Objective: The prevalence of invasive mycoses is increased in the immunocompromised patients with an increase in resistance developed against current antifungal drugs. This has led to the need for discovering novel combinations of the antifungal drugs to combat against resistant pathogenic spp. This study mainly targets to evaluate the antifungal activity of ibuprofen (IBU) alone and in combination with the standard antifungal drugs (polyenes and azoles) against eight isolates of Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger.
Methods:The study was performed using the disc diffusion assay (DDA), microbroth dilution assay and spore germination inhibition assay. Moreover, cytotoxicity was checked by heamolytic assay.Results: Minimum inhibitory concentration (MIC) of IBU against A. fumigatus and A. flavus using DDA is found to be in the range of 250-275 µg/disc while for A. niger isolates, the range was 500-575 µg/disc. Likewise, by broth microdilution assay and spore germination inhibitory assay, MIC determined, were in the range of 500-750 µg/ml against A. fumigatus and A. flavus while for A. niger, it was 1000-1500 μg/ml.
Conclusion:IBU demonstrated its antimycotic potential against all the eight isolates of Aspergillus spp. Moreover, preliminary combinatorial evaluation of IBU with the standard antifungal drugs reported by DDA revealed an increase in zone of inhibition as compared to the drugs alone. Further research regarding the confirmation of synergistic interaction between the selected drugs is in progress.
This exploratory research paper is on antimicrobial compound. That life form was named as NK2. It was observed to be hostile to both bacterial and parasitic test creatures. Generation of anti-infection was more in a recently defined soup. Anti-toxin creation achieved a most extreme toward the finish of the 70 h of aging by blending cup culture. The antimicrobial compound was extricated in n-butanol, ethyl acetic acid derivation and methanol. Antimicrobial compound, which was created by the dirt confine NK2 did not demonstrated cytotoxic action on Vero cell lines.
The goal of this review was to disconnect and describe a rhizospheric bacterium from Sorgastrum nutans, developing around the betray district of Rajasthan (India). Plant growth promoting rhizobacterias (PGPR) are known to impact plant development by different immediate or circuitous systems. Separated strain was tried for different PGP characteristics like 1-aminocyclopropane-1-carboxylate (ACC) deaminase movement, phosphate solubilization, indole acidic corrosive generation, creation of siderophore, nitrogen obsession and alkali generation. Bio-control capacity of detach was screened by antagonistic action against certain parasitic/bacterial pathogens and additionally Hydrogen cyanide( HCN) creation. Selected test strain was likewise biochemically characterized. Advance recognizable proof of identification was performed by PCR based 16S rRNA qualitative sequencing. Besides assessment of the segregate SNP-18 showing different plant growth promoting (PGP) qualities on soil/plant framework is on-going to reveal their viability as successful PGPR.
Bacopa monnieri is commonly called as brahmi or jal brahmi in India. Brahmi is a non-aromatic herb Brahmi is considered as the herb played a very important role in Ayurvedic medicine. It is found easily India, Australia, Europe, Africa, Asia. Bacopa monnieri (L) belongs to the family Scrophulariaceae is an amphibious plant of tropical and normally found growing on the banks of the rivers and lakes. It is commonly called as brahmi or jal brahmi in India. Brahmi is considered as the main rejuvenating herb played a very important role in Ayurvedic therapies. It also has anti-inflammatory, analgesic, antipyretic, epilepsy, anticancer, antioxidant activities and recently antimycotic preoperty has been reported. The micro propagation protocol of the medicinally important plant Bacopa monnieri was standardized using nodal segments as explants. They were surface sterilized with HgCl2 (0.1%) for 3 minutes prior to inoculation on MS media supplemented with BAP (0.5- 2.5 mg/l); IAA (0.1-0.5 mg/l for shooting,1.0-1.5 mg/l for rooting); NAA (0.1- 0.5 mg/l for shooting, 1.0-1.5 mg/l for rooting). The best performance for shoot multiplication was showed in MS medium supplemented with 1.5 mg/l BAP + 0.5 mg/l IAA. In this combination the number of shoots per explant was 16 and average length of shoot 5.54 ± 0.54 cm. But when different concentrations of NAA were applied along with 1.5 mg/l BAP the number of shoots per explant was 14 and average shoot length was 3.46 ± 0.43 on media. For root induction, best rooting was observed with half strength of MS medium supplemented with IAA (1.0 mg/l). In this combination, it was observed that the number of roots was 12 and average root length of 2.80 ± 0.09. The present study is a stepping stone for in vitro production of required active principles of Bacopa monnieri.
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