Comparison of cytotoxic effect of cigarette and waterpipe smoking on human buccal mucosa

Naderi, Noushin Jalayer; Pasha, Mona Pour

doi:10.4103/ijpvm.ijpvm_62_17

Cited by 13 publications

(2 citation statements)

References 21 publications

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“…[ 23 ] The genomic changes that occur at the onset of exposure to these agents further progress to cytotoxic changes because these mutagenic agents stimulate cellular death to eliminate the genotoxic damage cells. [ 24 ]…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity in oral mucosal epithelial cells of petrol station attendants: A micronucleus study

Rehani¹,

Raj²,

Jeergal³

et al. 2021

J Cytol

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Introduction: Occupational exposure to petrol derivatives possesses an increased risk of various cancers including that of the oral mucosa. Scientific studies have shown the correlation of micronuclei assay (MN) with the cytogenotoxic changes in petrol station attendants. However, very few have reported the use of MN assay as a promising tool for assessing the impact of smoking in these workers. Aim: To explore the cytogenotoxic damage in exfoliated buccal cells obtained from petrol station attendants and control subjects using the MN assay along with additional effects due to smoking. Materials and Methods: The study comprised 60 males who were divided into Group I–IV with each having 15 subjects. These subjects were categorized as exposed smokers, exposed nonsmokers, unexposed smoker group, and unexposed nonsmokers. The MN and additional nuclear abnormalities (karyorrhexis [KH], binucleation [BN], pyknosis [P], and karyolysis [KL]) were calculated in PAP-stained slides. Results: Statistically higher mean frequencies of overall nuclear anomalies were observed in petrol pump workers in comparison with the control group. Petrol pump smokers carry the highest nuclear anomalies followed by non-exposed smokers than exposed non-smokers and the count was the least among unexposed non-smoker workers. Discussion and Conclusion: The present study indicated that the petrol pump workers are under higher cytogenotoxic damage. Also, smoking added to the frequency of damage. Thus, MN and other nuclear anomalies are in-vitro reliable biomarker assays available and should be routinely employed as a screening tool in their periodic medical evaluation.

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Section: Discussionmentioning

confidence: 99%

Genotoxicity in oral mucosal epithelial cells of petrol station attendants: A micronucleus study

Rehani¹,

Raj²,

Jeergal³

et al. 2021

J Cytol

View full text Add to dashboard Cite

show abstract

“… 4 The cellular and molecular mechanisms underlying these changes have not been fully elucidated and involve various degrees of inflammation-related alterations in growth rates, as well as enhancement of the nucleus/cytoplasm ratio with associated pyknosis, karyorrhexis, and karyolysis. 5 , 6 The cytotoxic effects lead to epigenomic and transcriptomic changes, followed by apoptosis. 7 …”

Section: Introductionmentioning

confidence: 99%

Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure

et al. 2020

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Objective This prospective clinical study comparatively investigated the effects of tobacco smoking on global methylation and hydroxymethylation in oral epithelial cells. Methods Buccal cells from the inside of the cheeks were collected from 47 individuals, including smokers, former smokers, and never smokers. DNA was extracted using dedicated kits. Methylated and hydroxymethylated DNA fractions were measured using assays similar to enzyme-linked immunosorbent assays. The levels of methylation and hydroxymethylation were compared among groups using unpaired two-tailed t-tests or the Mann–Whitney U test; P < 0.05 was considered statistically significant. Results There was no statistically significant difference in the average number of cigarettes between smoker and former smoker groups. Although methylation levels were lower for smokers (3.1%) and former smokers (2.16%), compared with never smokers (4.16%), these differences were not statistically significant. There was a two-fold increase in hydroxymethylation level in never smokers, compared with smokers. Conclusions Our findings suggest that smoking leads to global reductions in both methylation and hydroxymethylation levels in oral epithelial cells in a manner influenced by the intensity and length of exposure to tobacco smoke.

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The Effect of Waterpipe Smoking on DNA Integrity Among Youth in Jordan

et al. 2019

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Comparison of cytotoxic effect of cigarette and waterpipe smoking on human buccal mucosa

Cited by 13 publications

References 21 publications

Genotoxicity in oral mucosal epithelial cells of petrol station attendants: A micronucleus study

Genotoxicity in oral mucosal epithelial cells of petrol station attendants: A micronucleus study

Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure

The Effect of Waterpipe Smoking on DNA Integrity Among Youth in Jordan

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