MicroRNA-1287-5p promotes ferroptosis of osteosarcoma cells through inhibiting GPX4

Abstract

Figure S1. Overexpression of miR-1287-5p induces ferroptosis and inhibition of human osteosarcoma cells. (A) Relative LPO and MDA levels at 96 h after the miR-1287-5p mimic (100 nmol/L) transfection (n=6). (B-C) The releases of 5-HETE and 15-HETE to the medium from the miR-1287-5p mimic (100 nmol/L)transfected cell (n=6). (D) Intracellular GSH levels (n=6). (E) Quantification of cystine uptake levels (n=6). (F) Relative protein level of GPX4 in human osteosarcoma cell lines treated with the miR-1287-5p mimic or control for 96 h at indicating doses (n=6). (G) Relative level of intracellular ROS was determined by DCFH-DA staining at 96 h after the miR-1287-5p mimic transfection with indicating doses (n=6). (H) Cell death ratio at 96 h after the miR-1287-5p mimic transfection with indicating doses (n=6). The data are expressed as the mean ± SD and a P value < 0.05 was regarded as statistical significance. n.s. indicates no statistical significance.

Comprehensive Analysis of a Ferroptosis-Related lncRNA Signature for Predicting Prognosis and Immune Landscape in Osteosarcoma

“…As research progresses, the implications of ferroptosis in the development, progression, and multi-drug resistance of osteosarcoma are becoming better known, providing new avenues for treating osteosarcoma and overcoming chemoresistance (74). For illustration, it was found that MicroRNA-1287-5p promotes ferroptosis in osteosarcoma cells by inhibiting GPX4, but its expression is downregulated in human osteosarcoma relative to controls (78). These results suggest that ferroptosis may be regulated by different genes or non-coding RNAs in osteosarcoma, but the expression of these regulators differs in osteosarcoma and normal tissues.…”

“…KDM4A expression was significantly upregulated in OS tumor tissues relative to normal tissues, while KDM4A knockdown promoted ferroptosis in osteosarcoma cells, limited pulmonary metastasis of osteosarcoma, and could act synergistically with cisplatin ( 77 ). MicroRNA-1287-5p promotes ferroptosis in osteosarcoma cells by inhibiting GPX4, but its expression is downregulated in human osteosarcoma relative to controls ( 78 ). These results suggest that ferroptosis may be regulated by different genes or non-coding RNAs in osteosarcoma, but the expression of these regulators differs in osteosarcoma and normal tissues.…”

Advances in the Biological Functions and Mechanisms of miRNAs in the Development of Osteosarcoma

“… 165 Recently, it was also found that miRNA-1287-5p could inhibit the progression of osteosarcoma cells by inhibiting GPX4 to promote ferroptosis in osteosarcoma cells. 166 However, there are few literatures about the mechanism of miRNAs regulating ferroptosis in osteosarcoma, and a lot of research is needed to make up for this deficiency in the future.…”

A graphical journey through iron metabolism, microRNAs, and hypoxia in ferroptosis

“…Thus, microRNAs targeting Keap1 and activating Nrf2 can be considered as anti-ferroptotic [ 6 ]. Pro-ferroptotic microRNAs directly target the SLC7A11/GPX4 system and facilitate lipid peroxidation, which provokes ferroptosis [ [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] ]. Besides directly targeting GPX4, its expression was shown to be regulated by HSPA5, a target gene of ATF4.…”