Micronucleus assay of buccal mucosa cells in smokers with the history of smoking less and more than 10 years

Background:Ionizing radiation is a potent mutagenic agent capable of inducing both mutation and chromosomal aberrations. Non-lethal doses of ionizing radiation may induce genomic instability favoring carcinogenesis. In spite of their mutagenic potential, this kind of radiation is an important tool for diagnosis of the disease and is used in medical and dental practice. It has been believed that the number of micronucleus and increased frequency of other nuclear alterations, including karyorrhexis, condensed chromatin and pyknosis, are related to the increasing effects of carcinogens. Many approaches and techniques have been developed for the monitoring of human populations exposed to various mutagens, but the analysis of micronuclei (MN) has become a standard approach for the assessment of chromosomal damage in human populations.Aim:To assess the effects of radiation exposure from panoramic radiography on the buccal epithelial cells (BECs) of pediatric patients.Materials and Methods:The study included 20 pediatric patients who had to undergo panoramic radiography for further dental treatment. Exfoliated BECs were obtained and examined immediately before and 10 days after radiation exposure. The cells were stained using rapid Papanicolaou (PAP) kit. Evaluation for MN and nuclear alterations was carried out by an oral pathologist and data were statistically analyzed using the “t” test.Results:The mean number of MN in the BECs before exposure of pediatric patients to panoramic radiography was 4.25 and after exposure was 4.40. This difference was not found to be statistically significant (P < 0.0001). However, the mean nuclear alterations of 8.70 and 15.75 before and after exposure were statistically significant (P < 0.0001).Conclusion:Panoramic radiographs can induce cytotoxicity but not genotoxic effects in buccal mucosal cells. Hence, dental radiographs should be prescribed only when deemed indispensable.

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“…It has been believed that the number of MN is related to increasing the effects of carcinogens. [ 21 ]…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and cytotoxic effects of X-ray on buccal epithelial cells following panoramic radiography: A pediatric study

Agarwal

Vinuth

Haranal

et al. 2015

J Cytol

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“…The exclusion criteria in nonsmokers and smokers were existing history of smoking or quitting the smoking in past three years and smoking for less than three years ( 15 - 16 ).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Smoking on Mast Cells Density and Angiogenesis in Chronic Periodontitis

Naderi¹,

Semyari²,

Hemmati³

2017

Iran J Pathol

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Background and objective: Gingival bleeding reduction in smokers has been associated with decreased blood vessel density. The mechanism of suppressive effect of cigarette smoking on blood vessel density is not precisely defined. The aim of this study was to evaluate the impact of smoking on angiogenesis by assessing mast cells density and VEGF expression in chronic periodontitis. Materials& Methods: 52 paraffin embedded block of gingiva tissues with periodontitis obtained from 30 nonsmokers and 22 smokers undergoing flap surgery were examined immunohistochemically for VEGF expression. Mast cell counts was completed on toluidine blue stained slides. Exposure to cigarette smoking was calculated by the number of packs × year. Patients were classified into 4 groups based on the number of smoked cigarettes. The correlation between VEGF expression and mast cell counts was evaluated and compared in nonsmokers and smokers. Results: The mean number of mast cells (p=0.004) and average value of VEGF expression (p = 0.000) in nonsmokers was significantly higher than smokers. No correlation was noted between VEGF expression / mast cell counts and number of smoked cigarettes in four groups of smokers (p=0.29,0.12 , 0.20 and 0.11, respectively). Conclusion: Mast cells and VEGF expression may account for suppressive effect of cigarette smoking on blood vessels in periodontitis.

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“…Exposure to cigarette and waterpipe smoking was considered by the number of pack × years ( P × Y). [ 1 ]…”

Section: Methodsmentioning

confidence: 99%

“…It has been established that the genotoxic abnormalities of buccal mucosa in cigarette and waterpipe smokers is more than nonsmokers. [ 1 2 ] The genotoxic effects have been reported to be associated with nucleus abnormalities. [ 1 2 3 4 5 ] Spite recognized genotoxic effect[ 6 7 ] evidence on cytotoxic effect of cigarette and waterpipe smoking is very limited and controversial.…”

Section: Introductionmentioning

confidence: 99%

Comparison of cytotoxic effect of cigarette and waterpipe smoking on human buccal mucosa

Naderi

Pasha

2017

Int J Prev Med

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Background:The evidences on cytotoxic effect of cigarette and waterpipe smoking are very rare and controversial. The aim was to compare the cytotoxic effect of cigarette and waterpipe smoking on human buccal mucosa cells.Methods:The study was case–control. Feulgen-stained samples of exfoliated buccal mucosa cells were evaluated. The cytology slides of 25 cigarette smoker, 25 waterpipe smoker, and 25 individuals in the never smoked were examined. The number of pyknosis, karyorrhexis, and karyolysis in 1000 cells/subject were counted. Exposing to cigarette and waterpipe smoke was considered by the number of pack × years.Results:There were significant differences among the groups in terms of karyolysis and pyknosis while there was no significant difference among the cigarette smokers group and waterpipe smokers group in terms of karyorrhexis (P ≤ 0. 01). The cytotoxicity effect of cigarette smoking was not significantly correlated to time exposure (r = 0.099, P = 0.637). The cytotoxicity effect of waterpipe smoking was significantly correlated to time exposure (r = −370, P = 0.044).Conclusions:The cytotoxic effect of cigarette and waterpipe smoking on buccal mucosa cells was significantly higher than nonsmokers. The effect of cigarette smoking on cellular death was higher than waterpipe. The cytotoxic effect of waterpipe smoking was dose dependent.

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Micronucleus assay of buccal mucosa cells in smokers with the history of smoking less and more than 10 years

Cited by 39 publications

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Genotoxic and cytotoxic effects of X-ray on buccal epithelial cells following panoramic radiography: A pediatric study

Genotoxic and cytotoxic effects of X-ray on buccal epithelial cells following panoramic radiography: A pediatric study

The Effect of Smoking on Mast Cells Density and Angiogenesis in Chronic Periodontitis

Comparison of cytotoxic effect of cigarette and waterpipe smoking on human buccal mucosa

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