Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis

Abstract

Trichosanthes kirilowii Maxim is a perennial plant possessing great medicinal and edible value. In this study, an efficient rapid propagation system was developed for in vitro production of high-quality plantlets of T. kirilowii cv. ‘Yuelou-2’ via organogenesis. Shoots were established from the nodal stems of this plant by cultured on Murashige and Skoog (MS) basal medium containing different concentrations of naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and 6-benzyladenine (6-BA) according to a L9 (34) orthogonal array design. MS medium supplemented with 0.5 mg l-1 6-BA alone conferred significant enhancement of shoot induction and development by variance and statistical analysis of shoot induction frequency, shoot length, as well as lateral shoot number and total node number per explant. Rooting percentage, root morphological characteristics, plantlet survival rate, as well as plantlet growth performance in vitro and ex vitro were comprehensively assessed, and results showed that the addition of 1.0 mg l-1 NAA to 1/2 MS medium was the most responsive for root induction and production of high-quality plantlets from the regenerated shoots. The in vitro propagation system described here has the potential for large-scale and rapid production of in vitro plantlets of seed variety of T. kirilowii in a sustainable manner.
 
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 In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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In vitro cluster buds regeneration and control of Shoot Tip Necrosis in tissue cultures of Trichosanthes cucumerina Linn.

“…Appropriate explant sources are essential for successful plant micropropagation system (Sokolov et al 2014). In the micropropagation of T. cucumerina, seeds (Hwang, 2007), shoot tips (Devendra et al 2008), stem segments (Joo et al 2019;Cai et al 2019), andleaves (Zhao et al 2018) are commonly used as explants. Among these, stem segments are frequently employed to induce cluster buds.…”

“…Secondly, asexual reproduction methods, such as ramet propagation and cutting propagation, are often employed in the actual breeding and production of T. cucumerina. These methods have a low proliferation coefficient and require consuming a large number of parent plants, which increases the production cost (Cai et al 2019;Hu et al 2020). Due to the limitations of the aforementioned methods, researchers need to find a more efficient and stable propagation method for T. cucumerina, such as plant tissue culture.…”

“…Plant tissue culture technology plays a crucial role in germplasm innovation, breeding, and germplasm resource preservation, serving as the primary and most effective method for industrial seedling production of T. cucumerina (Kyte et al 2013;Bridgen et al 2018;Dhumal et al 2020). Commonly used explants for in vitro culture of Trichosanthes include seeds (Hwang 2007), shoot tips (Devendra et al 2008), stem segments (Joo et al 2019;Cai et al 2019), and leaves (Zhao et al 2018); Xu et al (2015) even successfully achieved in vitro regeneration of T. cucumerina by inducing somatic embryos. However, most published studies on Trichosanthes primarily focus on optimization of culture protocols, with the occurrence of shoot tip necrosis (STN) seldom reported, only mentioned in the case of Trichosanthes dioica Roxb.…”