Among the in vitro derived different explants such as cotyledonary leaf, hypocotyl, shoot tip and root of two local varieties, namely Singhnath and Kazla (BARI Begun-4) of eggplant (Solanum melongena L.) cotyledonary leaf was found to be the best for multiple shoot regeneration. High frequency direct organogenesis of shoots was achieved from cotyledonary leaf in MS supplemented with 1.0 mg/l BAP and 1.0 mg/l Kn. Anatomical studies using freezing microtome supported the formation of shoots through organogenesis. Proliferation and elongation of such shoots were obtained in hormone free MS. Moreover, the regenerated shoots produced healthy roots when they were cultured on MS without hormonal supplements. Following the formation of roots the in vitro raised plantlets were successfully established in soil. Viable seeds were obtained from the in vitro raised mature plants.
Understanding of crop water requirement is essential for irrigation scheduling and selection of cropping pattern in any particular area. A study was conducted to estimate irrigation requirement and made irrigation scheduling of T. Aman (wet season) and Boro (dry season irrigated) rice in the western region of Bangladesh using CROPWAT model. Historical climate data from three weather stations in the region along with soil and crop data were used as input to FAO Penman-Monteith method to estimate reference evapotranspiration (ET o ). Effective rainfall was calculated using USDA soil conservation method. The model estimated1408 mm annual ET o in the study area, of which the highest amounts of 175 mm was in April and the lowest (70 mm) in December. The average annual rainfall was 1592 mm of which 986 mm was effective for plant growth and development. The model estimated ET c of BRRI dhan49, which was 473 to 458 mm, depending on its transplanting dates from 15 July to 15 August. Rice transplanted on 15 July required no irrigation, whereas three supplemental irrigations amounting 279 mm were required for transplanting on 15 August. The CROPWAT model estimated seasonal irrigation water requirement of 1212 mm (12 spilt applications) for BRRIdhan28 transplanted on 15 January. This model has also a potentiality to make irrigation scheduling of other crops.
An experiment was conducted to determine the impact of Plantain (PL), Bio-Sel-E and commercial broiler diet on the growth performance, feed conversion ratio, performance index and carcass yield of broiler. A total of 160 day old broiler chicks (Cobb-500) were randomly distributed into four groups with four replication in each group and having 10 birds in each replication. Basal diet was used for group 1 (control), Basal diet was supplemented with PL (0.5% of DM basis) and Bio-Sel-E (5g/50 birds in drinking water) for group 2 and 3 respectively and for group 4 used commercial broiler diet (Quality Feed Ltd.). Total feed intake was similar among the groups. Feed conversion ratios of PL diet, Bio-Sel-E diet, and commercial broiler diet were comparable, and were lower (P<0.05) than the control diet.Significantly higher body weight gain and performance index were found in PL, Bio-Sel-E and commercial broiler diet groups than control diet. The dressing yield of PL diet and commercial broiler diet were comparable, and they were higher (P<0.05) than Control diet and Bio-Sel-E diet. It could be concluded that the Supplementation of 0.5% PL in the diet had positive effect on growth, feed intake, feed conversion ratio, performance index and carcass characteristics of broilers almost similar of Bio-Sel-E and commercial broiler diet.
Multiple shoots were obtained from both shoot tips and nodal segments cultured on MS fortified with different concentrations of BAP and Kn singly or in combination with low concentration of NAA. Maximum number of shoots (9.28 ± 0.61) were found on MS supplemented with 1.5 mg/l BAP and 05 mg/l NAA. In vitro regenerated shoots were separated and transferred onto half and full-strength MS supplemented with different concentration of IBA, IAA and NAA for root induction. Full strength MS containing 0.2 mg/l IBA was found to be best for root induction where 93.33% shoots were rooted. In vitro regenerated plants grew normally without showing any morphological variation and flowered after 40 days of transplantation.
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