Micropropagation of Nerium Oleander through the Immature Pods

Abstract

Oleander (Nerium oleander L.) is a vegetatively propagated ornamental plant valued for its evergreen foliage and showy terminal flower clusters that are available in different colours. Oleander is cultivated recently as a flowering pot plant and therefore abundant propagation plant material for commercial use is of great importance. This species also produces secondary metabolites (Paper & Franz 1989), some of which are of pharmacological interest. In vitro culture of plants has gained importance during recent years because, besides other application, this technique can be used for the rapid multiplication of some plants (Tisserat 1987). As far as we are aware, there are no published reports about micropropagation of Nerium oleander and the aim of the present work was to determine the culture conditions for micropropagation of this plant.To induce callus formation from pods of Nerium oleander,and study the in vitro growth conditions of calli. Study the specific growth factor for root and shoot induction, and transplantation and acclimatization of explants and isolation of the DNA from explants. Standardization of micropropagation protocol of Nerium oleander.In the case of Nerium oleander, the micropropagation technique from pods were established. We also raised plantlets from the callus and also subjected the DNA for analysis and done RAPD to find the difference between the normal and in vitro plants, the result is no difference-this shows that the plants are identical. Standardization of surface sterilization protocol, aseptic culture initiation,establishment and multiplication, suitable media for rooting and a suitable protocol for hardening in order to achieve quality transplant.Optimizitation of medium for callus induction, maintenance and regeneration.

Standardization of zygotic embryo culture from Nerium oleander L. and comparative analysis of biosynthesized cardiac glycosides within in vitro and acclimatized plants

“…In the present study, the germination percentage of N. oleander zygotic embryos was found much higher than the wild mature seeds in the culture medium, which might be due to the presence of various inhibitors in the seed coat (Kaveri & Rao, 2015). However, some other direct (Karrunakaran & Shri, 2010;Hatzilazarou et al, 2019) and indirect in vitro micropropagation techniques are reported from this plant (DH Paper, 1989;Santos et al, 1994;Vila et al, 2010) yet no ZEC and CGs profiling of acclimatized and in vitro raised plants found to be reported in our knowledge. ZEC depends on many factors including the source of carbon and other nutrients used, which helps in the germination, survival and growth of the delicate IVP.…”

Exploitation of Plant Extracts (Stem and Root) of Micropropagated Bacopa Monnieri : Antimycotic Potential

“…In Asian country and therefore the tropics it grows naturally in wet soil, shallow water and marches. The herb can be found at elevations from sea level to altitudes of 4,400 feet and is easily cultivated if adequate water available (Soundararajan et al, 2011). It has a great market demand due to its high medicinal uses.…”

Assessment for the Incidence of Number of Subcultures on Genotype Stability for In Vitro Plantlets of Yam (Dioscorea spp.) Using RAPD Markers

“…Somaclonal variation is defined as genetic and phenotypic variation among clonally propagated plants of a single donor clone (Larkin, 1987). According to recent reports, somaclonal variations were detected among micropropagated plantlets in many taxa at levels such as phenotypic and cytological (Podwyszyńska, 2005;Zayova et al, 2010), biochemical and molecular (Soundararajan and Karrunakaran, 2010;Kanungo et al, 2012;Senapati et al, 2012;Saha et al, 2012).…”