Genotoxic and cytotoxic effects of X-ray on buccal epithelial cells following panoramic radiography: A pediatric study

Agarwal, Poonam; Vinuth, Dhundanalli Puttalingaiah; Haranal, Shashidevi; Thippanna, Chandrashekar K; Naresh, N; Moger, Ganapathi

doi:10.4103/0970-9371.160559

Cited by 21 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These studies, however, all reported increases in other nuclear alterations (e.g. pyknosis, karyorrhexis and karyolysis) that are associated with increased cytotoxicity [63][64][65][66] . Recently, Preethi et al (2016) reported significant increases in the number of micronucleated cells in BMCs after dental radiography in paediatric patients 62 .…”

Section: Discussionmentioning

confidence: 95%

“…Furthermore, our group recently showed that low doses associated with CBCT examinations are capable of inducing DNA DSBs in vitro in dental stem cells 61 . BMCs have also been used successfully as a biomarker for genotoxic effects, including using the γH2AX assay to detect radiation-induced DNA DSBs 48,62,63 . These studies report increase of genotoxic effects in BMCs after low dose IR exposure.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan

Belmans

Gilles

Vermeesen

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Assessing the possible biological effects of exposure to low doses of ionizing radiation (IR) is one of the prime challenges in radiation protection, especially in medical imaging. Today, radiobiological data on cone beam CT (CBCT) related biological effects are scarce. In children and adults, the induction of DNA double strand breaks (DSBs) in buccal mucosa cells and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) and antioxidant capacity in saliva samples after CBCT examination were examined. No DNA DSBs induction was observed in children nor adults. In children only, an increase in 8-oxo-dG levels was observed 30 minutes after CBCT. At the same time an increase in antioxidant capacity was observed in children, whereas a decrease was observed in adults. Our data indicate that children and adults react differently to IR doses associated with CBCT. Fully understanding these differences could lead to an optimal use of CBCT in different age categories as well as improved radiation protection guidelines. Uncertainties concerning low dose ionizing radiation exposure and medical imaging. Currently, a debate exists within the radiation protection community about which model best reflects the relation between the ionizing radiation (IR) dose and the additional health risk. Several models have been described thus far. These include: the linear non-threshold (LNT) model, the linear threshold model, the hormetic model and the hypersensitivity model 1. Currently, the linear non-threshold (LNT) model is used to estimate risks in radiation protection guidelines. Although the LNT model is supported by epidemiological evidence in the high dose range (>100 milliGray (mGy)), increasing evidence disproves it in the low dose range 2-5. One of the main critiques is the fact that the LNT model does not take into account biological defence mechanisms (e.g. DNA repair mechanisms) 6,7. In addition, a lot of uncertainties still exist about low doses (<100 mGy), mostly because of a lack of statistical power of the epidemiological data. Knowing which of these models supports the relation between exposure to low doses of IR and the involved risk best is of importance in medical imaging applications of IR. Such applications include computed tomography (CT) and, more recently, cone beam computed tomography (CBCT), which typically uses doses far below 100 mGy, (typically between 0.01-0.10 mGy) 8-11. Multiple controversial studies indicate that exposure of children to diagnostic radiology may lead to radiation-induced malignancies later in life. Retrospective studies observed that the use of CT scans in children could triple the risk of leukaemia and brain cancers 12-14. A 24% increase in cancer incidence was seen in an

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan

Belmans

Gilles

Vermeesen

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Although the amount of released monomers is detectable and toxic in vitro, it has been concluded that it is still too small to cause long-term in vivo damage ( 23 ). The analysis of changes in the nucleus within the same period confirmed our hypothesis that there is no difference in genotoxicity between different fissure sealants.…”

Section: Discussionmentioning

confidence: 99%

“…Despite being one of the most important agents for remineralization process, fluoride has potentially genotoxic effect ( 24 , 25 ). In addition, the other variables that potentially affect the occurrence of nuclear abnormalities such as gender, age, X-rays exposures medicines intake, the consumption of meat, sweets, soda drinks and tea were analyzed ( 23 , 26 , 27 ). Previous studies have confirmed that age is a variable that affects the genetic content ( 26 , 28 ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Cytotoxic and Genotoxic Effect of Fissure Sealants in Buccal Epithelial Cells

