In vitro daylily (Hemerocallis species) bract multiple shoot induction

Abstract

A bract is a non-inflorescence structure that exists in many plant species. In daylilies, the bract is biologically functional and is the object of the present tissue culture study. After sterilization, bract explants were treated with 6-benzylaminopurine (BA) and thidiazuron (TDZ) that were used individually or in combination in Murashige and Skoog (MS) nutrients medium, under room environmental conditions, to study its capacity to induce shoots in vitro. The results were successful. Both direct and indirect shoot organogenesis were observed. Although variably, all nineteen cultivars that were investigated induced multiple shoots. TDZ was the most potent chemical stimulus for shoot organogenesis. The results also showed no significant correlation between shoot conversion potential and genotype or treatment.

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

“…Therefore, more studies are encouraged to determine the related threshold concentration. The importance of cytokinin is not limited to daylily micropropagation as its application has significantly promoted in vitro multiplication [36,37]. In the rooting stage, the number of roots and root length were better at the low NAA concentration (0.5 mg/l) than at the higher NAA concentrations indicating that daylily tissues may contain a high quantity of auxins.…”

“…Occasionally reported techniques that are usually unreliable or challenging to reproduce are frequently used to support this idea. As a result, attempts to enhance the crop using recent techniques like genetic modification and/or CRISPR/Cas9 editing [10][11][12][13] have been hampered. The daylily plant naturally makes a few extra divisions during each growing season.…”