Micropropagation of Eucalyptus grandis  ×  E. urophylla AEC 224 clone

Oliveira, Cassiana de; Degenhardt, Juliana; Bettencourt, Gisela Manuela de França; Amano, Érika; Franciscon, Luziane; Quoirin, Marguerite

doi:10.1007/s11676-016-0282-6

Cited by 14 publications

(4 citation statements)

References 27 publications

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“…Phenolic oxidation has been a problem associated with the micropropagation of woody species and has been reported in several studies (Brondani, 2011;Oliveira et al, 2015;Oliveira et al, 2017). These results may be related to internal environmental factors that affect explant development, where smaller flasks tend to exhibit reduced concentrations of carbon dioxide and high concentrations of ethylene, but may also be affected by irradiation, air temperature and relative humidity (Xiao et al, 2011).…”

Section: Discussionmentioning

confidence: 94%

LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

et al. 2018

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Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.

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Section: Discussionmentioning

confidence: 94%

LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

et al. 2018

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“…Thus modulating the concentration of PGRs may help improve shoot induction and elongation. Orthogonal design is an efficient method of studying the effects of multifactor and multilevel, which has been widely used for establishment of a successful tissue culture method for diverse plants like Eucalyptus grandis × E. urophylla (Oliveira et al, 2017). In this study, a L9 (3 4 ) orthogonal design was performed as listed in Table 1 with the aim to determine an optimal PGRs combination for shoot induction and growth (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis

Cai

Cheng

Zhang

2019

Not Bot Horti Agrobo

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Trichosanthes kirilowii Maxim is a perennial plant possessing great medicinal and edible value. In this study, an efficient rapid propagation system was developed for in vitro production of high-quality plantlets of T. kirilowii cv. ‘Yuelou-2’ via organogenesis. Shoots were established from the nodal stems of this plant by cultured on Murashige and Skoog (MS) basal medium containing different concentrations of naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and 6-benzyladenine (6-BA) according to a L9 (34) orthogonal array design. MS medium supplemented with 0.5 mg l-1 6-BA alone conferred significant enhancement of shoot induction and development by variance and statistical analysis of shoot induction frequency, shoot length, as well as lateral shoot number and total node number per explant. Rooting percentage, root morphological characteristics, plantlet survival rate, as well as plantlet growth performance in vitro and ex vitro were comprehensively assessed, and results showed that the addition of 1.0 mg l-1 NAA to 1/2 MS medium was the most responsive for root induction and production of high-quality plantlets from the regenerated shoots. The in vitro propagation system described here has the potential for large-scale and rapid production of in vitro plantlets of seed variety of T. kirilowii in a sustainable manner. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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“…However, SP medium [108,[110][111][112], WPM minerals [113], WPM [114], B5 medium [115][116][117], N7 medium [86,118], JADS medium [98], EDM [119], and MS medium with White vitamins [89] have also been used. Callus is often induced in darkness, although basal organogenesis on shoots can occur in eucalypt shoot cultures that are maintained under light [45,46,64,98,120].…”

Section: Organogenesismentioning

confidence: 99%

“…Half-strength MS medium with 1/10 KNO 3 and 2.5× MgSO 4 has been used for E. grandis shoots [104], and half-strength MS medium with full-strength vitamins, 2.66 µM riboflavin, and 0.93 µM β-carotene has been used recently for E. grandis × E. urophylla shoots [114]. MS medium has also been reduced to quarter-strength during root induction, including for E. grandis, E. grandis × E. nitens, and E. grandis × E. urophylla shoots [124,[151][152][153].…”

Section: Adventitious Root Formationmentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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Micropropagation of Eucalyptus grandis × E. urophylla AEC 224 clone

Cited by 14 publications

References 27 publications

LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis

Tissue Culture of Corymbia and Eucalyptus

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