Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb.

Abstract

Micropropagation through cotyledonary and leaf node and boswellic acid production in stem callus of a woody medicinal endangered tree species Boswellia serrata Roxb. is reported. The response for shoots, roots and callus formation were varied in cotyledonary and leafy nodal explants from in vitro germinated seeds, if inoculated on Murshige and Skoog's (MS) medium fortified with cytokinins and auxins alone or together. A maximum of 8.0 ± 0.1 shoots/cotyledonary node explant and 6.9 ± 0.1 shoots/leafy node explants were produced in 91 and 88 % cultures respectively on medium with 2.5 μM 6-benzyladenine (BA) and 200 mg l(-1) polyvinylpyrrolidone (PVP). Shoots treated with 2.5 μM IBA showed the highest average root number (4.5) and the highest percentage of rooting (89 %). Well rooted plantlets were acclimatized and 76.5 % of the plantlets showed survival upon transfer to field conditions. Randomly amplified polymorphic DNA (RAPD) analysis of the micropropagated plants compared with mother plant revealed true-to-type nature. The four major boswellic acid components in calluses raised from root, stem, cotyledon and leaf explants were analyzed using HPLC. The total content of four boswellic acid components was higher in stem callus obtained on MS with 15.0 μM IAA, 5.0 μM BA and 200 mg l(-1) PVP. The protocol reported can be used for conservation and exploitation of in vitro production of medicinally important non-steroidal anti-inflammatory metabolites of B. serrata.

Plant Regeneration through Callus Cultures from Leaf Explant of Sumatra Benzoin (Styrax benzoin)

“…ese results indicate that the ratio of NAA and BAP concentrations is crucial in inducing Sumatra Benzoin plantlets. BAP functions as an NAA antagonist in root formation, as previously reported [31][32][33][34].…”

In vitro propagation of Campomanesia rufa: An endangered fruit species

“…In the callogenesis, the results showed a linear behavior, with an increasing growth until reaching the maximum point at the highest concentration of 2,4-D tested, i.e., the highest callus formation occurred at the concentration of 10μM 2,4-D with an average rate of 58.7% calluses ( Figure 5B). The application of PVP in culture medium as antioxidant in the in vitro propagation of woody species has been previously reported in Boswellia serrata (Nikam et al, 2013) and Jatropha curcas L (Pequeño-Granado et al, 2015).…”

Chronic ethanol consumption-induced hepatotoxicity and protective effect of Boswellia serrata

“…Previous report demonstrated that alkaloids, [22] tannins, [13] flavonoids, [23] amino acids, [24] and glycosides [25] may protect hepatic damage.…”