Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar

Hegazi, El Said Sadek; Yousef, Aml R.M.; allatif, A. M. Abd; Mahmoud, Thanaa Shaban Mohamed; Mostafa, Mona Kamal Mohamed

doi:10.21608/ejchem.2021.78774.3853

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…; while the higher concentration (20 and 30mg l − 1 ) recorded a lower leaf number per shoot of Dolce cv. Our results confirm that AgNPs can lead to an improvement in plant growth, which is consistent with our previously published data [ 42 , 43 ]. The obtained results indicated that AgNPs had a stimulatory effect on shoot regeneration of in vitro growing olive shoots of both cultivars and the impact of nanoparticles on shoot growth was highly dependent on NP concentrations [ 44 , 45 ].…”

Section: Resultssupporting

confidence: 93%

Unveiling the silver lining: examining the effects of biogenic silver nanoparticles on the growth dynamics of in vitro olive shoots

Hasanin,

Hassan,

AbdAllatif

et al. 2024

Microb Cell Fact

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The current study aimed to evaluate the effects of biogenic silver nanoparticles (AgNPs) on growth behavior and leaf anatomy of in vitro growing shoots of ‘Picual’ and ‘Dolce’ olive cultivars. Biosynthesis of AgNPs was carried out using the cell-free filtrate of Fusarium oxysporum. The dimension and shape of the synthesized AgNPs have been analyzed using spectroscopy and topography analysis tools, confirming that the biosynthesis of AgNPs is a crystalline nanostructure with an average particle size of 37 nm. The shoots of the selected olive cultivars were cultured on Rugini olive medium-supplemented AgNPs at 0, 10, 20, and 30mg L− 1. The effect of genotypes on shoot multiplication was significant, ‘Picual’ recorded higher values of shoot growth parameters compared with ‘Dolce’ cultivar. Adding AgNPs to the culture medium significantly affected the growth of in vitro olive shoots. AgNPs at 20 and 30mg L− 1 produced higher values of the number of shoots, shoot length, and leaf number of Picual cv. compared with the control treatments, but the higher AgNPs concentration harmed the growth parameters of Dolce cv. and recorded lower growth values compared with the lower concentration (10mg L− 1). AgNPs had a significant effect on leaf morphology and their anatomical structure. The current results showed that the stimulatory effect of AgNPs on shoot growth of in vitro olive shoots is highly dependent on plant genotype and nanoparticle concentration.

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Section: Resultssupporting

confidence: 93%

Unveiling the silver lining: examining the effects of biogenic silver nanoparticles on the growth dynamics of in vitro olive shoots

Hasanin,

Hassan,

AbdAllatif

et al. 2024

Microb Cell Fact

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“…[103] and O. europaea cv. Picual [104]. Three NPs, i.e., AgNPs, selenium (SeNPs), and chitosan (CSNPs), were utilized in different cultivars of O. europaea (Manzanillo, Picual, and Koroneiki), and AgNPs showed positive effect on shoot growth, whereas CSNPs and control media had less effectiveness and SeNPs exerted a negative effect [36].…”

Section: Metal Npsmentioning

confidence: 99%

Multifaceted Role of Nanomaterials in Modulating In Vitro Seed Germination, Plant Morphogenesis, Metabolism and Genetic Engineering