Gavić¹,

Goršeta²,

Buterin³

et al. 2021

Acta Stomatol Croat

View full text Add to dashboard Cite

Aim The main purpose of this study was to assess the genotoxic and cytotoxic effect of fissure sealants on buccal epithelial cells. Material and methods The study was conducted on 45 patients (27 girls and 18 boys), seven to 16 years of age (age mean 12.09 ± 2.20). Buccal swabs were collected before (T0), seven (T1), 30 (T2) and 90 days (T3) consequently after fissure sealant placement (Helioseal F®, Equia Fil®, Constic®). Patients or legal guardians filled in the questionnaire regarding the demographic data (age, gender), dietary habits, health status, medication usage, and recent X-ray exposure. DNA damage was analyzed using the micronucleus test. Results Statistically significant difference in the number of buccal cells with condensed chromatin was found between T0 (time before fissure sealant placement) and T3 (90 days after fissure sealant placement) period for Helioseal F® (P = 0.025). For the other two analyzed materials, no difference was observed during the tested period. There was no difference between materials in the same sampling time. Conclusion Apart from an increase in cells with condensed chromatin 90 days after the placement of Helioseal F®, no other nuclear abnormalities were observed for tested fissure sealants. Although these sealants have now largely been used, it is of high importance that their biocompatibility is checked continuously, especially in in vivo clinical studies.

show abstract

“…The micronucleus score obtained in the current study; the pre-exposure values were having a mean ± standard deviation = 4.58 ± 1.8, which was consistent with controls who were not exposed to any genotoxins in Indian population. [ 15 16 ]…”

Section: Discussionmentioning

confidence: 99%

The genotoxic and cytotoxic effects of CT scan on buccal epithelial cells

Palla¹,

Rangdhol²,

Uma

et al. 2020

J Cytol

View full text Add to dashboard Cite

Background: Diagnostic radiation is reported to cause significant damage in buccal cells, while the same effects after natural cell turn over cycle were not checked for in previous studies. The buccal cells were studied in patients exposed to computed tomography (CT) scans for evaluating the cells with micronuclei and cytotoxic changes, namely, pyknotic cells, karyorrhectic cells and karyolytic cells. The pre-exposure counts were compared with postexposure counts on 10 and 20 days corresponding to first and second cell turnover cycles. Aim: The aim of this study is to estimate the counts of micronucleus and cytotoxic changes in buccal cells post-exposure to CT scans and report on variance of the same with first and second buccal cell turnover cycles. Materials and Methods: This is an observational study, wherein the buccal smears of patients undergoing CT scans were made before and after CT scan exposures as needed. Papanicolaou (PAP) staining and analysis were performed as per standard criteria for micronuclear and cytotoxic changes, respectively. Statistical test used was paired t -tests. Results: The micronuclear counts revealed 0.4% positive cells before exposure and 1.4% positive cells post 10 days and 20 days of exposure were significant ( P < 0.005). The cytotoxic changes showed around 2.5% positive cells before and 5.7% positive cells 10 days after CT exposure ( P < 0.005). The cytotoxic cell values from baseline to 20 th day were not significant ( P < 0.25). Conclusion: CT scans have caused genotoxic effects notable after two cell turnover cycles but the cytotoxic changes have significantly decreased naturally after 2 nd cell turnover as per our study.

show abstract

Genotoxic and cytotoxic effects of X-ray on buccal epithelial cells following panoramic radiography: A pediatric study

Cited by 21 publications

References 23 publications

Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan

Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan

Assessment of Cytotoxic and Genotoxic Effect of Fissure Sealants in Buccal Epithelial Cells

The genotoxic and cytotoxic effects of CT scan on buccal epithelial cells

Contact Info

Product

Resources

About