Micropropagation of Strawberry Through Meristem Culture

Rattanpal, H. S.; Gill, M. I. S.; Sangwan, A.K.

doi:10.17660/actahortic.2011.890.19

Cited by 6 publications

(5 citation statements)

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“…Increasing the concentration and duration of exposure to surface sterilant decreased the survival rate of cultures, while increasing the number of aseptic cultures. This might be due to the phytotoxicity that HgCl 2 causes in plants (Wolella, 2017;Rattanpal et al, 2011). Optimum concentration and exposure time are essential for increasing the success rate of in vitro cultures because the inaccurate concentration will limit cell division and further growth of explants (Hashim et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Establishing surface sterilization protocol for clonal apple rootstock MM106

Lal,

Jamwal,

Bakshi

et al. 2023

JANS

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Surface sterilization is crucial in preparing viable and uncontaminated explants for tissue culture. Utilizing the right sterilizing agent in this procedure can efficiently eliminate the majority of surface contaminants. The present study aimed to establish the best sterilization procedure for clonal apple rootstock MM106 using shoot tips and nodal segments. Two important sterilizing agents, mercuric chloride (ranging from 0.05% to 0.3%) for various durations (2 to 6 minutes) and sodium hypochlorite (ranging from 3.0% to 12%) for different durations (10 to 30 minutes), were employed. The results indicated that the maximum aseptic conditions (80.00% and 80.67%) and survival rates of the cultures (60.66% and 67.33%) for shoot tips and nodal segments, respectively, were achieved through surface sterilization with 0.1% mercuric chloride for 4 minutes, specifically during April, in comparison to March and May of 2021 due to low phenol content and high meristematic activity. The results yielded valuable insights for efficiently propagating clonal apple rootstocks on a local scale, and they have the potential to offer guidance for establishing commercial facilities dedicated to producing these specific clonal apple rootstocks and varieties.

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Section: Discussionmentioning

confidence: 99%

Establishing surface sterilization protocol for clonal apple rootstock MM106

Lal,

Jamwal,

Bakshi

et al. 2023

JANS

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“…IBA is extensively utilized for the in vitro rooting of various strawberry cultivars, including cv. 'Ofra' (Anuradha et al 2016), 'Festival' (Sharma, Kumar 2012;Khalil et al 2019), 'Oso Grande' (Lal et al 2003;Rattanpal et al 2011), 'Chandler' (Lal et al 2003), 'CamaRosa' (Lal et al 2003;Haddadi et al 2010) and 'Sweet Charlie' (Sharma, Kumar 2012;Harugade et al 2014;Khalil et al 2019). Efficacy of IBA and activated charcoal for in vitro root formation was reported for cvs.…”

Section: Discussionmentioning

confidence: 99%

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2021

EEB

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The objective of this study was to develop a protocol for large-scale propagation of strawberry cv. 'Sweet Charlie' and 'Winter Dawn' using the micropropagation technique. Shoot cultures were obtained from shoot tips on Murashige and Skoog (MS) medium with 4% table sugar, 0.75% agar, 5 mg L −1 6-benzyladenine and 0.01 mg L −1 kinetin. These shoots were multiplied and maintained on MS medium with 1 mg L -1 6-benzyladenine and 0.1 mg L -1 kinetin. Rooting of in vitro raised shoots was successfully conducted by pulse treatment with 500 mg L -1 indole-3-butyric acid for 30 s and subsequent culturing in MS medium with 1 mg L -1 indole-3-butyric acid, 0.1 g L -1 activated charcoal and 6% table sugar. Plants were successfully acclimatized and survived in field conditions.

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“…This concentration resulted with higher survival percent in such varieties as: 'Chandler', 'Oso Grande', 'Toro' and 'Islamabad Local' [Munir et al 2015]. In Rattanpal et al [2011] studies for sterilization, plants was dipped in 0,1% solution of mercury (II) chloride (HgCl 2 ) for 4 min.…”

Section: Explant Selection and Sterilizationmentioning

confidence: 99%

“…'Camarosa' variety survival rate at about 90% was obtained on mixture of perlite, vermiculite and coconut fiber (2 : 1 : 2 v/v/v) [Haddadi et al 2010]. A relatively high plant survival rate was also obtained during 'Oso Grande' acclimatization on mixture of garden soil and manure in the ratio 1 : 1 (77.3%) [Rattanpal et al 2011].…”

Section: Acclimatizationmentioning

confidence: 99%

In vitro REGENERATION OF Fragraria PLANTS

Rokosa

Mikiciuk

2017

Acta Sci. Pol. Hortorum Cultus

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Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness -from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.

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Micropropagation of Strawberry Through Meristem Culture

Cited by 6 publications

References 0 publications

Establishing surface sterilization protocol for clonal apple rootstock MM106

Establishing surface sterilization protocol for clonal apple rootstock MM106

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In vitro REGENERATION OF Fragraria PLANTS

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