Retracted: Long non‐coding RNA XIST promotes osteosarcoma progression by targeting YAP via miR‐195‐5p

Abstract

The lncRNA XIST (X inactive-specific transcript) is an oncogenic lncRNA that is present in various malignant tumors; however, its role and molecular mechanisms in osteosarcoma (OS) progression remain unclear. In the current study, 40 pairs of OS tissues and matched adjacent non-tumor tissues were collected. qRT-PCR was conducted to investigate the differences in XIST expression in tissues and OS cell lines. The proliferation, invasion, and EMT status of OS cells after transfection were assessed with WST-1 assays, Transwell assays, and Western blot analysis, respectively. Whether miR-195-5p was a direct downstream target of XIST was verified by both bioinformatics target gene prediction and dual-luciferase report analysis. A mouse model was established to evaluate tumor proliferation in vivo. Our results demonstrated that XIST expression was significantly upregulated in OS tissues and cell lines and negatively correlated with clinical prognosis. XIST knockdown inhibited cancer cell proliferation and invasion in vitro, inhibited the EMT of OS cells in vitro, and suppressed subcutaneous tumor growth in vivo. Further analysis demonstrated that XIST regulated YAP expression by functioning as a competing endogenous RNA that sponged miR-195-5p in OS cells. XIST directly interacted with miR-195-5p and decreased the binding of miR-195-5p to the YAP 3'UTR, which suppressed the degradation of YAP mRNA by miR-195-5p. In conclusion, this work demonstrates that lncRNA XIST enhances OS cancer cell proliferation and invasion in part through the miR-195-5p/YAP pathway. Therefore, lncRNA XIST might be a promising therapeutic target for OS.

Long noncoding RNA LINC00968 promotes endothelial cell proliferation and migration via regulating miR‐9‐3p expression

“…Long noncoding RNAs (lncRNAs) are defined as >200 nucleotides in length with limit or without protein‐coding ability . LncRNAs have emerged as important regulators in all aspects of fields of biology through regulation at the chromatin organization, posttranscriptional, and transcriptional levels .…”

LncRNA NCRNA00173 is down-regulated in pediatric osteosarcoma and suppresses cell metastasis of osteosarcoma cells through regulating PI3k/Akt pathway

“…Compared with lowly expressed of XIST group, the group of highly expressed has shorter overall survival in OS. 25,26 In this research, we found that NCRNA00173 was down-regulated in OS tissues and notably relevant with TNM stage and metastasis. Meanwhile, the OS and average survival time of low NCRNA00173 expression ware shorter than high NCRNA00173 expression within 5 years.…”

Aberrantly expressed long non‐coding RNAs in air pollution‐induced congenital defects

“…[17][18][19] Accumulating evidence suggested that lncRNAs are deregulated in most, if not all, types of human diseases, including tumours, cardiovascular diseases, autoimmune diseases and congenital abnormalities. [20][21][22][23][24] Mechanistically, lncRNAs modulate gene expression at transcriptional and post-transcriptional levels and participate in the regulation of a plethora of molecular, cellular and tissue processes, including DNA synthesis, chromatin modification, cell proliferation, apoptosis, differentiation and organ development. [25][26][27][28] Emerging evidence also suggested that lncRNAs could participate in the pathogenesis of orthopaedic diseases, such as intervertebral disc degeneration, osteoporosis, osteosarcoma and osteoarthritis.…”