Micropropagation of Teucrium fruticans L., an ornamental and medicinal plant

Frabetti, M.; Gutiérrez-Pesce, P.; Gyves, Emilio Mendoza-de; Rugini, Eddo

doi:10.1007/s11627-009-9192-z

Cited by 22 publications

(21 citation statements)

References 20 publications

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“…In agreement, Prathanturarug et al (2012) found that IBA was superior to NAA in terms of root induction percentage and number of roots per shoot with S. hutanguriana regenerated shoots after 8 weeks in culture. A similar trend was also observed for other species including Centella asiatica (Tiwari et al 2000), Boscia senegalensis (Khalafalla et al 2011), Pappea capensis (Mngomba et al 2007) and Teucrium fruticans (Frabetti et al 2009). …”

Section: Discussionsupporting

confidence: 79%

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb

Bahar

Lahham

et al. 2012

Physiol Mol Biol Plants

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Moringa peregrina is an endangered species of Moringaceae. M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100 % shoot proliferation rate was observed on MS medium supplemented with 1.0 mg/l BA. On the other hand, MS medium supplemented with 1 mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation.

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Section: Discussionsupporting

confidence: 79%

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb

Bahar

Lahham

et al. 2012

Physiol Mol Biol Plants

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“…The rooting treatments consisted of full-and halfstrength MS medium supplemented with 0.0 to 24.6μM IBA (Table 3). In previous reports also, IBA proved to be the best for root regeneration of M. dioica (Hoque et al 2007;Hoque et al 1995;Nabi et al 2002;Karim and Ahmed 2010) and had marked effect on rooting of many species such as Centella asiatica (Tiwari et al 2000;Banerjee et al 1999), and Teucrium fruticans L. (Frabetti et al 2009). MS medium strength had significant impact on frequency of root induction, number of roots, and root length.…”

Section: Root Induction and Acclimatizationmentioning

confidence: 88%

In vitro propagation of spine gourd (Momordica dioica Roxb.) and assessment of genetic fidelity of micropropagated plants using RAPD analysis

Kumar

Singh

Prakash

et al. 2012

Physiol Mol Biol Plants

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An efficient protocol for rapid in vitro clonal propagation of spine gourd (Momordica dioica Roxb.) genotype RSR/DR15 (female) and DR/NKB-28 (male) was developed through enhanced axillary shoot proliferation from nodal segments. Maximum shoot proliferation of 6.2 shoots per explant with 100 % shoot regeneration frequency was obtained from the female genotype on Murashige and Skoog's (1962) medium supplemented with 0.9μM N6-benzyladenine (BA) and 200 mg l -1 casein hydrolysate (CH). While from the male genotype the optimum shoot regeneration frequency (86.6 %) and 6.4 shoots per explant was obtained on MS medium supplemented with 2.2μM BA. CH induced vigorous shoots, promoted callus formation, and proved inhibitory for shoot differentiation and shoot length, especially in explants from male genotype. Rooting was optimum on half-strength MS medium (male 92.8 %, female 74.6 %) containing 4.9μM indole-3-butyric acid (IBA). Plantlets were transferred to plastic cups containing a mixture of cocopit and perlite (1:1 ratio) and then to soil after 2-3 weeks. 84 % female and 81 % male regenerated plantlets survived and grew vigorously in the field. Genetic stability of the regenerated plants was assessed using random amplified polymorphic DNA (RAPD). The amplification products were monomorphic in the in vitro propagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic integrity of in vitro propagated plants. This micropropagation procedure could be useful for raising genetically uniform planting material of known sex for commercial cultivation or build-up of plant material of a specific sex-type.

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“…Nodal segments are preferred explants for micropropa- gation due to presence of preexisting meristems, which can be developed easily into shoots while maintaining clonal fidelity (Frabetti et al 2009). Season of explants collection markedly influences the establishment, growth and regeneration characteristics of in vitro cul- tures.…”

Section: Discussionmentioning

confidence: 99%

Assessment of factors on shoot proliferation potential of nodal explants of Phyllanthus fraternus and assessment of genetic fidelity of micropropagated plants using RAPD marker

et al. 2014

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Phyllanthus fraternus is an important medicinal plant, popularly known for its hepatoprotective and antiviral activities since ancient times. Various physiological factors like carbon sources, concentration of agar, pH of the media and effect of season of explants collection were optimized for high frequency regeneration of P. fraternus. The frequency of regeneration, average number and length of shoots were highly influenced by the type and concentration of carbon sources (monosaccharides and disaccharides, 1 to 4%), agar concentration (0.2 to 1%) and pH (4.5 to 6.8) of the media. Media containing 3% sucrose, 0.6% agar at pH 5.8 was best for regeneration. Seasonal variation of explants collection significantly affected the axillary shoots proliferation from explants and best proliferation was observed from explants collected during April to June. Genetic fidelity of regenerated plants was assessed by random amplified polymorphic DNA markers. No polymorphism was detected in micropropagated plants and the mother plant, revealing the genetic homogeneity of the in vitro raised plantlets. This is the first report regarding establishment of genetic fidelity of micropropagated P. fraternus plants, which could be successfully applied for the mass multiplication, germplasm conservation and further genetic transformation assays to meet the ever increasing demand of this medicinally potent plant for industrial and pharmaceutical uses.

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Micropropagation of Teucrium fruticans L., an ornamental and medicinal plant

Cited by 22 publications

References 20 publications

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

In vitro propagation of spine gourd (Momordica dioica Roxb.) and assessment of genetic fidelity of micropropagated plants using RAPD analysis

Assessment of factors on shoot proliferation potential of nodal explants of Phyllanthus fraternus and assessment of genetic fidelity of micropropagated plants using RAPD marker

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