195 in cervical cancer. Materials and methodsCell culture. HeLa cells were obtained from the American Type Culture Collection and maintained in Dulbecco's modified Eagle's medium (DMEM; Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented wit...

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“…microRNAs (miRNAs) have been identified to play critical regulatory roles in cancer proliferation [18], invasion [19], prognosis [20] and other biology processes in a variety of research disciplines [21,22]. miRNAs indirectly regulate the target genes by binding their 3 0 -UTR.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of miR‐638 promotes invasion and proliferation by regulating SOX2 and induces EMT in NSCLC

Xia

Liu

et al. 2014

FEBS Letters

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Edited by Tamas DalmayKeywords: miR-638 SRY (sex determining region Y)-box 2 Proliferation Invasion Epithelial-to-mesenchymal transition Non-small-cell lung cancer a b s t r a c t Aberrant expression of microRNAs has been shown to regulate the biological processes of lung cancer cells. However, the role of miR-638 in the development of NSCLC is still unclear. In this study, low miR-638 and high SOX2 were shown to be associated with tumor size and metastasis of NSCLC patients. Downregulated miR-638 could promote cell invasion and proliferation, while high miR-638 expression reversed the effect. Furthermore, miR-638 could regulate SOX2 by directly binding to its 3 0 -UTR. Silencing of SOX2 by siRNA partially abolished the enhancement of cell invasion and proliferation induced by downregulated miR-638. Aberrant miR-638 expression could modulate the expression levels of markers of epithelial-to-mesenchymal transition. Our results indicate that miR-638 may play a pivotal role in the development of NSCLC.

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MicroRNA-195 inhibits non-small cell lung cancer cell proliferation, migration and invasion by targeting MYB

Cited by 92 publications

References 37 publications

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

miR-154 inhibits migration and invasion of human non-small cell lung cancer by targeting ZEB2

microRNA-195 inhibits the proliferation, migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression

Downregulation of miR‐638 promotes invasion and proliferation by regulating SOX2 and induces EMT in NSCLC

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