Globe Artichoke Tissue Culture and Its Biotechnological Application

Comino, Cinzia; Moglia, Andrea; Repetto, A.; Tavazza, Raffaela

doi:10.1007/978-3-030-20012-1_3

Cited by 4 publications

(5 citation statements)

References 76 publications

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“…While plant regeneration protocols from undifferentiated cell cultures (also from friable calluses) were successful for many different—including non-model—species (reviewed recently by [ 96 ]), cardoon or other Cynara species have proven recalcitrant to regeneration [ 97 ]. Cell culture-based biofactories allow for the overcoming of the difficulties of obtaining whole transgenic plants from cardoon and related species; moreover, their use is not affected by the strict genetically modified organism (GMO) legislation of several countries.…”

Section: Discussionmentioning

confidence: 99%

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Paolo

Locatelli

Cominelli

et al. 2021

IJMS

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Cultivated cardoon (Cynara cardunculus var. altilis L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for Agrobacterium tumefaciens-mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the Arabidopsis thaliana MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of AtMYB4 overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.

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Section: Discussionmentioning

confidence: 99%

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Paolo

Locatelli

Cominelli

et al. 2021

IJMS

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“…Accordingly, the bract tissue has been explored in different plant species for its capacity to induce in vitro mass adventitious plants. Although previous results varied, ranging from mere protoplast in banana (Matsumoto et al, 1988) and callus in gladiolus (Bajaj et al, 1983) development to complete organismal development in banana and globe artichoke (Smitha et al, 2017;Comino et al, 2019), the interest in bract tissue culture is growing. The present results are encouraging and show similarities to standard explants that are commonly applied in tissue culture.…”

Section: Discussionmentioning

confidence: 99%

“…The present results are encouraging and show similarities to standard explants that are commonly applied in tissue culture. For example, the use of bract tissue has led to plant development via both direct and indirect shoot organogenesis in different plant species (Ordas et al, 1990;Ninghui et al, 2001;Comino et al, 2019). Other studies have shown that plants can be produced in bract tissue culture via somatic embryogenesis (Divakaran and Nair, 2011;Smitha et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

In vitro daylily (Hemerocallis species) bract multiple shoot induction

Matand¹,

Shoemake²,

Li³

2021

Afr. J. Biotechnol.

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A bract is a non-inflorescence structure that exists in many plant species. In daylilies, the bract is biologically functional and is the object of the present tissue culture study. After sterilization, bract explants were treated with 6-benzylaminopurine (BA) and thidiazuron (TDZ) that were used individually or in combination in Murashige and Skoog (MS) nutrients medium, under room environmental conditions, to study its capacity to induce shoots in vitro. The results were successful. Both direct and indirect shoot organogenesis were observed. Although variably, all nineteen cultivars that were investigated induced multiple shoots. TDZ was the most potent chemical stimulus for shoot organogenesis. The results also showed no significant correlation between shoot conversion potential and genotype or treatment.

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“…Artificial light is used during in vitro culture, although the intensity and wavelength differ from those of sunlight. The daily photoperiod is identical, typically 16 h of day and 8 h of night (Comino et al 2019), and the light does not increase and diminish gradually as the sun rises or sets. These artificial conditions suggest that the expression of genes regulated by light-responsive elements is altered and prolonged exposure to abnormal lighting may cause long-term adaptation of the plant through mutations.…”

Section: Promotersmentioning

confidence: 99%

Molecular insight into somaclonal variation phenomena from transcriptome profiling of cucumber (Cucumis sativus L.) lines

Pawełkowicz

Skarzyńska

Mróz

et al. 2021

Plant Cell Tiss Organ Cult

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Somaclonal variation during in vitro culture is often an undesirable phenomenon but may also be a source of genetic variation useful for breeders. The molecular mechanisms underlying this phenomenon remain uncertain. In this study, we analyzed the fruit transcriptome of three independent cucumber lines using RNA-sequencing technology and performed an extensive in silico study to determine how somaclonal variation altered gene expression. Comparison of the transcriptome profiles with the wild-type cucumber ‘Borszczagowski B10’ revealed 418, 364, and 273 genes that were differentially regulated. We performed bioinformatic functional analysis, gene ontology classification, molecular network analysis, and explored differentially expressed genes associated with processes such as protein and nucleic acid binding, enzyme activity, signaling, transport, sugar and lipid metabolism. We determined that the differential gene expression may be caused by polymorphism in the genic region and may also be a result of interaction among molecular networks, which triggers specific pathways.

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Globe Artichoke Tissue Culture and Its Biotechnological Application

Cited by 4 publications

References 76 publications

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

In vitro daylily (Hemerocallis species) bract multiple shoot induction

Molecular insight into somaclonal variation phenomena from transcriptome profiling of cucumber (Cucumis sativus L.) lines

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