Background:The anticancer and antioxidant effects of the aqueous extract of Indigofera aspalathoides on 20-methylcholanthrene (20-MCA) induced fibrosarcoma were investigated in male albino rats.Materials and Methods:The rats were divided into four different groups, each group consisting of six animals. Group I animals were served as normal control, Group II animals were fibrosarcoma-bearing animals after the incubation period, Group III animals were fibrosarcoma-bearing animals, treated with aqueous extract of I. aspalathoides intraperitoneally at a dose of 250 mg/kg b.w. for 30 days and Group IV animals were administered with the aqueous extract of I. aspalathoides alone, at a dose of 250 mg/kg b.w. for 30 days, served as drug control animals. After the experimental period, all the rats were weighed and killed by cervical decapitation. The serum was separated from the blood for analysis. The weights of the liver and the kidneys were noted. The fibrosarcoma was proved by pathological examinations. The liver and kidney tissues were excised and then homogenized in an ice-cold buffer. These tissues were used for biochemical analysis.Results:The activities of antioxidant enzymes, e.g. catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), in blood serum, liver, and kidney of control and experimental animals, respectively, have been reported.Conclusion: The present observations suggested that the aqueous extract of I. aspalathoides treatment enhanced the recovery from 20-MCA-induced fibrosarcoma due to its antioxidants and antineoplastic properties.
Oleander (Nerium oleander L.) is a vegetatively propagated ornamental plant valued for its evergreen foliage and showy terminal flower clusters that are available in different colours. Oleander is cultivated recently as a flowering pot plant and therefore abundant propagation plant material for commercial use is of great importance. This species also produces secondary metabolites (Paper & Franz 1989), some of which are of pharmacological interest. In vitro culture of plants has gained importance during recent years because, besides other application, this technique can be used for the rapid multiplication of some plants (Tisserat 1987). As far as we are aware, there are no published reports about micropropagation of Nerium oleander and the aim of the present work was to determine the culture conditions for micropropagation of this plant.To induce callus formation from pods of Nerium oleander,and study the in vitro growth conditions of calli. Study the specific growth factor for root and shoot induction, and transplantation and acclimatization of explants and isolation of the DNA from explants. Standardization of micropropagation protocol of Nerium oleander.In the case of Nerium oleander, the micropropagation technique from pods were established. We also raised plantlets from the callus and also subjected the DNA for analysis and done RAPD to find the difference between the normal and in vitro plants, the result is no difference-this shows that the plants are identical. Standardization of surface sterilization protocol, aseptic culture initiation,establishment and multiplication, suitable media for rooting and a suitable protocol for hardening in order to achieve quality transplant.Optimizitation of medium for callus induction, maintenance and regeneration.
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