Micropropagation of <i>Eclipta alba</i> (Linn.) Hassk- a Valuable Medicinal Herb

Abstract

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl -1 BAP + 0.1 mgl -1 NAA, in which 94% of the explants produced 18 shoots per culture.Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl -1 IBA +1.0 mgl -1 NAA.For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks.The survival rate of regenerated plantlets was 80%.

Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

“…Combination of BAP with IAA was not found suitable for multiple shoot induction. This is identical with the results obtained by Hassan et al, 2008 using apical and axillary bud. The maximum number (9.50±0.70) of shoot induction was found in MS +1.0 mg/l BAP + 0.1 mg/l IAA.…”

“…The results of these experiments are presented in Table 3. Use of auxins singly or in combination for rooting in E. alba were also reported by different authors (Hassan et al, 2008, Baskaran & Jayabalan, 2003, Sharma et al, 2013. Among the different auxins (IBA, IAA and NAA) the best root induction and development was found in half strength MS medium containing 0.1 mg/l IBA.…”

Mass Scale Micropropagation Of Andrographis Paniculata (Burm. F.) Wall Ex Nees

“…The highest number of roots per shoot and mean length of roots was noted in half strength MS medium fortified with 2.0 mg/l IAA (Table 4). Such influence of IAA for induction and proliferation of root growth has been reported in other medicinal plants including Vitex negundo (Chandramu et al 2003, Jawahar et al 2007, Abrus precatorius Biswas et al 2007), Eclipta alba (Hassan et al 2008) The in vitro grown complete plantlets were finally transferred from the culture room to keep outside pots containing garden soil and compost of ratio of 1:1 through successive phases of acclimatization (Fig. 1D).…”

“…in A. paniculata and in many other medicinal plants including Curculigo orchioides(Wala and Jasrai 2003), Paedaria foetida(Amin et al 2003), Withania somnifera(Supe et al 2006), Eclipta alba(Hassan et al 2008), Boerhaavia diffusa(Roy 2008, Biswas et al 2009), Ficus religiosa (Hasan et al 2009. Rauvolfia serpentina(Singh et al 2009), Plumbago indica (Bhadra et al 2004, Wedilia chinensis(Sultana and Handique 2004).…”

An Efficient Micropropagation Protocol For Mucuna Pruriens (L.) Dc.

“…The maximum number of multiple shoot buds was produced on MS medium supplemented with 1.5 mg/l BAP + 1.0 mg/l IAA. Such direct organogenesis was also reported in many other medicinal plants including, Paedaria foetida (Amin et al 2003), Rouvolfia tetraphylla (Faisal et al 2005), Withania somnifera (Supe et al 2006), Eclipta alba (Hassan et al 2008), Boerhaavia diffusa (Roy 2008, Biswas et al 2009, Ficus religiosa (Hasan et al 2009), Rauvolfia serpentina (Singh et al 2009). On the other hand, leaf segments always produced green friable callus tissue (Fig.…”