In vitro evaluation of shoot induction and proliferation protocol for olive cultivars by assessing morpho-physiologic effects of pre-cooling and growth regulators

Abstract

To set a definite micro-propagation protocol for olive has always remained a challenging task owe to its woody nature. This study was consist of different olive cultivars (Arbosana, Arbequina, Koroneiki and Sorany) to devise a comprehensive shoot induction and proliferation protocol of olive media supplemented with various combinations of growth regulators (zeatin and BAP) and pre-cooling treatments (0, 24 and 48 hours). The in vitro performance of all the cultivars was assessed using a range of parameters including, morphological (length of primary shoot, percentage of induced shoots, number of leaves shoot-1 , number of shoots explant-1), and physiological (chlorophyll a, chlorophyll b, carotenoids, CO2 absorption. All cultivars indicated encouraging results for pre-cooling, and increasing concentration of hormones, zeatin and BAP. However, performance of 48 hrs pre-cooling, Arbosana and Arbequina cultivars, and zeatin hormone was outstanding. Furthermore, the interaction of 48 hrs pre-cooling with 2.5 mgL-1 zeatin exceed to BAP as revealed by the parameters included in the study. Likewise, SEM micrographs endorsed the higher affectivity of zeatin compared to BAP in accelerating the uptake of nutrients from olive medium. Findings of the study present more reliable results for olive shoot induction and proliferation as it basis on wide range of parameters.

An efficient method for callus induction and shoot regeneration of Olea europea L. cv. koroneiki

“…It was also reported that each cultivar responds varyingly to plant growth regulators [36]. Shoot regeneration via nodal explant in olive has been generally accomplished through zeatin, BAP, TDZ and GA 3 in previous studies [28,37].…”