Micronucleus test in freshwater fish species: an evaluation of its sensitivity for application in field surveys

Rodríguez-Cea, Andrés

doi:10.1016/s0147-6513(03)00073-3

Cited by 98 publications

(61 citation statements)

References 26 publications

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“…Similar results were described in studies carried out with other fish species establishing in situ frequencies (Rodriguez-Cea et al, 2003;Grisolia et al, 2009). The highest baseline MN and NA frequencies were found in the species S. brevipinna.…”

Section: Discussionsupporting

confidence: 89%

“…In this way, several studies of biomonitoring in situ in rivers and lakes were accomplished using the micronucleus test with diverse fish species bringing about interspecific variation in baseline frequencies of genetic damage (Grisolia et al, 2009;Rodriguez-Cea et al, 2003). However, researches employing biomarkers in tropical and neotropical freshwater fish from South America are scarce (Rabitto et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Baseline micronuclei and nuclear abnormalities frequencies in native fishes from the Paraná River (Argentina)

et al. 2014

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This work aims to establish baseline frequencies of micronuclei (MN) and nuclear abnormalities (NA) in native fish species collected in situ from the Paraná River. For this purpose, the micronucleus test was applied in peripheral blood erythrocytes from specimens obtained from samplings collected at two localities (Posadas and Candelaria, Misiones, Argentina) during the period 2007-2010. The results were statistically analyzed using the Kruskal Wallis test. Data from nine fish species were obtained, among which Steindachnerina brevipinna (Characiformes) revealed the highest baseline frequency of MN and NA, showing statistically significant differences with regard to the other analyzed species. These results are the first report of baseline MN and NA frequencies for native fish species studied and could be useful for future comparisons with data of fishes belonging to other environments.Keywords: biomonitoring, micronuclei, nuclear abnormalities, fishes, Paraná River. Frequências basais de micronúcleos e anormalidades nucleares

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Baseline micronuclei and nuclear abnormalities frequencies in native fishes from the Paraná River (Argentina)

et al. 2014

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“…In wild fish, the quantification of ENMAs was proved useful to understand the type/intensity of chemical impacts in health (Rodriguez-Cea et al 2003). Those abnormalities are also regarded as indicators of cytotoxic/genotoxic damage in fish and, therefore, they are viewed as useful complements to MN scoring in genotoxicity surveys (Çavas and ErgeneGözükara 2005b).…”

Section: Introductionmentioning

confidence: 99%

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Carrola

Santos

Rocha

et al. 2014

Environ Sci Pollut Res

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Fish are bioindicators of water pollution, and an increased rate of their erythrocyte nuclear morphological abnormalities (ENMAs)-and particularly of erythrocyte micronuclei (EMN)-is used as a genotoxicity biomarker. Despite the potential value of ENMAs and MN, there is scarce information about fish captured in Iberian estuaries. This is the case of the Portuguese estuaries of the Mondego, Douro and Ave, suffering from different levels of environmental stress and where chemical surveys have been disclosing significant amounts of certain pollutants. So, the aim of this study was to evaluate genotoxicants impacts and infer about the exposure at those ecosystems, using the grey mullet (Mugil cephalus) as bioindicator and considering the type and frequency of nuclear abnormalities of erythrocytes as proxies of genotoxicity. Sampling of mullets was done throughout the year in the important Mondego, Douro and Ave River estuaries (centre and north-western Portugal). The fish (total n=242) were caught in campaigns made in spring-summer and autumnwinter, using nets or fishing rods. The sampled mullets were comparable between locations in terms of the basic biometric parameters. Blood smears were stained with Diff-Quik to assess the frequencies of six types of ENMAs and MN (given per 1,000 erythrocytes). Some basic water physicochemical parameters were recorded to search for fluctuations matching the ENMAs. Overall, the most frequent nucleus abnormality was the polymorphic type, sequentially followed by the blebbed/ lobed/notched, segmented, kidney shaped, vacuolated, MN and binucleated. The total average frequency of the ENMAs ranged from 73‰ in the Mondego to 108‰ in the Ave. The polymorphic type was typically ≥50 % of the total ENMAs, averaging about 51‰, when considering all three estuaries. The most serious lesion-the MN-in fish from Mondego and Douro had a similar frequency (≈0.38‰), which was significantly lower than that in the Ave (0.75‰). No significant seasonal differences existed as to the MN rates and seasonal differences existed almost only in the Douro, with the higher values in AW. In general, the pattern of ENMAs frequencies was unrelated with the water physicochemical parameters. Considering the data for both the total ENMAs and for each specific abnormality, and bearing in mind that values of MN in fish erythrocytes >0.3‰ usually reflect pollution by genotoxicants, it is suggested that mullets were likely being chronically exposed to such compounds, even in the allegedly less polluted ecosystem (Mondego). Moreover, data supported the following pollution

