Micropropagation of Cynara scolymus L. employing cyclodextrins to promote rhizogenesis

Brutti, C.; Apóstolo, Nancy Mariel; Ferrarotti, Susana Alicia; Llorente, Berta E.; Krymkiewicz, Norberto

doi:10.1016/s0304-4238(99)00067-9

Cited by 29 publications

(24 citation statements)

References 11 publications

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“…Essentially, it can be noted that Terom shoots grown on the perlite support showed the highest rooting ability (42.5±5%) characterized by few and long roots, while, Empolese and Chiusure shoots grown both on the agar and perlite supports showed crowded thick roots ( Figure 6). Similar rooting protocols used by Cavallaro [25] and Brutti [21] reported almost 60% of rooted plantlets on early cultivars. Genotypical differences in the response to hormonal and cyclodextrins concentration used for rooting medium may have lead to the low effectiveness shown in the cultivars studied and may indicate the necessity of specific optimization for each genotype.…”

Section: Rooting Phase and Acclimatization Phasementioning

confidence: 59%

“…It has been demonstrated [34] that cyclic oligosaccharides increase the solubility or cell-membrane permeability of auxins promoting rhizogenesis [21,25]. Table 4 and Figure 5 show the rooting parameters and the rooting performance of the four cultivars tested on this type of medium, comparing agar and perlite supports.…”

Section: Rooting Phase and Acclimatization Phasementioning

confidence: 99%

“…Normally, media employed for artichoke micropropagation contains basic MS macroelements, however modified concentrations have often been used. For artichoke micropropagation, Rossi and De Paoli [16] reduced all the macronutrient content to onehalf and Brutti et al [21] reduced the KNO 3 and NH 4 NO 3 concentrations to one-half. In addition, previous studies [10,14,16,22,24] showed that low concentrations of plant growth regulators improved the quality of explants, while higher concentrations caused an increase in the shoot hyperhydricity and browning.…”

Section: Induction Phasementioning

confidence: 99%

“…This method, with the presence of paclobutrazol (PBZ) in the medium, was selected to prepare and stimulate the induction of adventitious roots [25]. Each shoot was then rooted in the basic medium proposed by Brutti et al [21]. During the pre-rooting phase, the shoots of all cultivars had typical deeply lobed, silvery, glaucousgreen leaves.…”

Section: Rooting Phase and Acclimatization Phasementioning

confidence: 99%

“…A comparison of different types of rooting media was made either by changing mineral or hormonal compositions [18,20], by adding alternative components such as cyclodextrines [21], or by employing mycorrhizae [22][23][24][25]. The effects of physical modifications in the vessel microenvironment, on rooting processes, have also been investigated [26].…”

Section: Introductionmentioning

confidence: 99%

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Plant tissue cultures from four Tuscan globe artichoke cultivars

et al. 2012

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The aim of the study was to examine the possibility of propagating in vitro four of the most common cultivars in Tuscany (central Italy): Terom, Violetto di Toscana, Chiusure and Empolese. The first three belong to the “Violetti” group, while cv Empolese belongs to the “Romaneschi” group. Explants were cultured on an induction medium (IM), which is a modified MS medium consisting of nitrate concentrations reduced by one quarter, 0.8 mg L−1 6-benzylaminopurine (BA) and 0.2 mg L−1 3-indole butyric acid (IBA). Explants were then transferred to a proliferation medium (PM) consisting of the same basal medium together with 0.03 mg L−1 BA and 0.05 mg L−1 gibberellic acid (GA3). A rooting double-phase was then established. The pre-rooting medium (PRM), consisting of a basal MS medium with half strength nitrate concentrations, 0.5 mg L−1 indole-3-acetic acid (IAA) and 1 mg L−1 paclobutrazol (PBZ) was used for two weeks. Over the next four weeks, a rooting medium (MR) was used, consisting of a basal MS medium with 2 mg L−1 β-cyclodextrin and 2 mg L−1 α-naphthaleneacetic acid sodium salt (NAA). The cv Empolese provided the highest number of proliferated explants and rooted plantlets using the method described.

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Section: Rooting Phase and Acclimatization Phasementioning

confidence: 59%

Section: Rooting Phase and Acclimatization Phasementioning

confidence: 99%

Section: Induction Phasementioning

confidence: 99%

Section: Rooting Phase and Acclimatization Phasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plant tissue cultures from four Tuscan globe artichoke cultivars

et al. 2012

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Micropropagation of Globe Artichoke (Cynara cardunculus L. var. scolymus)

Iapichino

2012

Methods in Molecular Biology

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The globe artichoke (Cynara cardunculus L. var. scolymus) is a perennial plant cultivated in the Mediterranean region and the Americas for its edible young flower heads. Although vegetative propagation by offshoots or by "ovoli" (underground dormant axillary buds) has been the primary method of propagation, the potential for the diffusion of diseases and the phenotypic variability can be very high. The propagation of this species by axillary shoot proliferation from in vitro-cultured meristems produces systemic pathogen-free plants and a higher multiplication rate as compared to that obtained by conventional agamic multiplication. Axillary shoot proliferation can be induced from excised shoot apices cultured on Murashige and Skoog agar solidified medium supplemented with various concentrations of cytokinins and auxins, depending on genotype. For the production of virus-free plants, meristems, 0.3-0.8 mm long are excised from shoot apices and surface sterilized. The transfer of artichoke microshoots to a medium lacking cytokinins or with low cytokinin concentration is critical for rooting. Adventitious roots develop within 3-5 weeks after transfer to root induction MS medium containing NAA or IAA at various concentrations. However, in vitro rooting frequency rate is dependent on the genotype and the protocol used. Acclimatization of in vitro microshoots having 3-4 roots is successfully accomplished; plantlets develop new roots in ex vitro conditions and continue to grow.

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