Pathak,

Haq,

Meena

et al. 2023

Plants

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The agricultural practices of breeding, farm management and cultivation have improved production, to a great extent, in order to meet the food demands of a growing population. However, the newer challenges of climate change, global warming, and nutritional quality improvement will have to be addressed under a new scenario. Plant biotechnology has emerged as a reliable tool for enhancing crop yields by protecting plants against insect pests and metabolic engineering through the addition of new genes and, to some extent, nutritional quality improvement. Plant tissue culture techniques have provided ways for the accelerated clonal multiplication of selected varieties with the enhanced production of value-added plant products to increase modern agriculture. The in vitro propagation method has appeared as a pre-eminent approach for the escalated production of healthy plants in relatively shorter durations, also circumventing seasonal effects. However, there are various kinds of factors that directly or indirectly affect the efficiency of in vitro regeneration like the concentration and combination of growth regulators, variety/genotype of the mother plant, explant type, age of seedlings and other nutritional factors, and elicitors. Nanotechnology as one of the latest and most advanced approaches in the material sciences, and can be considered to be very promising for the improvement of crop production. Nanomaterials have various kinds of properties because of their small size, such as an enhanced contact surface area, increased reactivity, stability, chemical composition, etc., which can be employed in plant sciences to alter the potential and performance of plants to improve tissue culture practices. Implementing nanomaterials with in vitro production procedures has been demonstrated to increase the shoot multiplication potential, stress adaptation and yield of plant-based products. However, nanotoxicity and biosafety issues are limitations, but there is evidence that implies the promotion and further exploration of nanoparticles in agriculture production. The incorporation of properly designed nanoparticles with tissue culture programs in a controlled manner can be assumed as a new pathway for sustainable agriculture development. The present review enlists different studies in which treatment with various nanoparticles influenced the growth and biochemical responses of seed germination, as well as the in vitro morphogenesis of many crop species. In addition, many studies suggest that nanoparticles can be useful as elicitors for elevating levels of important secondary metabolites in in vitro cultures. Recent advancements in this field also depict the suitability of nanoparticles as a promising carrier for gene transfer, which show better efficiency than traditional Agrobacterium-mediated delivery. This review comprehensively highlights different in vitro studies that will aid in identifying research gaps and provide future directions for unexplored areas of research in important crop species.

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“…Many studies stressed the positive effect of nanomaterials foliar application [30]. Attractive strategies to deal with the PVY problem include the development of new antiviral nanoparticles and using tissue culture techniques to facilitate the propagation of virus-free materials to limit viral transmission [31]. Both approaches of antiviral nanoparticles and tissue culture technique are essential and eventually a combination of the two techniques will be the most effective strategy in controlling the PVY epidemic by diminishing the incidence of plant-to-plant transmission events [32].…”

Section: Introductionmentioning

confidence: 99%

Effect of Chitosan Nanoparticles (CS-NPs) on In Vitro Regeneration Response and Production of Potato virus Y (PVY)-Free Plants of Potato

et al. 2022

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Potato virus Y (PVY) causes serious loss in the yield and quality of potatoes. The effect of chitosan nanoparticles (CS-NPs) on the regeneration response and production of PVY-free plants under in vitro conditions was studied. Murashige and Skoog (MS) medium supplemented with 0.1 mg L−1 Gibberellic acid (GA3), 0.1 mg L−1 Naphthalene acetic acid (NAA) and 500 mg L−1 malt extract was used for regeneration of plantlets from sprouts. Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) and Reverse Transcript Polymerase Chain Reaction (RT-PCR) was used for virus indexing of the mother plant and in vitro-regenerated plantlets. Explants of PVY positive potato plants were cultured on same medium amended with 100, 200, 250, and 300 mg L−1 of (CS-NPs). Shoot regeneration decreased from 100 to 200 mg L−1 as the concentrations of antiviral (CS-NPs) up to 250 mg L−1. It was decreased with the increase in the concentration of the antiviral (CS-NPs) up to 300 mg L−1. Antiviral (CS-NPs) at the concentration of 250 mg L−1 showed a positive effect on shoot regeneration. In vitro-regenerated plantlets were virus free and tested negative in both ELISA and RT-PCR. The level of 100 mg L−1 of (CS_NPs) produced 38.8% PVY-free plants and 30.50% of cultures showed shoot regeneration. The level of 200 mg L−1 of (CS-NPs) produced 49.6% PVY-free plants while 46.8% of cultures showed shoot regeneration. The level of 250 mg L−1 of (CS-NPs) was the most effective and produced 100.0% PVY-free plants and 100.0% of cultures showed shoot regeneration. Histopathological changes simultaneously with elucidation of resistance and growth enhancement were evident in the treated plants with (CS-NPs) than those untreated control. In conclusion, (CS-NPs) treatment is an effective tool to produce PVY-free sprouts explants and has potential for producing virus-free planting material for the potato industry.

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Effect of Silver Nanoparticles, Medium Composition and Growth Regulators on in vitro propagation of Picual Olive Cultivar

Cited by 5 publications

References 33 publications

Unveiling the silver lining: examining the effects of biogenic silver nanoparticles on the growth dynamics of in vitro olive shoots

Unveiling the silver lining: examining the effects of biogenic silver nanoparticles on the growth dynamics of in vitro olive shoots

Multifaceted Role of Nanomaterials in Modulating In Vitro Seed Germination, Plant Morphogenesis, Metabolism and Genetic Engineering

Effect of Chitosan Nanoparticles (CS-NPs) on In Vitro Regeneration Response and Production of Potato virus Y (PVY)-Free Plants of Potato

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