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“…The assay is well elaborated and allows to evaluate the genotoxicity of compounds at low concentrations and to assess doseresponse relationships of both DNA reactive and non-DNA reactive genotoxins (e.g. aneugens) (Al-Sabti et al, 1994;Palhares and Grisolia, 2002;Pacheco and Santos, 2002;Santos, 2002, 2003;Rodriguez-Cea et al, 2003;Teles et al, 2003) Micronuclei arise from chromosome fragments or whole chromosomes that lag at cell division due to lack of centromere, damage in centromere region, or defect in cytokinesis (Fenech, 2000). MN are small, secondary structures of chromatin, are surrounded by membranes, located in the cytoplasm and have no detectable link to the cell nucleus (Boller and Schmid, 1970;Heddle, 1973;Heddle et al, 1991;MacGregor, 1991).…”

Section: Introductionmentioning

confidence: 99%

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Rybakovas

Baršienė

Lang

2009

Marine Environmental Research

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Please cite this article as: Rybakovas, A., Baršienė, J., Lang, T., Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and North Seas, Marine Environmental Research (2009Research ( ), doi: 10.1016Research ( /j.marenvres. 2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractMicronuclei (MN), nuclear buds (NB) and fragmented-apoptotic cells (FA) were analyzed in mature peripheral blood and immature cephalic kidney erythrocytes of flounder (Platichthys flesus), dab (Limanda limanda) and cod (Gadus morhua) from 12 offshore sites in the Baltic Sea (479 specimens) and 11 sites in the North Sea (291 specimens), which were collected during three research vessel cruises in December 2002 and in September 2004. The highest levels of environmental genotoxicity (frequencies of MN up to 0.5 ‰, NB -up to 0.75 ‰) and cytotoxicity (FA -up to 0.53 ‰) were observed in flatfishes from areas close to oil and gas platforms in the North Sea and in zones related to the extensive shipping and potentially influenced by contamination from large European Rivers (Elbe, Vistula, Oder). In dab from the offshore zones of the North Sea, the levels of nuclear abnormalities were higher as compared to those in dab from the Baltic Sea. Responses in immature kidney erythrocytes were higher than in mature erythrocytes from peripheral blood. MN frequency lower than 0.05 ‰ (the Baltic Sea) and lower than 0.1 ‰ (the North Sea) could be suspected as a reference level in the peripheral blood erythrocytes of flatfish.Keywords: Genotoxicity; Cytotoxicity; Micronuclei; Nuclear abnormalities; Erythrocytes; Baltic Sea; North Sea. ACCEPTED MANUSCRIPT 2 IntroductionSurrounded by European countries, the Baltic and the North Seas are constantly exposed to anthropogenic pressure. With pollution from land-based discharges, coastal industries, municipal outflows, agricultural activities, oil and gas industry (drilling and transportation) and transport activities, thousands of chemicals are entering the marine environment. A large proportion of these compounds are potentially toxic, genotoxic and carcinogenic to aquatic organisms (Jha, 2004(Jha, , 2008. The interaction of genotoxic contaminants with DNA causes various genetic disturbances, which often are irreversible and can be transmitted to the next generations (Depledge and Fossi, 1994;Dixon et al., 1999;Jha, 2004). The analysis of environmental genotoxicity provides early warning signs of adverse long-term effects of contamination. A number of biomarkers have been applied for assessment of environmental genotoxicity and the micronuclei (MN) test,...

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Micronucleus test in freshwater fish species: an evaluation of its sensitivity for application in field surveys

Cited by 98 publications

References 26 publications

Baseline micronuclei and nuclear abnormalities frequencies in native fishes from the Paraná River (Argentina)

Baseline micronuclei and nuclear abnormalities frequencies in native fishes from the Paraná River (Argentina)

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

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