microRNA-195 inhibits the proliferation, migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression

Du, Xinjie; Li, Lin; Zhang, Lijun; Jiang, Jie

doi:10.3892/ol.2015.3541

Cited by 39 publications

(26 citation statements)

References 20 publications

(27 reference statements)

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“…miRNA has previously been identified as an important factor in cell proliferation, division, metastasis or apoptosis in cervical cancer (20). In the present study, it was demonstrated that miR-10-5p was significantly downregulated in cervical cancer compared with normal cervical cells.…”

Section: Discussionsupporting

confidence: 61%

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

Zhai¹,

Li²,

Lan³

et al. 2017

Exp Ther Med

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Abstract. The aim of the present study was to investigate the effect and mechanism of microRNA (miR)-10a-5p in human cervical cancer. The expression level of miR-10-5p in cervical cancer lines was assessed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In cervical cancer HeLa and SiHa cells, miR-10-5p was ectopically overexpressed by lentiviral transduction. Brain-derived neurotrophic factor (BDNF) was then overexpressed in HeLa and SiHa cells to evaluate its selective effect on miR-10-5p in cervical cancer modulation. The targeting of miR-

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Section: Discussionsupporting

confidence: 61%

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

Zhai¹,

Li²,

Lan³

et al. 2017

Exp Ther Med

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“…The function of miR-195 in CC has been widely probed, and it is certified that miR-195 inhibits CC cell proliferation, invasion and migration by targeting related genes, while the knockdown of miR-195 has the opposite impact on cell processes [3,6,24,25]. In this study, we further demonstrated that miR-195-5p could lessen CC cell proliferation and invasion ability via targeting MMP14.…”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andsupporting

confidence: 49%

“…Cervical carcinoma (CC) is the third most prevalent cancer and the fourth mortality leading cancer in females worldwide, for which the treatment remains a problem [3]. The aim of the present study was to find new biomarkers and hereby improve the current therapy methods for CC.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous researches have demonstrated that miRNA expression level is closely related to human cancer progression and metastasis. Previous studies have shown that various miRNAs are abnormally expressed in CC tissues, and miR-195 is one of them [3,6]. Some studies have been done to elucidate the role of miR-195 in CC cells, and it is believed that miR-195 functions as a tumor suppressing miRNA in CC [2].…”

Section: Introductionmentioning

confidence: 99%

“…Over 50 million new patients are diagnosed with CC yearly around the world, especially in developing countries, which account for over 80% of all worldwide cases [1][2][3]. Conventionally, radiotherapy and chemotherapy individually or both have been used for CC treatment.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

Ren

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical cancer (CC) and their roles on cell proliferation and invasion profile of CC cells through TNF signaling pathway in CC. Methods: Microarray analysis, gene set enrichment analysis (GSEA) and DAVID were used to analyze differentially expressed miRNAs, mRNAs and signaling pathways. MiR-195-5p and MMP14 expression levels in CC cell were determined by qRT-PCR. Western blot was employed to measure MMP14 and TNF signaling pathway-relating protein level. Luciferase reporter system was used to confirm the targeting relationship between MMP14 and miR-195-5p. Cell proliferation and invasion was respectively deeded by CCK8, transwell. In vivo experiment was carried out to study the impact of MMP14 and miR-195-5p on CC development in mice. Results: The microarray analysis and the results of qRT-PCR determined that miR-195-5p was under-expressed and MMP14 was over-expressed in CC cells. GSEA and DAVID analysis showed that TNF signaling pathway was regulated by miR-195-5p/MMP14 and activated in cervical carcinoma cells. The miR-195-5p and MMP14 have a negative regulation relation. In vivo experiment found that down-regulated MMP14 and up-regulated miR-195-5p suppressed the tumor development. Conclusion: Our results suggest that MMP14 is a direct target of miR-195-5p, and down-regulated MMP14 and up-regulated miR-195-5p suppressed proliferation and invasion of CC cells by inhibiting TNF signaling pathway.

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Retracted: Long non‐coding RNA XIST promotes osteosarcoma progression by targeting YAP via miR‐195‐5p

Yang

Wang

et al. 2018

J of Cellular Biochemistry

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The lncRNA XIST (X inactive-specific transcript) is an oncogenic lncRNA that is present in various malignant tumors; however, its role and molecular mechanisms in osteosarcoma (OS) progression remain unclear. In the current study, 40 pairs of OS tissues and matched adjacent non-tumor tissues were collected. qRT-PCR was conducted to investigate the differences in XIST expression in tissues and OS cell lines. The proliferation, invasion, and EMT status of OS cells after transfection were assessed with WST-1 assays, Transwell assays, and Western blot analysis, respectively. Whether miR-195-5p was a direct downstream target of XIST was verified by both bioinformatics target gene prediction and dual-luciferase report analysis. A mouse model was established to evaluate tumor proliferation in vivo. Our results demonstrated that XIST expression was significantly upregulated in OS tissues and cell lines and negatively correlated with clinical prognosis. XIST knockdown inhibited cancer cell proliferation and invasion in vitro, inhibited the EMT of OS cells in vitro, and suppressed subcutaneous tumor growth in vivo. Further analysis demonstrated that XIST regulated YAP expression by functioning as a competing endogenous RNA that sponged miR-195-5p in OS cells. XIST directly interacted with miR-195-5p and decreased the binding of miR-195-5p to the YAP 3'UTR, which suppressed the degradation of YAP mRNA by miR-195-5p. In conclusion, this work demonstrates that lncRNA XIST enhances OS cancer cell proliferation and invasion in part through the miR-195-5p/YAP pathway. Therefore, lncRNA XIST might be a promising therapeutic target for OS.

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microRNA-195 inhibits the proliferation, migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression

Cited by 39 publications

References 20 publications

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

Retracted: Long non‐coding RNA XIST promotes osteosarcoma progression by targeting YAP via miR‐195‐5p

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