Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

Yesmin, Sabina; Hashem, Abu; Islam, Soriful

doi:10.3329/jujbs.v4i1.27786

Cited by 4 publications

(3 citation statements)

References 18 publications

(22 reference statements)

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“…Similar results were also obtained in cucumber ( Abu-Romman et al., 2015 ). On the other hand, the effectiveness of BA over KN for shoot regeneration has been earlier documented in Eclipta alba ( Yesmin et al., 2016 ) and Gymnema sylvestre ( Isah, 2019 ). The effective role of adenine sulfate at a higher concentration (25 mg/L) in the culture medium improves the frequency of shoot development and helps in the recovery of the leaves from chlorotic symptom in W. chinensis ( Tsai and To, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Enhanced wedelolactone content in in vitro-raised genetically uniform Wedelia chinensis under the influence of CuSO4

Swami,

Nimker,

Narula

et al. 2023

Front. Plant Sci.

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In the present study, we addressed the imperative for potent anticancer agents through Wedelia chinensis, a medicinal plant abundant in the robust antihepatotoxic and antitumor compound wedelolactone. Hindrances in conventional propagation methods due to cross-pollination and habitat degradation prompted us to pioneer in vitro rapid multiplication using plant tissue culture. Optimal outcomes were attained employing Murashige and Skoog (MS) medium supplemented with Indole-3-butyric acid (IBA) (0.5 mg/L) and Kinetin (KN) (5.0 mg/L), yielding 97.67% shoot regeneration and 81.67% rooting from nodal explants. Transplanted plantlets exhibited a 92% survival rate. We established a wedelolactone extraction protocol using toluene:ethyl acetate:formic acid (5:4:1) for High-performance thin-layer chromatography (HPTLC) analysis, trailblazing wedelolactone quantification and 2C DNA analysis in W. chinensis via flow cytometry. Experiments under heavy metal stress with CuSO4 unveiled physiological responses, with peak wedelolactone content [193.90 μg/g dry weight (dw)] in vitro at 75 μM CuSO4, surpassing in vivo levels (89.95 μg/g dw) by 116%. By pioneering successful in vitro rapid multiplication and enhanced wedelolactone content, we bridge a critical gap in the conservation and production of this medicinal plant. Our findings not only offer a sustainable means of propagation but also present a viable strategy for elevating the yield of potent bioactive molecules like wedelolactone, holding immense promise for the development of novel therapeutic interventions and addressing the pressing healthcare challenges of our time.

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Section: Discussionmentioning

confidence: 99%

Enhanced wedelolactone content in in vitro-raised genetically uniform Wedelia chinensis under the influence of CuSO4

Swami,

Nimker,

Narula

et al. 2023

Front. Plant Sci.

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“…The problem can be overcome with the development of rapid, efficient, simple, and most importantly reproducible micropropagation protocol for the selected plants (Moraes et al 2021). A similar issue arose with E. alba as well, while there are reports on its in vitro multiplication, they may be genotypedependent and their techniques lack consistency (Dhaka and Kothari 2005;Singh et al 2010;Ragavendra et al 2014;Yesmin et al 2015). Thus arises the need for the development of a genotypespecific micropropagation protocol for E. alba to meet ever increasing demand of the industry.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Plant Growth Regulators on Micropropagation of Eclipta alba: A Plant with Diverse Medicinal Properties

et al. 2022

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Synergism between various plant growth regulators is reported to be a key factor for the development of efficient in vitro propagation for any plant. Therefore, the current study examines the synergistic impact of plant growth regulators on in vitro propagation of Eclipta alba, an important medicinal plant possessing diverse medicinal properties. For the establishment of aseptic cultures, nodal segments were employed as explants on MS medium supplemented with 2.5 μM of 6-benzyle adenine (BA). Varying concentrations of BA and Kinetin (KIN)(0.0-5.0 μM), either alone or in combination with α- naphthalene acetic acid (NAA @ 0.0-5.0 μM) and indole 3-acetic acid (IAA@ 0.0-5.0 μM), were found to be effective for promoting shoot proliferation. Compared to KIN, BA was found to promote shoot proliferation and elongation more effectively. Further, the addition of 0.5μM NAA in the MS medium supplemented with 2.5 μM of BA increased shoot multiplication and elongation frequency from 58 and 17 percent to 65 and 21 percent respectively. The rooting frequency was found to be maximum on 1/2 strength MS medium supplemented with 5.0 μM of indole 3-butyric acid (IBA), which was found to be a superior auxin for inducing roots as compared to the NAA and IAA. With a 75% survival rate, in vitro raised plantlets were effectively acclimatized first in a poly house and later under greenhouse conditions. Molecular analysis was carried out using RAPD markers, with results indicating that the micropropagated plants were genetically identical to the mother plant. The developed micropropagation protocol for E. alba can be used at the commercial level for the mass multiplication of plants.

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“…Shoots were cultured in vitro on MS medium with and without IBA, IAA, and NAA. Half-strength MS with 0.1mg/l IBA was found to be the best root induction medium [79], with 96% of shoots taking root in it. Plant nodal explants from in vitro-grown Eclipta alba L. plants developed yellowish white, friable calluses after 3 weeks of growth on Murashige Skoog (MS) media supplemented with 10.75 mM -napthaleneacetic acid (NAA) and 9.04 mM 2, 4-dichlorophenoxyacetic acid (DOCA) (2,4-D).…”

Section: Nodal Segments As Explantsmentioning

confidence: 94%

A Review on Micropropagational and Phytochemical Aspects of Some Medicinally Important Wetland Plants

Syamkumar¹,

Geethalakshmi²

2022

ujps

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Wetlands are places where the soil is always moist. Plants that are found in areas that are always wet are called wetland plants. Sphaeranthus indicus, Eclipta alba and Scoparia dulcis all are wetland plants. The background of this study is to understand the different types of phytochemicals present in these three wetland plants and micropropagation methods using different types of explants. The bioactive constituents contained in these three wetland plants and micropropagation methods using various types of explants were learned from the previous articles. Phytochemicals such as sesquiterpene lactones, eudesmanolides, and flavonoids have been isolated from the plant Sphaeranthus indicus. Eclipta alba has yielded a diverse set of chemical compounds, including coumestans, alkaloids, thiopenes, saponins, quinines, flavonoids, polyacetylenes, triterpenes and their glycosides. The main bioactive compounds contained in the plant Scoparia dulcis are flavonoids, polyphenols, tannins and terpenoids. Micropropagation using different types of explants has been done very successfully for these three wetland plants. Phytochemicals mainly contained in these plants are alkaloids, terpenoids, steroids, flavonoids, coumestan. Explants used for micropropagation are leaf, shoot tip, nodal segments, apical buds, axillary buds and seeds. The main conclusion of this study is that all these plants have many medicinal properties that are yet to be discovered. All of which should be exposed to the latest technologies available in the field. However, the extent to which plant tissue culture can be used in these plants is also a possibility that should be considered.

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Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

Cited by 4 publications

References 18 publications

Enhanced wedelolactone content in in vitro-raised genetically uniform Wedelia chinensis under the influence of CuSO4

Enhanced wedelolactone content in in vitro-raised genetically uniform Wedelia chinensis under the influence of CuSO4

Synergistic Effect of Plant Growth Regulators on Micropropagation of Eclipta alba: A Plant with Diverse Medicinal Properties

A Review on Micropropagational and Phytochemical Aspects of Some Medicinally Important Wetland Plants

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