Micropropagation of Tunisian Cultivars Olive Trees: Preliminary Results

Chaari-Rkhis, A.; Trigui, A.; Drira, Noureddine

doi:10.17660/actahortic.1999.474.12

Cited by 13 publications

(4 citation statements)

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“…As reported in earlier studies, in vitro multiplication efficiency in Olea europea species is widely dependent on culture medium, cytokinin type and its concentration [7]. Also, it seems that different olive tree cultivars require different basic medium formulations [38].…”

Section: Effect Of Types and Concentrations Of Cytokinins On Shoot Gr...mentioning

confidence: 99%

Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar

et al. 2021

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Micro-propagation of olive provides the possibility of mass production true to type plants with appropriate attributes in short period of time, compared to conventional methods. Micropropagation of olive requires optimization of protocols adapted to specific cultivars requirements. This experiment was carried out during 2020 and 2021 seasons in tissue culture laboratory of Pomology Department, Faculty of Agriculture, Cairo University in order to evaluate the potential effects of Nano silver at 0, 5, 10 and 20 mg L -1 to improve sprouting percentage and shoot growth of Picual olive cv. The effect of nutrient media composition (MS modified, OM and OM modified) and cytokinins (BAP and Zeatin) on multiplication rate and shoot growth were examined. IBA and NAA (2 and 4 mg L -1 ) were examined during rooting stage. The obtained results cleared that silver Nanoparticles (AgNPs) at 5 mg L -1 recorded the highest percentage of bud sprouting, shoot length, number of shoots/explant and number of leaves/ shoot. Olive medium (OM) recorded the highest shoot length, number of shoots/explant and number of leaves/ shoot compared with OM modified and MS modified media. Also, Zeatin at 4 mg L -1 recorded the highest shoot growth parameters during in vitro multiplication stage. During in vitro rooting stage, both the type and concentration of auxins have a significant influence on number of roots, root length and rooting % of Picual olive cultivar. In conclusion, it is recommended that very low concentrations of Nano silver particles to be used in plants tissue culture on olive media (OM) supplemented with Zeatin at 4mg L -1 .

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Section: Effect Of Types and Concentrations Of Cytokinins On Shoot Gr...mentioning

confidence: 99%

Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar

et al. 2021

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“…The type of rooting substrate can greatly affect the success of root formation as a result of specific properties such as substrate porosity, water retention capacity, pH and level of nutrients (Therios, 2009). In the case of microcuttings, culture media composition is detrimental to the efficiency of in vitro multiplication, as mineral composition of the medium (Cozza et al, 1997;Rugini and Pannelli, 1993), growth regulators (Chaari-Rkhis et al, 1997) and carbon source (Garcia et al, 2002;Leva et al, 1994) can determine the success of a micropropagation protocol. Application of growth retardants in combination with auxin can improve rooting ability, (Davis et al, 1985; possibly through inhibition of gibberellin biosynthesis (Rademacher et al, 1984).…”

Section: Other Factors Known To Affect Adventitious Rootingmentioning

confidence: 99%

Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation

Porfírio

Silva

Cabrita

et al. 2016

Scientia Horticulturae

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Olive (Olea europaea) is one of the most important fruit species in the Mediterranean basin, where 95% of the world's olive orchards are planted, and it has become an economically valuable crop worldwide, due to an increasing interest in olive oil for human consumption. New olive orchards are being planted outside the Mediterranean, calling for an effort to identify the genotypes best adapted to the new conditions. However, some olive cultivars remain difficult to propagate, which significantly reduces the capacity to use the full genetic diversity of the species. Improving rooting ability in cuttings from recalcitrant olive cultivars has become a critical topic, which implies fundamental research on the anatomy, physiology, biochemistry and genetics of the adventitious root formation process. Besides, the existence of different rooting behaviors among olive cultivars also makes the species a candidate model plant for these studies. Olive propagation techniques evolved through time from field-or nursery-planted hardwood cuttings, to semi-hardwood cuttings in greenhouses under mist, and, more recently, to in vitro culture techniques. Nevertheless, research about adventitious root formation carried on each propagation method was mostly based on trial and error approaches. Researchers have mainly investigated different factors involved in the process of adventitious rooting by testing their effect in the rooting capacity of different cultivars, leading to a high dispersion and fragmentation of the available information. The goal of this review is to present the most relevant results achieved on adventitious root formation in olive cuttings, aiming to provide an integrated perspective of the current knowledge.

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“…It is a recognized fact that establishment of an optimized in vitro micropropagation protocol for woody plants has always remained an aspect of potential concern (Fabbri et al 2009) (Zuccherelli and Zuccherelli, 2002;Chaari-Rkhis et al, 2003;Micheli et al, 2009;Chaari-Rkhis et al, 2011). However, the novelty of the current study is supplementation of this hormone in olive media at higher concentration which showed comparatively better results as supported by Chaari -Rkhis et al (2011).…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of shoot induction and proliferation protocol for olive cultivars by assessing morpho-physiologic effects of pre-cooling and growth regulators

2017

Int. J. Biosci.

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To set a definite micro-propagation protocol for olive has always remained a challenging task owe to its woody nature. This study was consist of different olive cultivars (Arbosana, Arbequina, Koroneiki and Sorany) to devise a comprehensive shoot induction and proliferation protocol of olive media supplemented with various combinations of growth regulators (zeatin and BAP) and pre-cooling treatments (0, 24 and 48 hours). The in vitro performance of all the cultivars was assessed using a range of parameters including, morphological (length of primary shoot, percentage of induced shoots, number of leaves shoot-1 , number of shoots explant-1), and physiological (chlorophyll a, chlorophyll b, carotenoids, CO2 absorption. All cultivars indicated encouraging results for pre-cooling, and increasing concentration of hormones, zeatin and BAP. However, performance of 48 hrs pre-cooling, Arbosana and Arbequina cultivars, and zeatin hormone was outstanding. Furthermore, the interaction of 48 hrs pre-cooling with 2.5 mgL-1 zeatin exceed to BAP as revealed by the parameters included in the study. Likewise, SEM micrographs endorsed the higher affectivity of zeatin compared to BAP in accelerating the uptake of nutrients from olive medium. Findings of the study present more reliable results for olive shoot induction and proliferation as it basis on wide range of parameters.

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Micropropagation of Tunisian Cultivars Olive Trees: Preliminary Results

Cited by 13 publications

References 0 publications

Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar

Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar

Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation

In vitro evaluation of shoot induction and proliferation protocol for olive cultivars by assessing morpho-physiologic effects of pre-cooling and growth regulators

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